Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR

Background: Synovial sarcoma (SS) is a rare cancer and accounts for 5-10% of adult soft tissue sarcomas. Making an accurate diagnosis is difficult due to the overlapping histological features of SS with other types of sarcomas and the non-specific immunohistochemistry profile findings. Molecular...

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Main Authors:	A.Talib, Norlelawati, Goon, Mohd Danial, Harun, Nora, Othman, Nadia, Kamaruzaman, Zatur Rawihah, Abdullah, Nor Zamzila, Muhammad, Siti Aesah @ Naznin
Format:	Article
Language:	English English
Published:	College of Pathologists, Academy of Medicine Malaysia 2016
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/50533/ http://irep.iium.edu.my/50533/ http://irep.iium.edu.my/50533/1/detection-of-SYT-SSX.pdf http://irep.iium.edu.my/50533/4/50533_Detection_of_SYT-SSX_mutant_transcripts_in_formalin-fixed_SCOPUS.pdf

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recordtype	eprints
spelling	iium-505332018-02-25T09:20:02Z http://irep.iium.edu.my/50533/ Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR A.Talib, Norlelawati Goon, Mohd Danial Harun, Nora Othman, Nadia Kamaruzaman, Zatur Rawihah Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin RB Pathology Background: Synovial sarcoma (SS) is a rare cancer and accounts for 5-10% of adult soft tissue sarcomas. Making an accurate diagnosis is difficult due to the overlapping histological features of SS with other types of sarcomas and the non-specific immunohistochemistry profile findings. Molecular testing is thus considered necessary to confirm the diagnosis since more than 90% of SS cases carry the transcript of t(X;18)(p11.2;q11.2). The purpose of this study is to diagnose SS at molecular level by testing for t(X;18) fusion-transcript expression through One-step reverse transcriptase real-time Polymerase Chain Reaction (PCR). Method: Formalin-fixed paraffin-embedded tissue blocks of 23 cases of soft tissue sarcomas, which included 5 and 8 cases reported as SS as the primary diagnosis and differential diagnosis respectively, were retrieved from the Department of Pathology, Tengku Ampuan Afzan Hospital, Kuantan, Pahang. RNA was purified from the tissue block sections and then subjected to One-step reverse transcriptase real-time PCR using sequence specific hydrolysis probes for simultaneous detection of either SYT-SSX1 or SYT-SSX2 fusion transcript. Results: Of the 23 cases, 4 cases were found to be positive for SYT-SSX fusion transcript in which 2 were diagnosed as SS whereas in the 2 other cases, SS was the differential diagnosis. Three cases were excluded due to failure of both amplification assays SYT-SSX and control β-2-microglobulin. The remaining 16 cases were negative for the fusion transcript. Conclusion: This study has shown that the application of One-Step reverse transcriptase real time PCR for the detection SYT-SSX transcript is feasible as an aid in confirming the diagnosis of synovial sarcoma. College of Pathologists, Academy of Medicine Malaysia 2016-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/50533/1/detection-of-SYT-SSX.pdf application/pdf en http://irep.iium.edu.my/50533/4/50533_Detection_of_SYT-SSX_mutant_transcripts_in_formalin-fixed_SCOPUS.pdf A.Talib, Norlelawati and Goon, Mohd Danial and Harun, Nora and Othman, Nadia and Kamaruzaman, Zatur Rawihah and Abdullah, Nor Zamzila and Muhammad, Siti Aesah @ Naznin (2016) Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR. The Malaysian Journal of Pathology, 38 (1). pp. 11-18. ISSN 0126-8635 http://www.mjpath.org.my/2016/v38n1/detection-of-SYT-SSX.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RB Pathology
spellingShingle	RB Pathology A.Talib, Norlelawati Goon, Mohd Danial Harun, Nora Othman, Nadia Kamaruzaman, Zatur Rawihah Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR
description	Background: Synovial sarcoma (SS) is a rare cancer and accounts for 5-10% of adult soft tissue sarcomas. Making an accurate diagnosis is difficult due to the overlapping histological features of SS with other types of sarcomas and the non-specific immunohistochemistry profile findings. Molecular testing is thus considered necessary to confirm the diagnosis since more than 90% of SS cases carry the transcript of t(X;18)(p11.2;q11.2). The purpose of this study is to diagnose SS at molecular level by testing for t(X;18) fusion-transcript expression through One-step reverse transcriptase real-time Polymerase Chain Reaction (PCR). Method: Formalin-fixed paraffin-embedded tissue blocks of 23 cases of soft tissue sarcomas, which included 5 and 8 cases reported as SS as the primary diagnosis and differential diagnosis respectively, were retrieved from the Department of Pathology, Tengku Ampuan Afzan Hospital, Kuantan, Pahang. RNA was purified from the tissue block sections and then subjected to One-step reverse transcriptase real-time PCR using sequence specific hydrolysis probes for simultaneous detection of either SYT-SSX1 or SYT-SSX2 fusion transcript. Results: Of the 23 cases, 4 cases were found to be positive for SYT-SSX fusion transcript in which 2 were diagnosed as SS whereas in the 2 other cases, SS was the differential diagnosis. Three cases were excluded due to failure of both amplification assays SYT-SSX and control β-2-microglobulin. The remaining 16 cases were negative for the fusion transcript. Conclusion: This study has shown that the application of One-Step reverse transcriptase real time PCR for the detection SYT-SSX transcript is feasible as an aid in confirming the diagnosis of synovial sarcoma.
format	Article
author	A.Talib, Norlelawati Goon, Mohd Danial Harun, Nora Othman, Nadia Kamaruzaman, Zatur Rawihah Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin
author_facet	A.Talib, Norlelawati Goon, Mohd Danial Harun, Nora Othman, Nadia Kamaruzaman, Zatur Rawihah Abdullah, Nor Zamzila Muhammad, Siti Aesah @ Naznin
author_sort	A.Talib, Norlelawati
title	Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR
title_short	Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR
title_full	Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR
title_fullStr	Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR
title_full_unstemmed	Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR
title_sort	detection of syt-ssx mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time pcr
publisher	College of Pathologists, Academy of Medicine Malaysia
publishDate	2016
url	http://irep.iium.edu.my/50533/ http://irep.iium.edu.my/50533/ http://irep.iium.edu.my/50533/1/detection-of-SYT-SSX.pdf http://irep.iium.edu.my/50533/4/50533_Detection_of_SYT-SSX_mutant_transcripts_in_formalin-fixed_SCOPUS.pdf
first_indexed	2023-09-18T21:11:25Z
last_indexed	2023-09-18T21:11:25Z
_version_	1777411277791952896

Detection of SYT-SSX mutant transcripts in formalin-fixed paraffin-embedded sarcoma tissues using one-step reverse transcriptase real-time PCR

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