Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium

Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium

Optimization of decolorization of methylene blue (MB) dye by lignin peroxidase (LiP) enzyme produced by white-rot fungus Phanerochaete chrysosporium using sewage treatment plant (STP) sludge as a major substratewas carried out in the laboratory.Optimization by the one-factor-at-a-time (OFAT) and sta...

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Main Authors: Alam, Md. Zahangir, Mansor, Mariatul F., Khan Chowdhury, Ahmed Jalal
Format: Article
Language:English
Published: Elsevier Science 2009
Subjects:
QP Physiology
TP Chemical technology
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/5064/
http://irep.iium.edu.my/5064/
http://irep.iium.edu.my/5064/
http://irep.iium.edu.my/5064/1/Paper-decolorization-JHM.pdf
id iium-5064
recordtype eprints
spelling iium-50642017-12-07T04:37:26Z http://irep.iium.edu.my/5064/ Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal QP Physiology TP Chemical technology TP248.13 Biotechnology Optimization of decolorization of methylene blue (MB) dye by lignin peroxidase (LiP) enzyme produced by white-rot fungus Phanerochaete chrysosporium using sewage treatment plant (STP) sludge as a major substratewas carried out in the laboratory.Optimization by the one-factor-at-a-time (OFAT) and statistical approachwas carried out to determine the process conditions on optimum decolorization ofMBdye using LiP enzyme in static mode. The OFAT method indicated that the optimum conditions for decolorization of MBdye (removal: 14–40%)was at temperature 55 ◦C, pH 5.0 with hydrogen peroxide (H2O2) concentration 4.0mM, MB dye concentration 20 mg/L and LiP activity 0.487 U/ml. The addition of veratryl alcohol to the reaction mixtures did not contribute any further increases in decolorization. The initial concentration of MB and the activity of LiP enzyme were further optimized using response surface methodology (RSM).The contour and surface plots suggested that the optimum initial concentration of MB and LiP activity predicted were 15 mg/L and 0.687 U/ml, respectively for the removal of 65%. The validation of the model showed that the decolorization process gave the higher removal of 90% in agitation mode compared to the static mode with 65% for 60 min of incubation time by LiP enzyme. Elsevier Science 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5064/1/Paper-decolorization-JHM.pdf Alam, Md. Zahangir and Mansor, Mariatul F. and Khan Chowdhury, Ahmed Jalal (2009) Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium. Journal of Hazardous Materials, 162 (2-3). pp. 708-715. ISSN 03043894 http://dx.doi.org/10.1016/j.jhazmat.2008.05.085 doi:10.1016/j.jhazmat.2008.05.085
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QP Physiology
TP Chemical technology
TP248.13 Biotechnology
spellingShingle QP Physiology
TP Chemical technology
TP248.13 Biotechnology
Alam, Md. Zahangir
Mansor, Mariatul F.
Khan Chowdhury, Ahmed Jalal
Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium
description Optimization of decolorization of methylene blue (MB) dye by lignin peroxidase (LiP) enzyme produced by white-rot fungus Phanerochaete chrysosporium using sewage treatment plant (STP) sludge as a major substratewas carried out in the laboratory.Optimization by the one-factor-at-a-time (OFAT) and statistical approachwas carried out to determine the process conditions on optimum decolorization ofMBdye using LiP enzyme in static mode. The OFAT method indicated that the optimum conditions for decolorization of MBdye (removal: 14–40%)was at temperature 55 ◦C, pH 5.0 with hydrogen peroxide (H2O2) concentration 4.0mM, MB dye concentration 20 mg/L and LiP activity 0.487 U/ml. The addition of veratryl alcohol to the reaction mixtures did not contribute any further increases in decolorization. The initial concentration of MB and the activity of LiP enzyme were further optimized using response surface methodology (RSM).The contour and surface plots suggested that the optimum initial concentration of MB and LiP activity predicted were 15 mg/L and 0.687 U/ml, respectively for the removal of 65%. The validation of the model showed that the decolorization process gave the higher removal of 90% in agitation mode compared to the static mode with 65% for 60 min of incubation time by LiP enzyme.
format Article
author Alam, Md. Zahangir
Mansor, Mariatul F.
Khan Chowdhury, Ahmed Jalal
author_facet Alam, Md. Zahangir
Mansor, Mariatul F.
Khan Chowdhury, Ahmed Jalal
author_sort Alam, Md. Zahangir
title Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium
title_short Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium
title_full Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium
title_fullStr Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium
title_full_unstemmed Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium
title_sort optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with phanerocheate chrysosporium
publisher Elsevier Science
publishDate 2009
url http://irep.iium.edu.my/5064/
http://irep.iium.edu.my/5064/
http://irep.iium.edu.my/5064/
http://irep.iium.edu.my/5064/1/Paper-decolorization-JHM.pdf
first_indexed 2023-09-18T20:13:33Z
last_indexed 2023-09-18T20:13:33Z
_version_ 1777407637176975360

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