Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves - GCMS based metabolomics approach
Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range ofmetabolites, including...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/ http://irep.iium.edu.my/49626/1/Neda_et_al._2015_JFDA.pdf |
| Summary: | Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as
“Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat
several maladies. Its bioactive or nutritional constituents consist of a wide range ofmetabolites,
including glucosinolates, phenolics, amino acids, organic acids, and sugars. However, many of
these metabolites are not stable and easily degraded or modified during storage. In order to
investigate the metabolomics changes occurring during post-harvest storage, C. caudatus
samples were subjected to seven different storage times (0 hours, 2 hours, 4 hours, 6 hours, 8
hours, 10 hours, and 12 hours) at room temperature. As the model experiment, the metabolites
identified by gas chromatography-mass spectrometry (GC-MS) were correlated with a-glucosidase
inhibitory activity analyzed with multivariate data analysis (MVDA) to find out the
variation among samples and metabolites contributing to the activity. Orthogonal partial least
squares (OPLS) analysis was applied to investigate the metabolomics changes. A profound
chemical alteration, both in primary and secondary metabolites, was observed. The a-tocopherol,
catechin, cyclohexen-1-carboxylic acid, benzoic acid, myo-inositol, stigmasterol, and
lycopene compoundswere found to be the discriminatingmetabolites at early storage;however,
sugars such as sucrose, a-D-galactopyranose, and turanose were detected, which was attributed
to the discriminating metabolites for late storage. The result shows that the MVDA method is a
promising technique to identify biomarker compounds relative to storage at different times |
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