Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis

Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis

Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have...

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Main Authors: Barber, Carmel M., Ahmad Rusmili, Muhamad Rusdi, Hodgson, Wayne C.
Format: Article
Language:English
English
English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2016
Subjects:
QL Zoology
RM300 Drugs and their action
Online Access:http://irep.iium.edu.my/49966/
http://irep.iium.edu.my/49966/
http://irep.iium.edu.my/49966/
http://irep.iium.edu.my/49966/1/Carmel_2016_toxins-08-00058.pdf
http://irep.iium.edu.my/49966/4/49966-Isolation_and_pharmacological_characterization_SCOPUS.pdf
http://irep.iium.edu.my/49966/5/49966-Isolation_and_pharmacological_characterization_WOS.pdf
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spelling iium-499662017-01-19T06:20:27Z http://irep.iium.edu.my/49966/ Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis Barber, Carmel M. Ahmad Rusmili, Muhamad Rusdi Hodgson, Wayne C. QL Zoology RM300 Drugs and their action Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1–1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA2 value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins. Multidisciplinary Digital Publishing Institute (MDPI) 2016-02-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49966/1/Carmel_2016_toxins-08-00058.pdf application/pdf en http://irep.iium.edu.my/49966/4/49966-Isolation_and_pharmacological_characterization_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/49966/5/49966-Isolation_and_pharmacological_characterization_WOS.pdf Barber, Carmel M. and Ahmad Rusmili, Muhamad Rusdi and Hodgson, Wayne C. (2016) Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis. Toxins , 8 (3). pp. 1-12. ISSN 2072-6651 http://www.mdpi.com/2072-6651/8/3/58 10.3390/toxins8030058
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic QL Zoology
RM300 Drugs and their action
spellingShingle QL Zoology
RM300 Drugs and their action
Barber, Carmel M.
Ahmad Rusmili, Muhamad Rusdi
Hodgson, Wayne C.
Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis
description Taipans (Oxyuranus spp.) are elapids with highly potent venoms containing presynaptic (β) and postsynaptic (α) neurotoxins. O. temporalis (Western Desert taipan), a newly discovered member of this genus, has been shown to possess venom which displays marked in vitro neurotoxicity. No components have been isolated from this venom. We describe the characterization of α-elapitoxin-Ot1a (α-EPTX-Ot1a; 6712 Da), a short-chain postsynaptic neurotoxin, which accounts for approximately 30% of O. temporalis venom. α-Elapitoxin-Ot1a (0.1–1 µM) produced concentration-dependent inhibition of indirect-twitches, and abolished contractile responses to exogenous acetylcholine and carbachol, in the chick biventer cervicis nerve-muscle preparation. The inhibition of indirect twitches by α-elapitoxin-Ot1a (1 µM) was not reversed by washing the tissue. Prior addition of taipan antivenom (10 U/mL) delayed the neurotoxic effects of α-elapitoxin-Ot1a (1 µM) and markedly attenuated the neurotoxic effects of α-elapitoxin-Ot1a (0.1 µM). α-Elapitoxin-Ot1a displayed pseudo-irreversible antagonism of concentration-response curves to carbachol with a pA2 value of 8.02 ± 0.05. De novo sequencing revealed the main sequence of the short-chain postsynaptic neurotoxin (i.e., α-elapitoxin-Ot1a) as well as three other isoforms found in O. temporalis venom. α-Elapitoxin-Ot1a shows high sequence similarity (i.e., >87%) with other taipan short-chain postsynaptic neurotoxins.
format Article
author Barber, Carmel M.
Ahmad Rusmili, Muhamad Rusdi
Hodgson, Wayne C.
author_facet Barber, Carmel M.
Ahmad Rusmili, Muhamad Rusdi
Hodgson, Wayne C.
author_sort Barber, Carmel M.
title Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis
title_short Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis
title_full Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis
title_fullStr Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis
title_full_unstemmed Isolation and pharmacological characterization of α-Elapitoxin-Ot1a, a short-chain postsynaptic neurotoxin from the venom of the Western Desert Taipan, Oxyuranus temporalis
title_sort isolation and pharmacological characterization of α-elapitoxin-ot1a, a short-chain postsynaptic neurotoxin from the venom of the western desert taipan, oxyuranus temporalis
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publishDate 2016
url http://irep.iium.edu.my/49966/
http://irep.iium.edu.my/49966/
http://irep.iium.edu.my/49966/
http://irep.iium.edu.my/49966/1/Carmel_2016_toxins-08-00058.pdf
http://irep.iium.edu.my/49966/4/49966-Isolation_and_pharmacological_characterization_SCOPUS.pdf
http://irep.iium.edu.my/49966/5/49966-Isolation_and_pharmacological_characterization_WOS.pdf
first_indexed 2023-09-18T21:10:37Z
last_indexed 2023-09-18T21:10:37Z
_version_ 1777411227186626560

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