Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line
The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta pote...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Informa UK Ltd
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/ http://irep.iium.edu.my/49758/1/49758_-_Cellular_uptake_of_Nigella_sativa_oil-PLGA_microparticle_by_PC-12_cell_line.pdf http://irep.iium.edu.my/49758/4/49758_Cellular%20uptake%20of%20Nigella%20sativa%20oil_SCOPUS.pdf |
| Summary: | The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA
microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 &
Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the
size, zeta potential and morphology of the particle. The results revealed varying efficiencies of
the cell uptake by PC-12 cells, which may be partially attributed to the surface hydrophobicity
of the microparticles. Interestingly, the uptake efficiency of PC-12 cells was higher with the
more hydrophilic microparticle. NSO microparticle showed evidence of being preferably
internalised by mitotic cells. Tween 80 microparticle showed the highest cell uptake efficiency
with a concentration-dependent pattern suggesting its use as uptake enhancer for nonscavenging
cells. In conclusion, PC-12 cells can take up NSO-PLGA microparticle which may
have potential in the treatment of neurodegenerative disease. |
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