Effects of water-bath stunning on the death of poultry and myofiber apoptosis

According to the Islamic slaughtering guidelines, swift slaughtering is one of the criteria for good slaughter. In this study, stunning effectiveness in halal poultry meat production was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the ind...

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Main Authors:	Shahdan, Intan Azura, Rahman, Mohammad Tariqur
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	S Agriculture (General)
Online Access:	http://irep.iium.edu.my/49028/ http://irep.iium.edu.my/49028/ http://irep.iium.edu.my/49028/1/49028.pdf

id	iium-49028
recordtype	eprints
spelling	iium-490282018-05-23T06:15:25Z http://irep.iium.edu.my/49028/ Effects of water-bath stunning on the death of poultry and myofiber apoptosis Shahdan, Intan Azura Rahman, Mohammad Tariqur S Agriculture (General) According to the Islamic slaughtering guidelines, swift slaughtering is one of the criteria for good slaughter. In this study, stunning effectiveness in halal poultry meat production was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. Td was recorded using a stopwatch timer, which started at the point of neck cutting and stopped when there were absence of wing flapping, pupil reflex, comb pinching response and heart beat in the chickens. In total, 49 chicken broilers (body weight of 2.17 ± .24 kg) were sacrificed without stunning and with pre-slaughter stunning, using a constant voltage stunner where the current varied between 7.2 to 124.3 mA. The current applied during stunning was found to have no effect on Td. Meat samples from stunned and unstunned chickens were preserved in formalin within 30 min postmortem and apoptotic activity of the muscles were analysed using TUNEL assay and immunohistochemistry. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were not induced in the meat samples of both stunned and unstunned groups at 1 d postmortem. Activations of AIF and caspase 3 lead to DNA fragmentation and apoptosis. Since the slaughtering process and stunning are associated with stress, the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis, by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However, expression of Hsp70 was not induced in both stunned and unstunned groups. This could be due to the short-life span of Hsp70 in vivo, and the very low level of Hsp70 which was undetectable using the method in this study. More details will be shared during the presentation. 2014-12-04 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/49028/1/49028.pdf Shahdan, Intan Azura and Rahman, Mohammad Tariqur (2014) Effects of water-bath stunning on the death of poultry and myofiber apoptosis. In: Malaysia International Halal Research & Education Conference 2014 (MIHREC 2014), 2nd-4th Dec. 2014, Putrajaya. (Unpublished) http://www.mihrec2014.upm.edu.my/program.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	S Agriculture (General)
spellingShingle	S Agriculture (General) Shahdan, Intan Azura Rahman, Mohammad Tariqur Effects of water-bath stunning on the death of poultry and myofiber apoptosis
description	According to the Islamic slaughtering guidelines, swift slaughtering is one of the criteria for good slaughter. In this study, stunning effectiveness in halal poultry meat production was analysed in response to the time needed for the chickens to become insensible upon neck cutting (Td) and the induction of myofiber apoptosis. Td was recorded using a stopwatch timer, which started at the point of neck cutting and stopped when there were absence of wing flapping, pupil reflex, comb pinching response and heart beat in the chickens. In total, 49 chicken broilers (body weight of 2.17 ± .24 kg) were sacrificed without stunning and with pre-slaughter stunning, using a constant voltage stunner where the current varied between 7.2 to 124.3 mA. The current applied during stunning was found to have no effect on Td. Meat samples from stunned and unstunned chickens were preserved in formalin within 30 min postmortem and apoptotic activity of the muscles were analysed using TUNEL assay and immunohistochemistry. Number of apoptotic myonuclei did not vary among stunned and unstunned meat. Apoptosis inducing factor (AIF) and caspase 3 expressions were not induced in the meat samples of both stunned and unstunned groups at 1 d postmortem. Activations of AIF and caspase 3 lead to DNA fragmentation and apoptosis. Since the slaughtering process and stunning are associated with stress, the expression of 70 kDa-heat shock protein (Hsp70) was investigated. Moreover Hsp70 is also an inhibitor of apoptosis, by preventing the activation of AIF and apoptosome which stimulates caspase 3 activation. However, expression of Hsp70 was not induced in both stunned and unstunned groups. This could be due to the short-life span of Hsp70 in vivo, and the very low level of Hsp70 which was undetectable using the method in this study. More details will be shared during the presentation.
format	Conference or Workshop Item
author	Shahdan, Intan Azura Rahman, Mohammad Tariqur
author_facet	Shahdan, Intan Azura Rahman, Mohammad Tariqur
author_sort	Shahdan, Intan Azura
title	Effects of water-bath stunning on the death of poultry and myofiber apoptosis
title_short	Effects of water-bath stunning on the death of poultry and myofiber apoptosis
title_full	Effects of water-bath stunning on the death of poultry and myofiber apoptosis
title_fullStr	Effects of water-bath stunning on the death of poultry and myofiber apoptosis
title_full_unstemmed	Effects of water-bath stunning on the death of poultry and myofiber apoptosis
title_sort	effects of water-bath stunning on the death of poultry and myofiber apoptosis
publishDate	2014
url	http://irep.iium.edu.my/49028/ http://irep.iium.edu.my/49028/ http://irep.iium.edu.my/49028/1/49028.pdf
first_indexed	2023-09-18T21:09:22Z
last_indexed	2023-09-18T21:09:22Z
_version_	1777411149498679296

Effects of water-bath stunning on the death of poultry and myofiber apoptosis

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