Isolation and identification of halophilic bacteria producing halotolerant protease

Halotolerant proteases are known as one of the important groups of enzymes that have been used widely in various industries. However, high production cost of proteases in term of energy used for sterilization and high risk of microbial contamination during fermentation become the obstacles during up...

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Main Authors:	Abd Samad, Nadiah Syuhada, Amid, Azura, Jimat, Dzun Noraini, Ab. Shukor, Nurul Aqilah
Format:	Article
Language:	English
Published:	Razi Publishing 2017
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/49851/ http://irep.iium.edu.my/49851/ http://irep.iium.edu.my/49851/5/49851_.%20Isolation%20And%20Identification.pdf

id	iium-49851
recordtype	eprints
spelling	iium-498512017-05-24T08:09:39Z http://irep.iium.edu.my/49851/ Isolation and identification of halophilic bacteria producing halotolerant protease Abd Samad, Nadiah Syuhada Amid, Azura Jimat, Dzun Noraini Ab. Shukor, Nurul Aqilah TP248.13 Biotechnology Halotolerant proteases are known as one of the important groups of enzymes that have been used widely in various industries. However, high production cost of proteases in term of energy used for sterilization and high risk of microbial contamination during fermentation become the obstacles during upstream processing. Moreover, proteases that capable to withstand with harsh conditions of salinity, temperature and pH are required in industry. Therefore, this study presents newly isolated halophilic bacteria producing halotolerant proteases. Halophilic bacteria were isolated from fermented fish sauce. Screening of the bacteria producing halotolerant protease enzymes was carried out by using skim milk salt agar containing 10% NaCl at pH 7 and incubated under aerobic condition at 37oC for 2 days. The selected isolates were identified based on their morphology followed by 16S rDNA gene sequence analysis. 40 colonies of halophiles bacteria were isolated, however, only 20 of them showing proteolytic activity. All of 20 isolates are motile and gram positive bacteria. From 20 isolates, only 6 were chosen for further analysis. B7 showed the highest proteolytic activity compared with others. Results of 16S rDNA gene sequence analysis showed 98% homology to Bacillus amyloliquefaciens subsp. plantarum strain FZB42. Therefore, B7 is identified as Bacillus amyloliquefaciens strain B7. Razi Publishing 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49851/5/49851_.%20Isolation%20And%20Identification.pdf Abd Samad, Nadiah Syuhada and Amid, Azura and Jimat, Dzun Noraini and Ab. Shukor, Nurul Aqilah (2017) Isolation and identification of halophilic bacteria producing halotolerant protease. Galeri Warisan Sains, 1 (1). 07-09. ISSN 2455-0983 http://www.razipublishing.com/archives/GWS/2017-issue1/1.%20Identification%20Of%20Potential%20Indigenous%20Microbe%20From%20Local%20Fermented.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abd Samad, Nadiah Syuhada Amid, Azura Jimat, Dzun Noraini Ab. Shukor, Nurul Aqilah Isolation and identification of halophilic bacteria producing halotolerant protease
description	Halotolerant proteases are known as one of the important groups of enzymes that have been used widely in various industries. However, high production cost of proteases in term of energy used for sterilization and high risk of microbial contamination during fermentation become the obstacles during upstream processing. Moreover, proteases that capable to withstand with harsh conditions of salinity, temperature and pH are required in industry. Therefore, this study presents newly isolated halophilic bacteria producing halotolerant proteases. Halophilic bacteria were isolated from fermented fish sauce. Screening of the bacteria producing halotolerant protease enzymes was carried out by using skim milk salt agar containing 10% NaCl at pH 7 and incubated under aerobic condition at 37oC for 2 days. The selected isolates were identified based on their morphology followed by 16S rDNA gene sequence analysis. 40 colonies of halophiles bacteria were isolated, however, only 20 of them showing proteolytic activity. All of 20 isolates are motile and gram positive bacteria. From 20 isolates, only 6 were chosen for further analysis. B7 showed the highest proteolytic activity compared with others. Results of 16S rDNA gene sequence analysis showed 98% homology to Bacillus amyloliquefaciens subsp. plantarum strain FZB42. Therefore, B7 is identified as Bacillus amyloliquefaciens strain B7.
format	Article
author	Abd Samad, Nadiah Syuhada Amid, Azura Jimat, Dzun Noraini Ab. Shukor, Nurul Aqilah
author_facet	Abd Samad, Nadiah Syuhada Amid, Azura Jimat, Dzun Noraini Ab. Shukor, Nurul Aqilah
author_sort	Abd Samad, Nadiah Syuhada
title	Isolation and identification of halophilic bacteria producing halotolerant protease
title_short	Isolation and identification of halophilic bacteria producing halotolerant protease
title_full	Isolation and identification of halophilic bacteria producing halotolerant protease
title_fullStr	Isolation and identification of halophilic bacteria producing halotolerant protease
title_full_unstemmed	Isolation and identification of halophilic bacteria producing halotolerant protease
title_sort	isolation and identification of halophilic bacteria producing halotolerant protease
publisher	Razi Publishing
publishDate	2017
url	http://irep.iium.edu.my/49851/ http://irep.iium.edu.my/49851/ http://irep.iium.edu.my/49851/5/49851_.%20Isolation%20And%20Identification.pdf
first_indexed	2023-09-18T21:10:26Z
last_indexed	2023-09-18T21:10:26Z
_version_	1777411215830548480

Isolation and identification of halophilic bacteria producing halotolerant protease

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