Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach

Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range ofmetabolites, including...

Full description

Bibliographic Details
Main Authors:	Javadi, Neda, Abas, Faridah, Mediani, Ahmed, Abdul Hamid, Azizah, Khatib, Alfi, Simoh, Sanimah, Shaari, Khozirah
Format:	Article
Language:	English
Published:	Elsevier 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/1/Neda_et_al._2015_JFDA.pdf

id	iium-49626
recordtype	eprints
spelling	iium-496262016-02-23T06:55:45Z http://irep.iium.edu.my/49626/ Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach Javadi, Neda Abas, Faridah Mediani, Ahmed Abdul Hamid, Azizah Khatib, Alfi Simoh, Sanimah Shaari, Khozirah Q Science (General) Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range ofmetabolites, including glucosinolates, phenolics, amino acids, organic acids, and sugars. However, many of these metabolites are not stable and easily degraded or modified during storage. In order to investigate the metabolomics changes occurring during post-harvest storage, C. caudatus samples were subjected to seven different storage times (0 hours, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, and 12 hours) at room temperature. As the model experiment, the metabolites identified by gas chromatography-mass spectrometry (GC-MS) were correlated with a-glucosidase inhibitory activity analyzed with multivariate data analysis (MVDA) to find out the variation among samples and metabolites contributing to the activity. Orthogonal partial least squares (OPLS) analysis was applied to investigate the metabolomics changes. A profound chemical alteration, both in primary and secondary metabolites, was observed. The a-tocopherol, catechin, cyclohexen-1-carboxylic acid, benzoic acid, myo-inositol, stigmasterol, and lycopene compoundswere found to be the discriminatingmetabolites at early storage;however, sugars such as sucrose, a-D-galactopyranose, and turanose were detected, which was attributed to the discriminating metabolites for late storage. The result shows that the MVDA method is a promising technique to identify biomarker compounds relative to storage at different times Elsevier 2015-03-28 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49626/1/Neda_et_al._2015_JFDA.pdf Javadi, Neda and Abas, Faridah and Mediani, Ahmed and Abdul Hamid, Azizah and Khatib, Alfi and Simoh, Sanimah and Shaari, Khozirah (2015) Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach. Journal of Food and Drug Analysis, 23. pp. 433-441. ISSN 1021-9498 http://www.journals.elsevier.com/journal-of-food-and-drug-analysis/ 10.1016/j.jfda.2015.01.005
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Javadi, Neda Abas, Faridah Mediani, Ahmed Abdul Hamid, Azizah Khatib, Alfi Simoh, Sanimah Shaari, Khozirah Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach
description	Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range ofmetabolites, including glucosinolates, phenolics, amino acids, organic acids, and sugars. However, many of these metabolites are not stable and easily degraded or modified during storage. In order to investigate the metabolomics changes occurring during post-harvest storage, C. caudatus samples were subjected to seven different storage times (0 hours, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, and 12 hours) at room temperature. As the model experiment, the metabolites identified by gas chromatography-mass spectrometry (GC-MS) were correlated with a-glucosidase inhibitory activity analyzed with multivariate data analysis (MVDA) to find out the variation among samples and metabolites contributing to the activity. Orthogonal partial least squares (OPLS) analysis was applied to investigate the metabolomics changes. A profound chemical alteration, both in primary and secondary metabolites, was observed. The a-tocopherol, catechin, cyclohexen-1-carboxylic acid, benzoic acid, myo-inositol, stigmasterol, and lycopene compoundswere found to be the discriminatingmetabolites at early storage;however, sugars such as sucrose, a-D-galactopyranose, and turanose were detected, which was attributed to the discriminating metabolites for late storage. The result shows that the MVDA method is a promising technique to identify biomarker compounds relative to storage at different times
format	Article
author	Javadi, Neda Abas, Faridah Mediani, Ahmed Abdul Hamid, Azizah Khatib, Alfi Simoh, Sanimah Shaari, Khozirah
author_facet	Javadi, Neda Abas, Faridah Mediani, Ahmed Abdul Hamid, Azizah Khatib, Alfi Simoh, Sanimah Shaari, Khozirah
author_sort	Javadi, Neda
title	Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach
title_short	Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach
title_full	Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach
title_fullStr	Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach
title_full_unstemmed	Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach
title_sort	effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of cosmos caudatus leaves - gcms based metabolomics approach
publisher	Elsevier
publishDate	2015
url	http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/1/Neda_et_al._2015_JFDA.pdf
first_indexed	2023-09-18T21:10:08Z
last_indexed	2023-09-18T21:10:08Z
_version_	1777411197722689536

Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach

Similar Items