Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae

The objective of this preliminary experiment was to test the hypothesis that coculturing commercialised ragi tapai with S. cerevisiae might have improved the ethanol production and reduce the accumulation of inhibitory concentration of reducing sugar and this would enhance the amylolytic activity....

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Main Authors: Azmi, Azlin Suhaida, Hasan, Masitah, Mel, Maizirwan, Ngoh, Cheng
Format: Article
Language:English
Published: The Italian Association of Chemical Engineering 2009
Subjects:
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spelling iium-50012017-06-14T01:19:40Z http://irep.iium.edu.my/5001/ Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae Azmi, Azlin Suhaida Hasan, Masitah Mel, Maizirwan Ngoh, Cheng TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol The objective of this preliminary experiment was to test the hypothesis that coculturing commercialised ragi tapai with S. cerevisiae might have improved the ethanol production and reduce the accumulation of inhibitory concentration of reducing sugar and this would enhance the amylolytic activity. Co culture of a commercialised ragi tapai with S cerevisiae using unhydrolysed raw starch in single step-fermentation produced a high ethanol concentration. A 35.26 giL of ethanol was produced when the starch was inoculated with ragi tapai and cocultured it with Scerevisiae 1 h later. This was 48% higher than the yield obtained when the starch was inoculated with ragi tapai only (23.79 giL). The glucose concentration was maintained at low concentration in the coculture medium compared to the medium with ragi tapai only. However, stachyose concentration profile was higher in cocultured medium as compared to the one with ragi tapai only. In conclusion, coculture ragi tapai with S cerevisiae enhanced the ethanol production and prevented the inhibitory effect of reducing sugars on amylase activity. The Italian Association of Chemical Engineering 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5001/1/Chem_Eng_Transaction_%28Azlin-2009%29.pdf Azmi, Azlin Suhaida and Hasan, Masitah and Mel, Maizirwan and Ngoh, Cheng (2009) Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae. Chemical Engineering Transactions, 18. pp. 557-562. ISSN 1974-9791 http://www.aidic.it/cet/09/18/programma.html 10.33031CET0918090
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP155 Chemical engineering
TP248.13 Biotechnology
TP315 Fuel
TP500 Fermentation industries. Beverages. Alcohol
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
TP315 Fuel
TP500 Fermentation industries. Beverages. Alcohol
Azmi, Azlin Suhaida
Hasan, Masitah
Mel, Maizirwan
Ngoh, Cheng
Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae
description The objective of this preliminary experiment was to test the hypothesis that coculturing commercialised ragi tapai with S. cerevisiae might have improved the ethanol production and reduce the accumulation of inhibitory concentration of reducing sugar and this would enhance the amylolytic activity. Co culture of a commercialised ragi tapai with S cerevisiae using unhydrolysed raw starch in single step-fermentation produced a high ethanol concentration. A 35.26 giL of ethanol was produced when the starch was inoculated with ragi tapai and cocultured it with Scerevisiae 1 h later. This was 48% higher than the yield obtained when the starch was inoculated with ragi tapai only (23.79 giL). The glucose concentration was maintained at low concentration in the coculture medium compared to the medium with ragi tapai only. However, stachyose concentration profile was higher in cocultured medium as compared to the one with ragi tapai only. In conclusion, coculture ragi tapai with S cerevisiae enhanced the ethanol production and prevented the inhibitory effect of reducing sugars on amylase activity.
format Article
author Azmi, Azlin Suhaida
Hasan, Masitah
Mel, Maizirwan
Ngoh, Cheng
author_facet Azmi, Azlin Suhaida
Hasan, Masitah
Mel, Maizirwan
Ngoh, Cheng
author_sort Azmi, Azlin Suhaida
title Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae
title_short Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae
title_full Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae
title_fullStr Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae
title_full_unstemmed Single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae
title_sort single-step bioconversion of starch to bioethanol by the coculture of ragi tapai and saccharomyces cerevisiae
publisher The Italian Association of Chemical Engineering
publishDate 2009
url http://irep.iium.edu.my/5001/
http://irep.iium.edu.my/5001/
http://irep.iium.edu.my/5001/
http://irep.iium.edu.my/5001/1/Chem_Eng_Transaction_%28Azlin-2009%29.pdf
first_indexed 2023-09-18T20:13:27Z
last_indexed 2023-09-18T20:13:27Z
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