Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy

Objectives: To rapidly detect the presence of porcine or bovine gelatins in dental prophylaxis pastes used in Malaysia by using Fourier Transform Infrared Spectroscopy (FTIR). Materials and methods: Four commonly used dental prophylaxis paste brands in Malaysia were collected and labelled A, B...

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Main Authors:	Dewi Ardini, Yunita, Lestari, Widya, Tajudin, Noor Ashikin, Abdul Azis, Nur Fatin
Format:	Conference or Workshop Item
Language:	English English English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/48856/ http://irep.iium.edu.my/48856/1/rapid_detection_halal.pdf http://irep.iium.edu.my/48856/2/cover_4rd_dssc.pdf http://irep.iium.edu.my/48856/3/first_page_4rd_dssc.pdf

id	iium-48856
recordtype	eprints
spelling	iium-488562016-02-05T05:52:01Z http://irep.iium.edu.my/48856/ Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy Dewi Ardini, Yunita Lestari, Widya Tajudin, Noor Ashikin Abdul Azis, Nur Fatin Q Science (General) Objectives: To rapidly detect the presence of porcine or bovine gelatins in dental prophylaxis pastes used in Malaysia by using Fourier Transform Infrared Spectroscopy (FTIR). Materials and methods: Four commonly used dental prophylaxis paste brands in Malaysia were collected and labelled A, B, C, and D. Pure porcine and bovine skin gelatins were used as controls. Gelatin extraction was carried out using acetone precipitation method. The extracts were then placed in the FTIR Perkin Elmer spectrometer for less than 5 minutes. All spectra were recorded within 4000 - 400 cm-I wavenumber with 4 cm-I resolution under 40 scans. The results were analysed by comparing the samples' spectra with the controls'. The data were subjected to discriminant analysis using The Spectrum ™ Software. Results: Sample A and porcine gelatin spectra had similar peak at 2163 cm-I while sample B showed similarity to bovine gelatin at the range of 4000 - 2000 cm-I. Detailed analysis of the spectrum patterns of sample C in the area between 1600-1400 cm-I and 3600-2800 cm-I indicated an appearance of similar peaks to porcine gelatin. On the other hand, the spectrum of sample D at the range of 1800-1200 ern -I was similar to that of bovine gelatin. Conclusion: Bovine and porcine gelatins in dental prophylaxis paste can be rapidly detected using FTIR. Keywords: porcine gelatin, bovine gelatin, FTIR. 2015-03-16 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/48856/1/rapid_detection_halal.pdf application/pdf en http://irep.iium.edu.my/48856/2/cover_4rd_dssc.pdf application/pdf en http://irep.iium.edu.my/48856/3/first_page_4rd_dssc.pdf Dewi Ardini, Yunita and Lestari, Widya and Tajudin, Noor Ashikin and Abdul Azis, Nur Fatin (2015) Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy. In: 4th Dental Student Scientific Conference, 16 March 2015, Kulliyyah of Dentistry IIUM. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Dewi Ardini, Yunita Lestari, Widya Tajudin, Noor Ashikin Abdul Azis, Nur Fatin Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy
description	Objectives: To rapidly detect the presence of porcine or bovine gelatins in dental prophylaxis pastes used in Malaysia by using Fourier Transform Infrared Spectroscopy (FTIR). Materials and methods: Four commonly used dental prophylaxis paste brands in Malaysia were collected and labelled A, B, C, and D. Pure porcine and bovine skin gelatins were used as controls. Gelatin extraction was carried out using acetone precipitation method. The extracts were then placed in the FTIR Perkin Elmer spectrometer for less than 5 minutes. All spectra were recorded within 4000 - 400 cm-I wavenumber with 4 cm-I resolution under 40 scans. The results were analysed by comparing the samples' spectra with the controls'. The data were subjected to discriminant analysis using The Spectrum ™ Software. Results: Sample A and porcine gelatin spectra had similar peak at 2163 cm-I while sample B showed similarity to bovine gelatin at the range of 4000 - 2000 cm-I. Detailed analysis of the spectrum patterns of sample C in the area between 1600-1400 cm-I and 3600-2800 cm-I indicated an appearance of similar peaks to porcine gelatin. On the other hand, the spectrum of sample D at the range of 1800-1200 ern -I was similar to that of bovine gelatin. Conclusion: Bovine and porcine gelatins in dental prophylaxis paste can be rapidly detected using FTIR. Keywords: porcine gelatin, bovine gelatin, FTIR.
format	Conference or Workshop Item
author	Dewi Ardini, Yunita Lestari, Widya Tajudin, Noor Ashikin Abdul Azis, Nur Fatin
author_facet	Dewi Ardini, Yunita Lestari, Widya Tajudin, Noor Ashikin Abdul Azis, Nur Fatin
author_sort	Dewi Ardini, Yunita
title	Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy
title_short	Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy
title_full	Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy
title_fullStr	Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy
title_full_unstemmed	Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy
title_sort	rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy
publishDate	2015
url	http://irep.iium.edu.my/48856/ http://irep.iium.edu.my/48856/1/rapid_detection_halal.pdf http://irep.iium.edu.my/48856/2/cover_4rd_dssc.pdf http://irep.iium.edu.my/48856/3/first_page_4rd_dssc.pdf
first_indexed	2023-09-18T21:09:12Z
last_indexed	2023-09-18T21:09:12Z
_version_	1777411138671083520

Rapid detection of porcine and bovine gelatins in dental prophylaxis pastes using fourier transform infrared spectroscopy

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