Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood

The techniques of isolation deoxyribonucleic acid (DNA), a crucial step in the process of DNA analysis has progressed rapidly. The manual techniques for DNA purification have been gradually replaced by automation and purification kits. Although expensive, these commercialized techniques are prefer...

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Main Authors:	Mohammad, Salina, Abdullah, Nor Zamzila, Ku Zaifah, Norsidah, Farid Wajidi, Mustafa Fadzil, A. Talib, Norlelawati
Format:	Article
Language:	English
Published:	Malaysian Society of Applied Biology 2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/4999/ http://irep.iium.edu.my/4999/ http://irep.iium.edu.my/4999/1/06_SalinaM.pdf

id	iium-4999
recordtype	eprints
spelling	iium-49992017-06-15T06:38:49Z http://irep.iium.edu.my/4999/ Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood Mohammad, Salina Abdullah, Nor Zamzila Ku Zaifah, Norsidah Farid Wajidi, Mustafa Fadzil A. Talib, Norlelawati Q Science (General) The techniques of isolation deoxyribonucleic acid (DNA), a crucial step in the process of DNA analysis has progressed rapidly. The manual techniques for DNA purification have been gradually replaced by automation and purification kits. Although expensive, these commercialized techniques are prefered as they are superior in extracting DNA from small volumes of samples. These methods are also safer and rapid. The purpose of this study was to adopt and optimize the manual saltingout procedure for the purification of DNA from small volumes of blood, and to compare the cost of performing the technique to that of the Mini QIAmp DNA extraction kit (Qiagen) and fully automated technique Magtration 12C, Precision). Apart from being relatively safe and cheaper, we found that the optimized salting-out procedure was able to extract DNA that is comparable in quantity and quality from small volumes of blood. Malaysian Society of Applied Biology 2010-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4999/1/06_SalinaM.pdf Mohammad, Salina and Abdullah, Nor Zamzila and Ku Zaifah, Norsidah and Farid Wajidi, Mustafa Fadzil and A. Talib, Norlelawati (2010) Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood. Malaysian Applied Biology, 39 (1). pp. 43-48. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=174volume-391-june-2010&catid=58:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohammad, Salina Abdullah, Nor Zamzila Ku Zaifah, Norsidah Farid Wajidi, Mustafa Fadzil A. Talib, Norlelawati Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood
description	The techniques of isolation deoxyribonucleic acid (DNA), a crucial step in the process of DNA analysis has progressed rapidly. The manual techniques for DNA purification have been gradually replaced by automation and purification kits. Although expensive, these commercialized techniques are prefered as they are superior in extracting DNA from small volumes of samples. These methods are also safer and rapid. The purpose of this study was to adopt and optimize the manual saltingout procedure for the purification of DNA from small volumes of blood, and to compare the cost of performing the technique to that of the Mini QIAmp DNA extraction kit (Qiagen) and fully automated technique Magtration 12C, Precision). Apart from being relatively safe and cheaper, we found that the optimized salting-out procedure was able to extract DNA that is comparable in quantity and quality from small volumes of blood.
format	Article
author	Mohammad, Salina Abdullah, Nor Zamzila Ku Zaifah, Norsidah Farid Wajidi, Mustafa Fadzil A. Talib, Norlelawati
author_facet	Mohammad, Salina Abdullah, Nor Zamzila Ku Zaifah, Norsidah Farid Wajidi, Mustafa Fadzil A. Talib, Norlelawati
author_sort	Mohammad, Salina
title	Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood
title_short	Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood
title_full	Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood
title_fullStr	Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood
title_full_unstemmed	Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood
title_sort	comparison between manual salting-out and commercialized dna extraction from small volume of peripheral blood
publisher	Malaysian Society of Applied Biology
publishDate	2010
url	http://irep.iium.edu.my/4999/ http://irep.iium.edu.my/4999/ http://irep.iium.edu.my/4999/1/06_SalinaM.pdf
first_indexed	2023-09-18T20:13:27Z
last_indexed	2023-09-18T20:13:27Z
_version_	1777407630626521088

Comparison between manual salting-out and commercialized DNA extraction from small volume of peripheral blood

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