Spectrophotometric determination of gentamicin loaded PLGA microparticles and method validation via ninhydrin-gentamicin complex as a rapid quantification approach
The purpose of this study was to provide a quantification method with rapid, sensitive, reproducible and cost effective for gentamicin in the form of ninhydrin-gentamicin complex. The utilization of spectrophotometric module to validate the method development for gentamicin loaded microparticles w...
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| Format: | Article |
| Language: | English English |
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Journal of Applied Pharmaceutical Science (http://www.japsonline.com)
2016
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| Online Access: | http://irep.iium.edu.my/49474/ http://irep.iium.edu.my/49474/ http://irep.iium.edu.my/49474/1/19-1446706340.pdf http://irep.iium.edu.my/49474/4/49474-Spectrophotometric%20determination%20of%20gentamicin%20loaded%20PLGA%20_SCOPUS.pdf |
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iium-49474 |
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eprints |
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iium-494742019-01-04T08:20:14Z http://irep.iium.edu.my/49474/ Spectrophotometric determination of gentamicin loaded PLGA microparticles and method validation via ninhydrin-gentamicin complex as a rapid quantification approach Harun @ Ismail, Ahmad Fahmi Mohamed, Farahidah Mohd Rosli, Luqman Muizzudin Mohd Shafri, Mohd Affendi Haris, Muhammad Salahuddin Adina, Anugerah Budipratama RM Therapeutics. Pharmacology The purpose of this study was to provide a quantification method with rapid, sensitive, reproducible and cost effective for gentamicin in the form of ninhydrin-gentamicin complex. The utilization of spectrophotometric module to validate the method development for gentamicin loaded microparticles was intended to provide alternative quantification method without undermining the sensitivity of the developed method. The microparticles fabrication process was proven to be suitable in encapsulating gentamicin by using poly(lactic-coglycolic acid) PLGA without compromising the efficacy of the antibiotic itself. The linearity of 6 known concentrations of ninhydrin-gentamicin complex was obtained with the R2 of 0.9998. The interday and intraday precisions were determined with the acceptance %RSD values of less than 2%. The highest %RSD value was 1.09% which suggested the method to be acceptably precise. The limit of detection (LOD) and limit of quantification (LOQ) values were recorded to be at 0.016 and 0.196 mg/mL respectively. The % recovery of 4 known concentrations indicated the accuracy of the method was high with the recovery range between 98.66% and 101.8%. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines. This quantification method was a promising approach to provide a rapid and cost effective in evaluating gentamicin concentration for in-vitro applications. Journal of Applied Pharmaceutical Science (http://www.japsonline.com) 2016-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49474/1/19-1446706340.pdf application/pdf en http://irep.iium.edu.my/49474/4/49474-Spectrophotometric%20determination%20of%20gentamicin%20loaded%20PLGA%20_SCOPUS.pdf Harun @ Ismail, Ahmad Fahmi and Mohamed, Farahidah and Mohd Rosli, Luqman Muizzudin and Mohd Shafri, Mohd Affendi and Haris, Muhammad Salahuddin and Adina, Anugerah Budipratama (2016) Spectrophotometric determination of gentamicin loaded PLGA microparticles and method validation via ninhydrin-gentamicin complex as a rapid quantification approach. Journal of Applied Pharmaceutical Science, 6 (1). pp. 7-14. ISSN 2231-3354 http://www.japsonline.com/admin/php/uploads/1741_pdf.pdf |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
RM Therapeutics. Pharmacology |
| spellingShingle |
RM Therapeutics. Pharmacology Harun @ Ismail, Ahmad Fahmi Mohamed, Farahidah Mohd Rosli, Luqman Muizzudin Mohd Shafri, Mohd Affendi Haris, Muhammad Salahuddin Adina, Anugerah Budipratama Spectrophotometric determination of gentamicin loaded PLGA microparticles and method validation via ninhydrin-gentamicin complex as a rapid quantification approach |
| description |
The purpose of this study was to provide a quantification method with rapid, sensitive, reproducible and cost
effective for gentamicin in the form of ninhydrin-gentamicin complex. The utilization of spectrophotometric
module to validate the method development for gentamicin loaded microparticles was intended to provide
alternative quantification method without undermining the sensitivity of the developed method. The
microparticles fabrication process was proven to be suitable in encapsulating gentamicin by using poly(lactic-coglycolic
acid) PLGA without compromising the efficacy of the antibiotic itself. The linearity of 6 known
concentrations of ninhydrin-gentamicin complex was obtained with the R2 of 0.9998. The interday and intraday
precisions were determined with the acceptance %RSD values of less than 2%. The highest %RSD value was
1.09% which suggested the method to be acceptably precise. The limit of detection (LOD) and limit of
quantification (LOQ) values were recorded to be at 0.016 and 0.196 mg/mL respectively. The % recovery of 4
known concentrations indicated the accuracy of the method was high with the recovery range between 98.66%
and 101.8%. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines. This
quantification method was a promising approach to provide a rapid and cost effective in evaluating gentamicin
concentration for in-vitro applications. |
| format |
Article |
| author |
Harun @ Ismail, Ahmad Fahmi Mohamed, Farahidah Mohd Rosli, Luqman Muizzudin Mohd Shafri, Mohd Affendi Haris, Muhammad Salahuddin Adina, Anugerah Budipratama |
| author_facet |
Harun @ Ismail, Ahmad Fahmi Mohamed, Farahidah Mohd Rosli, Luqman Muizzudin Mohd Shafri, Mohd Affendi Haris, Muhammad Salahuddin Adina, Anugerah Budipratama |
| author_sort |
Harun @ Ismail, Ahmad Fahmi |
| title |
Spectrophotometric determination of gentamicin loaded PLGA
microparticles and method validation via ninhydrin-gentamicin
complex as a rapid quantification approach |
| title_short |
Spectrophotometric determination of gentamicin loaded PLGA
microparticles and method validation via ninhydrin-gentamicin
complex as a rapid quantification approach |
| title_full |
Spectrophotometric determination of gentamicin loaded PLGA
microparticles and method validation via ninhydrin-gentamicin
complex as a rapid quantification approach |
| title_fullStr |
Spectrophotometric determination of gentamicin loaded PLGA
microparticles and method validation via ninhydrin-gentamicin
complex as a rapid quantification approach |
| title_full_unstemmed |
Spectrophotometric determination of gentamicin loaded PLGA
microparticles and method validation via ninhydrin-gentamicin
complex as a rapid quantification approach |
| title_sort |
spectrophotometric determination of gentamicin loaded plga
microparticles and method validation via ninhydrin-gentamicin
complex as a rapid quantification approach |
| publisher |
Journal of Applied Pharmaceutical Science (http://www.japsonline.com) |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/49474/ http://irep.iium.edu.my/49474/ http://irep.iium.edu.my/49474/1/19-1446706340.pdf http://irep.iium.edu.my/49474/4/49474-Spectrophotometric%20determination%20of%20gentamicin%20loaded%20PLGA%20_SCOPUS.pdf |
| first_indexed |
2023-09-18T21:09:56Z |
| last_indexed |
2023-09-18T21:09:56Z |
| _version_ |
1777411185076862976 |