Activation of mouse oocytes and in vitro embryo enhancing potential of Nigella Sativa
Objective: Aim of the present study is to optimize the development of the mouse embryo with addition of different concentrations of Nigella sativa extract in the culture. Methods: In this study, three different concentrations of Nigella sativa supplementation were used to assist in the enhancement...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/49392/ http://irep.iium.edu.my/49392/ http://irep.iium.edu.my/49392/14/ICAMS%202015%20Programme%20_complete.pdf |
| Summary: | Objective: Aim of the present study is to optimize the development of the mouse embryo with addition of different concentrations of Nigella sativa extract in the culture.
Methods: In this study, three different concentrations of Nigella sativa supplementation were used to assist in the enhancement of embryo development. G-1 media was used as a control (Group I). Groups II, III and IV were modified with the addition of Nigella sativa at low dose (5%), moderate dose (10%) and high dose (15%) respectively.
Results: Results showed an increase in the number of oocytes that successfully developed into morula and blastocyst after supplementation with three different concentrations of Nigella sativa in the embryo medium.
Conclusions: The outcome of the study indicated that Nigella sativa supplementation at a moderate concentration may help in the optimization of mouse embryo culture leading to an increase in the number of morulas and blastocysts. |
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