Anticancer activity of agarwood distillates on lung cancer cells

Agarwood or gaharu is the fragrant, resinous heartwood that results when some trees, such as Aquilaria malaccensis, are attacked by fungi. Aside from its value as perfume oil, many medicinal claims have been associated with the agarwood itself. This highly-prized heartwood contains a wide array of c...

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Main Authors:	Hussein, Abeir, Hashim, Yumi Zuhanis Has-Yun, Mohd. Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/48820/ http://irep.iium.edu.my/48820/4/Abeir%27s_ACB_2015.pdf http://irep.iium.edu.my/48820/6/48820.docx.pdf

id	iium-48820
recordtype	eprints
spelling	iium-488202016-02-22T17:06:12Z http://irep.iium.edu.my/48820/ Anticancer activity of agarwood distillates on lung cancer cells Hussein, Abeir Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah QD Chemistry Agarwood or gaharu is the fragrant, resinous heartwood that results when some trees, such as Aquilaria malaccensis, are attacked by fungi. Aside from its value as perfume oil, many medicinal claims have been associated with the agarwood itself. This highly-prized heartwood contains a wide array of chemical compounds that fall under various classes of healthy phytochemicals such as terpenoids, alkaloids, and flavonoids. Previous studies have shown promising results when agarwood essential oils were studied for anticancer effects. However, very little information can be found on agarwood distillates, the clear by-products of the agarwood distillation process that possess a characteristic smell, including their therapeutic effects. This paper presents some of the findings in a study of the anticancer activity of agarwood distillates on human lung cancer cells. Two-factor, Face-centred Central Composite Design was used to study the effects of agarwood distillate concentration and time of exposure on attachment (via Trypan blue dye exclusion assay) and cytotoxicity (via MTT assay) of Calu-3 cells cultured in EMEM supplemented with 10% (v/v) FBS. Carried out at three exposure durations (12, 24, and 36 hours), the IC50 values were found to be at concentrations of approximately 20, 80 µL/mL and 150 µL/mL agarwood distillate, respectively, for the Trypan blue dye. The MTT assay yielded IC50 values of approximately 20, 250 µL/mL and 35 µL/mL agarwood distillate, respectively. These results suggest that agarwood distillates possess anti-attachment and cytotoxic effects on Calu-3 lung cancer cells. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/48820/4/Abeir%27s_ACB_2015.pdf application/pdf en http://irep.iium.edu.my/48820/6/48820.docx.pdf Hussein, Abeir and Hashim, Yumi Zuhanis Has-Yun and Mohd. Salleh, Hamzah (2015) Anticancer activity of agarwood distillates on lung cancer cells. In: ASIAN Congress on Biotechnology 2015, 15-19 Nov. 2015, Kuala Lumpur, Malaysia.. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Hussein, Abeir Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Anticancer activity of agarwood distillates on lung cancer cells
description	Agarwood or gaharu is the fragrant, resinous heartwood that results when some trees, such as Aquilaria malaccensis, are attacked by fungi. Aside from its value as perfume oil, many medicinal claims have been associated with the agarwood itself. This highly-prized heartwood contains a wide array of chemical compounds that fall under various classes of healthy phytochemicals such as terpenoids, alkaloids, and flavonoids. Previous studies have shown promising results when agarwood essential oils were studied for anticancer effects. However, very little information can be found on agarwood distillates, the clear by-products of the agarwood distillation process that possess a characteristic smell, including their therapeutic effects. This paper presents some of the findings in a study of the anticancer activity of agarwood distillates on human lung cancer cells. Two-factor, Face-centred Central Composite Design was used to study the effects of agarwood distillate concentration and time of exposure on attachment (via Trypan blue dye exclusion assay) and cytotoxicity (via MTT assay) of Calu-3 cells cultured in EMEM supplemented with 10% (v/v) FBS. Carried out at three exposure durations (12, 24, and 36 hours), the IC50 values were found to be at concentrations of approximately 20, 80 µL/mL and 150 µL/mL agarwood distillate, respectively, for the Trypan blue dye. The MTT assay yielded IC50 values of approximately 20, 250 µL/mL and 35 µL/mL agarwood distillate, respectively. These results suggest that agarwood distillates possess anti-attachment and cytotoxic effects on Calu-3 lung cancer cells.
format	Conference or Workshop Item
author	Hussein, Abeir Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah
author_facet	Hussein, Abeir Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah
author_sort	Hussein, Abeir
title	Anticancer activity of agarwood distillates on lung cancer cells
title_short	Anticancer activity of agarwood distillates on lung cancer cells
title_full	Anticancer activity of agarwood distillates on lung cancer cells
title_fullStr	Anticancer activity of agarwood distillates on lung cancer cells
title_full_unstemmed	Anticancer activity of agarwood distillates on lung cancer cells
title_sort	anticancer activity of agarwood distillates on lung cancer cells
publishDate	2015
url	http://irep.iium.edu.my/48820/ http://irep.iium.edu.my/48820/4/Abeir%27s_ACB_2015.pdf http://irep.iium.edu.my/48820/6/48820.docx.pdf
first_indexed	2023-09-18T21:09:10Z
last_indexed	2023-09-18T21:09:10Z
_version_	1777411136658866176

Anticancer activity of agarwood distillates on lung cancer cells

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