Bromelain enzyme assay using Casen as substrate
This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's rea...
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| Format: | Book Chapter |
| Language: | English |
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IIUM Press
2016
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| Online Access: | http://irep.iium.edu.my/49849/ http://irep.iium.edu.my/49849/ http://irep.iium.edu.my/49849/1/49849_Bromelain_enzyme_assay_using_casein.pdf |
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iium-49849 |
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eprints |
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iium-498492017-02-21T02:05:49Z http://irep.iium.edu.my/49849/ Bromelain enzyme assay using Casen as substrate Amid, Azura Jamaluddin, Mohd Jamil Aizat TP248.13 Biotechnology This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's reagent primarily reacts with free tyrosine to produce a blue colored chromophore, which is quantifiable and measured as an absorbance value on the spectrophotometer at 660 nm absorbance. The more tyrosine that is released from the casein, the more chromophores are generated and the stronger the activity of the protease. IIUM Press 2016 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/49849/1/49849_Bromelain_enzyme_assay_using_casein.pdf Amid, Azura and Jamaluddin, Mohd Jamil Aizat (2016) Bromelain enzyme assay using Casen as substrate. In: Experimental methods in modern biotechnology Volume 2. IIUM Press Series of Textbook from the Islamic Perspectives, 2 . IIUM Press, Kuala Lumpur, pp. 100-150. ISBN 978-967-418-384-4 http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Amid, Azura Jamaluddin, Mohd Jamil Aizat Bromelain enzyme assay using Casen as substrate |
| description |
This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's reagent primarily reacts with free tyrosine to produce a blue colored chromophore, which is quantifiable and measured as an absorbance value on the spectrophotometer at 660 nm absorbance. The more tyrosine that is released from the casein, the more chromophores are generated and the stronger the activity of the protease. |
| format |
Book Chapter |
| author |
Amid, Azura Jamaluddin, Mohd Jamil Aizat |
| author_facet |
Amid, Azura Jamaluddin, Mohd Jamil Aizat |
| author_sort |
Amid, Azura |
| title |
Bromelain enzyme assay using Casen as substrate |
| title_short |
Bromelain enzyme assay using Casen as substrate |
| title_full |
Bromelain enzyme assay using Casen as substrate |
| title_fullStr |
Bromelain enzyme assay using Casen as substrate |
| title_full_unstemmed |
Bromelain enzyme assay using Casen as substrate |
| title_sort |
bromelain enzyme assay using casen as substrate |
| publisher |
IIUM Press |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/49849/ http://irep.iium.edu.my/49849/ http://irep.iium.edu.my/49849/1/49849_Bromelain_enzyme_assay_using_casein.pdf |
| first_indexed |
2023-09-18T21:10:26Z |
| last_indexed |
2023-09-18T21:10:26Z |
| _version_ |
1777411215697379328 |