Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats

Eurycoma longifolia is one of the plants used to enhanced male reproductive functions but little is known for its effect on the female reproductive system and functions. Therefore this work is performed to study the effects of standardized extract of Eurycoma longifolia, TAF 273, on oestrous cycle a...

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Main Authors:	Ahmad, Nur Amalina, Abdul Rahman, Suzanah, Chan, Kit-Lam, Mamat, Suhana, Abdul Samat, Nadia Hanis, Abdullah, Afif Raihan, Mohd Zahri, Syazana
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/49399/ http://irep.iium.edu.my/49399/1/49399_Short-Term%20Effects%20of%20TAF%20273.pdf http://irep.iium.edu.my/49399/2/49399_slide.pdf

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recordtype	eprints
spelling	iium-493992017-03-30T03:50:29Z http://irep.iium.edu.my/49399/ Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats Ahmad, Nur Amalina Abdul Rahman, Suzanah Chan, Kit-Lam Mamat, Suhana Abdul Samat, Nadia Hanis Abdullah, Afif Raihan Mohd Zahri, Syazana RM Therapeutics. Pharmacology RM300 Drugs and their action Eurycoma longifolia is one of the plants used to enhanced male reproductive functions but little is known for its effect on the female reproductive system and functions. Therefore this work is performed to study the effects of standardized extract of Eurycoma longifolia, TAF 273, on oestrous cycle and some reproductive functionality in rats. Female Sprague-dawley rats (7-8 weeks old) weighing between 160-230 g were used in this study. Vaginal smear of each rat was monitored daily for 2-week period (cover 3 cycles) in order to select females with normal cycle (4-5 days). Those with regular cycle were randomized into seven groups of 6 animals each. Rats in the treatment group received the extract orally at different doses (2.5, 5, 10, 25, 50 and 100 mg/kg body weight) for 5 days (one complete cycle) whereas the control group received an equal volume of distilled water in a similar manner. The oestrous cycle pattern of both control and treatment groups were monitored daily and recorded during the 5 days treatment and during the following 15 days after withdrawal of treatment. The body weight of the females was recorded before and after 5 days treatment. At the end of the experiments, blood samples were collected and the serum were analysed for oestrogen, progesterone and testosterone levels. Ovaries and uteri were removed, weighed and examined for histomorphological changes. The results showed that the administration of TAF 273 did not significantly alter the analyzed variables. Therefore, the data obtained indicate that Eurycoma longifolia standardized extract did not exhibit any detrimental effects on the oestrous cycle, ovarian histomorphology and female reproductive hormones. Keywords: Eurycoma longifolia, TAF 273, Oestrous Cycle, Reproductive Hormones, Ovary 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/49399/1/49399_Short-Term%20Effects%20of%20TAF%20273.pdf application/pdf en http://irep.iium.edu.my/49399/2/49399_slide.pdf Ahmad, Nur Amalina and Abdul Rahman, Suzanah and Chan, Kit-Lam and Mamat, Suhana and Abdul Samat, Nadia Hanis and Abdullah, Afif Raihan and Mohd Zahri, Syazana (2015) Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats. In: 5th International Conference on Advancement in Science and Technology (iCAST 2015), 10th-12th August 2015, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action Ahmad, Nur Amalina Abdul Rahman, Suzanah Chan, Kit-Lam Mamat, Suhana Abdul Samat, Nadia Hanis Abdullah, Afif Raihan Mohd Zahri, Syazana Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats
description	Eurycoma longifolia is one of the plants used to enhanced male reproductive functions but little is known for its effect on the female reproductive system and functions. Therefore this work is performed to study the effects of standardized extract of Eurycoma longifolia, TAF 273, on oestrous cycle and some reproductive functionality in rats. Female Sprague-dawley rats (7-8 weeks old) weighing between 160-230 g were used in this study. Vaginal smear of each rat was monitored daily for 2-week period (cover 3 cycles) in order to select females with normal cycle (4-5 days). Those with regular cycle were randomized into seven groups of 6 animals each. Rats in the treatment group received the extract orally at different doses (2.5, 5, 10, 25, 50 and 100 mg/kg body weight) for 5 days (one complete cycle) whereas the control group received an equal volume of distilled water in a similar manner. The oestrous cycle pattern of both control and treatment groups were monitored daily and recorded during the 5 days treatment and during the following 15 days after withdrawal of treatment. The body weight of the females was recorded before and after 5 days treatment. At the end of the experiments, blood samples were collected and the serum were analysed for oestrogen, progesterone and testosterone levels. Ovaries and uteri were removed, weighed and examined for histomorphological changes. The results showed that the administration of TAF 273 did not significantly alter the analyzed variables. Therefore, the data obtained indicate that Eurycoma longifolia standardized extract did not exhibit any detrimental effects on the oestrous cycle, ovarian histomorphology and female reproductive hormones. Keywords: Eurycoma longifolia, TAF 273, Oestrous Cycle, Reproductive Hormones, Ovary
format	Conference or Workshop Item
author	Ahmad, Nur Amalina Abdul Rahman, Suzanah Chan, Kit-Lam Mamat, Suhana Abdul Samat, Nadia Hanis Abdullah, Afif Raihan Mohd Zahri, Syazana
author_facet	Ahmad, Nur Amalina Abdul Rahman, Suzanah Chan, Kit-Lam Mamat, Suhana Abdul Samat, Nadia Hanis Abdullah, Afif Raihan Mohd Zahri, Syazana
author_sort	Ahmad, Nur Amalina
title	Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats
title_short	Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats
title_full	Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats
title_fullStr	Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats
title_full_unstemmed	Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats
title_sort	short-term effects of taf 273 on oestrous cycle and reproductive hormones of normal rats
publishDate	2015
url	http://irep.iium.edu.my/49399/ http://irep.iium.edu.my/49399/1/49399_Short-Term%20Effects%20of%20TAF%20273.pdf http://irep.iium.edu.my/49399/2/49399_slide.pdf
first_indexed	2023-09-18T21:09:51Z
last_indexed	2023-09-18T21:09:51Z
_version_	1777411179691376640

Short-term effects of TAF 273 on oestrous cycle and reproductive hormones of normal rats

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