Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line

The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta pote...

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Main Authors:	Doolaanea, Abd Almonem, Mansor, Nur 'Izzati, Mohd Nor, Nurul Hafizah, Mohamed, Farahidah
Format:	Article
Language:	English English
Published:	Informa UK Ltd 2014
Subjects:	RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/1/49758_-_Cellular_uptake_of_Nigella_sativa_oil-PLGA_microparticle_by_PC-12_cell_line.pdf http://irep.iium.edu.my/49758/4/49758_Cellular%20uptake%20of%20Nigella%20sativa%20oil_SCOPUS.pdf

id	iium-49758
recordtype	eprints
spelling	iium-497582018-01-05T03:27:21Z http://irep.iium.edu.my/49758/ Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah RM Therapeutics. Pharmacology RS Pharmacy and materia medica The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potential and morphology of the particle. The results revealed varying efficiencies of the cell uptake by PC-12 cells, which may be partially attributed to the surface hydrophobicity of the microparticles. Interestingly, the uptake efficiency of PC-12 cells was higher with the more hydrophilic microparticle. NSO microparticle showed evidence of being preferably internalised by mitotic cells. Tween 80 microparticle showed the highest cell uptake efficiency with a concentration-dependent pattern suggesting its use as uptake enhancer for nonscavenging cells. In conclusion, PC-12 cells can take up NSO-PLGA microparticle which may have potential in the treatment of neurodegenerative disease. Informa UK Ltd 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49758/1/49758_-_Cellular_uptake_of_Nigella_sativa_oil-PLGA_microparticle_by_PC-12_cell_line.pdf application/pdf en http://irep.iium.edu.my/49758/4/49758_Cellular%20uptake%20of%20Nigella%20sativa%20oil_SCOPUS.pdf Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2014) Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line. Journal of Microencapsulation, 31 (6). pp. 600-608. ISSN 0265-2048 E-ISSN 1464-5246 http://www.tandfonline.com/toc/imnc20/current DOI: 10.3109/02652048.2014.898709
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics. Pharmacology RS Pharmacy and materia medica Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line
description	The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potential and morphology of the particle. The results revealed varying efficiencies of the cell uptake by PC-12 cells, which may be partially attributed to the surface hydrophobicity of the microparticles. Interestingly, the uptake efficiency of PC-12 cells was higher with the more hydrophilic microparticle. NSO microparticle showed evidence of being preferably internalised by mitotic cells. Tween 80 microparticle showed the highest cell uptake efficiency with a concentration-dependent pattern suggesting its use as uptake enhancer for nonscavenging cells. In conclusion, PC-12 cells can take up NSO-PLGA microparticle which may have potential in the treatment of neurodegenerative disease.
format	Article
author	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line
title_short	Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line
title_full	Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line
title_fullStr	Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line
title_full_unstemmed	Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line
title_sort	cellular uptake of nigella sativa oil-plga microparticle by pc12 cell line
publisher	Informa UK Ltd
publishDate	2014
url	http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/1/49758_-_Cellular_uptake_of_Nigella_sativa_oil-PLGA_microparticle_by_PC-12_cell_line.pdf http://irep.iium.edu.my/49758/4/49758_Cellular%20uptake%20of%20Nigella%20sativa%20oil_SCOPUS.pdf
first_indexed	2023-09-18T21:10:19Z
last_indexed	2023-09-18T21:10:19Z
_version_	1777411208845983744

Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line

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