Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract

Ethnopharmacological relevance ‘Pegaga’ is a traditional Malay remedy for a wide range of complaints. Among the 'pegaga’, Centella asiatica has been used as a remedy for diabetes mellitus. Thus, we decided to validate this claim by evaluating the in vivo antidiabetic property of C. asiatica...

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Main Authors:	Maulidiani, H, Abas, Faridah, Khatib, Alfi, Perumal, Vikneswari, Suppaiah, V, Ismail, A, Hamid, M, Shaari, Khozirah, Lajis, NH
Format:	Article
Language:	English
Published:	Elsevier Ireland Ltd 2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/49628/ http://irep.iium.edu.my/49628/ http://irep.iium.edu.my/49628/ http://irep.iium.edu.my/49628/1/maulidiani_et_al_2016_JE.pdf

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recordtype	eprints
spelling	iium-496282017-01-04T04:07:33Z http://irep.iium.edu.my/49628/ Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract Maulidiani, H Abas, Faridah Khatib, Alfi Perumal, Vikneswari Suppaiah, V Ismail, A Hamid, M Shaari, Khozirah Lajis, NH QD Chemistry Ethnopharmacological relevance ‘Pegaga’ is a traditional Malay remedy for a wide range of complaints. Among the 'pegaga’, Centella asiatica has been used as a remedy for diabetes mellitus. Thus, we decided to validate this claim by evaluating the in vivo antidiabetic property of C. asiatica (CA) on T2DM rat model using the holistic 1H NMR-based metabolomics approach. Method In this study, an obese diabetic (mimic of T2DM condition) animal model was developed using Sprague–Dawley rats fed with a high-fat diet and induced into diabetic condition by the treatment of a low dose of streptozotocin (STZ). The effect of C. asiatica extract on the experimental animals was followed based on the changes observed in the urinary and serum metabolites, measured by 1H NMR of urine and blood samples collected over the test period. Results A long-term treatment of obese diabetic rats with CA extract could reverse the glucose and lipid levels, as well as the tricarboxylic acid cycle and amino acid metabolic disorders, back towards normal states. Biochemical analysis also showed an increase of insulin production in diabetic rats upon treatment of CA extract. Conclusion This study has provided evidence that clearly supported the traditional use of CA as a remedy for diabetes. NMR-based metabolomics was successfully applied to show that CA produced both anti-hyperglycemic and anti-hyperlipidemic effects on a rat model. In addition to increasing the insulin secretion, the CA extract also ameliorates the metabolic pathways affected in the induced diabectic rats. This study further revealed the potential usage of CA extract in managing diabetes mellitus and the results of this work may contribute towards the further understanding of the underlying molecular mechanism of this herbal remedy Elsevier Ireland Ltd 2016-03-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49628/1/maulidiani_et_al_2016_JE.pdf Maulidiani, H and Abas, Faridah and Khatib, Alfi and Perumal, Vikneswari and Suppaiah, V and Ismail, A and Hamid, M and Shaari, Khozirah and Lajis, NH (2016) Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract. Journal of Ethnopharmacology, 180. pp. 60-69. ISSN 0378-8741 http://www.sciencedirect.com/science/article/pii/S0378874116300034 10.1016/j.jep.2016.01.001
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Maulidiani, H Abas, Faridah Khatib, Alfi Perumal, Vikneswari Suppaiah, V Ismail, A Hamid, M Shaari, Khozirah Lajis, NH Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract
description	Ethnopharmacological relevance ‘Pegaga’ is a traditional Malay remedy for a wide range of complaints. Among the 'pegaga’, Centella asiatica has been used as a remedy for diabetes mellitus. Thus, we decided to validate this claim by evaluating the in vivo antidiabetic property of C. asiatica (CA) on T2DM rat model using the holistic 1H NMR-based metabolomics approach. Method In this study, an obese diabetic (mimic of T2DM condition) animal model was developed using Sprague–Dawley rats fed with a high-fat diet and induced into diabetic condition by the treatment of a low dose of streptozotocin (STZ). The effect of C. asiatica extract on the experimental animals was followed based on the changes observed in the urinary and serum metabolites, measured by 1H NMR of urine and blood samples collected over the test period. Results A long-term treatment of obese diabetic rats with CA extract could reverse the glucose and lipid levels, as well as the tricarboxylic acid cycle and amino acid metabolic disorders, back towards normal states. Biochemical analysis also showed an increase of insulin production in diabetic rats upon treatment of CA extract. Conclusion This study has provided evidence that clearly supported the traditional use of CA as a remedy for diabetes. NMR-based metabolomics was successfully applied to show that CA produced both anti-hyperglycemic and anti-hyperlipidemic effects on a rat model. In addition to increasing the insulin secretion, the CA extract also ameliorates the metabolic pathways affected in the induced diabectic rats. This study further revealed the potential usage of CA extract in managing diabetes mellitus and the results of this work may contribute towards the further understanding of the underlying molecular mechanism of this herbal remedy
format	Article
author	Maulidiani, H Abas, Faridah Khatib, Alfi Perumal, Vikneswari Suppaiah, V Ismail, A Hamid, M Shaari, Khozirah Lajis, NH
author_facet	Maulidiani, H Abas, Faridah Khatib, Alfi Perumal, Vikneswari Suppaiah, V Ismail, A Hamid, M Shaari, Khozirah Lajis, NH
author_sort	Maulidiani, H
title	Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract
title_short	Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract
title_full	Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract
title_fullStr	Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract
title_full_unstemmed	Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract
title_sort	metabolic alteration in obese diabetes rats upon treatment with centella asiatica extract
publisher	Elsevier Ireland Ltd
publishDate	2016
url	http://irep.iium.edu.my/49628/ http://irep.iium.edu.my/49628/ http://irep.iium.edu.my/49628/ http://irep.iium.edu.my/49628/1/maulidiani_et_al_2016_JE.pdf
first_indexed	2023-09-18T21:10:09Z
last_indexed	2023-09-18T21:10:09Z
_version_	1777411197870538752

Metabolic alteration in obese diabetes rats upon treatment with Centella asiatica extract

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