Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres

The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu- PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst r...

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Main Authors:	Ansary, Rezaul Haque, Rahman, Md. Mokhlesur, Awang, Mohamed, Katas, Haliza, Ab. Hadi, Hazrina, Mohamed, Farahidah, Doolaanea, Abd Almonem, Yunus, Kamaruzzaman
Format:	Article
Language:	English English
Published:	Springer 2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/49239/ http://irep.iium.edu.my/49239/ http://irep.iium.edu.my/49239/ http://irep.iium.edu.my/49239/4/49239-article.pdf http://irep.iium.edu.my/49239/10/49239_Preparation%2C%20characterization%20and%20in%20vitro%20release%20study%20of%20BSA_scopus.pdf

id	iium-49239
recordtype	eprints
spelling	iium-492392017-10-16T06:07:49Z http://irep.iium.edu.my/49239/ Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres Ansary, Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Mohamed, Farahidah Doolaanea, Abd Almonem Yunus, Kamaruzzaman QD Chemistry The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu- PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and singlepolymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu- PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins. Springer 2016-01-27 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49239/4/49239-article.pdf application/pdf en http://irep.iium.edu.my/49239/10/49239_Preparation%2C%20characterization%20and%20in%20vitro%20release%20study%20of%20BSA_scopus.pdf Ansary, Rezaul Haque and Rahman, Md. Mokhlesur and Awang, Mohamed and Katas, Haliza and Ab. Hadi, Hazrina and Mohamed, Farahidah and Doolaanea, Abd Almonem and Yunus, Kamaruzzaman (2016) Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres. Archives of Pharmacal Research, 39 (9). pp. 1242-1256. ISSN 0253-6269 E-ISSN 1976-3786 http://link.springer.com/article/10.1007%2Fs12272-016-0710-3 10.1007/s12272-016-0710-3
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Ansary, Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Mohamed, Farahidah Doolaanea, Abd Almonem Yunus, Kamaruzzaman Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres
description	The aim of this study was to prepare a model protein, bovine serum albumin (BSA) loaded double-walled microspheres using a fast degrading glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu- PLGA) and a moderate-degrading carboxyl-terminated PLGA polymers to reduce the initial burst release and to eliminate the lag phase from the release profile of PLGA microspheres. The double-walled microspheres were prepared using a modified water-in-oil-in-oil-in-water (w/o/o/w) method and singlepolymer microspheres were prepared using a conventional water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The particle size, morphology, encapsulation efficiency, thermal properties, in vitro drug release and structural integrity of BSA were evaluated in this study. Double-walled microspheres prepared with Glu-PLGA and PLGA polymers with a mass ratio of 1:1 were non-porous, smooth-surfaced, and spherical in shape. A significant reduction of initial burst release was achieved for the double-walled microspheres compared to single-polymer microspheres. In addition, microspheres prepared using Glu- PLGA and PLGA polymers in a mass ratio of 1:1 exhibited continuous BSA release after the small initial burst without any lag phase. It can be concluded that the double-walled microspheres made of Glu-PLGA and PLGA polymers in a mass ratio of 1:1 can be a potential delivery system for pharmaceutical proteins.
format	Article
author	Ansary, Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Mohamed, Farahidah Doolaanea, Abd Almonem Yunus, Kamaruzzaman
author_facet	Ansary, Rezaul Haque Rahman, Md. Mokhlesur Awang, Mohamed Katas, Haliza Ab. Hadi, Hazrina Mohamed, Farahidah Doolaanea, Abd Almonem Yunus, Kamaruzzaman
author_sort	Ansary, Rezaul Haque
title	Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres
title_short	Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres
title_full	Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres
title_fullStr	Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres
title_full_unstemmed	Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres
title_sort	preparation, characterization and in vitro release study of bsa-loaded double-walled glucose-poly(lactideco- glycolide) microspheres
publisher	Springer
publishDate	2016
url	http://irep.iium.edu.my/49239/ http://irep.iium.edu.my/49239/ http://irep.iium.edu.my/49239/ http://irep.iium.edu.my/49239/4/49239-article.pdf http://irep.iium.edu.my/49239/10/49239_Preparation%2C%20characterization%20and%20in%20vitro%20release%20study%20of%20BSA_scopus.pdf
first_indexed	2023-09-18T21:09:38Z
last_indexed	2023-09-18T21:09:38Z
_version_	1777411165416062976

Preparation, characterization and in vitro release study of BSA-loaded double-walled glucose-poly(lactideco- glycolide) microspheres

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