The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection

Widely-used osteomyelitis antibiotic, Gentamicin, was fused with the oil of Black Seed, to form an emulsion and tested for its antimicrobial and antibiofilm properties on an osteomyelitis clinical isolate of S.aureus and a control S.aureus ATCC 29213. The Broth Microdilution Method was used to det...

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Main Authors:	Mohd Shafri, Mohd Affendi, Yaacob, Khairul Ikhwan, Yusof, Fathin Athirah, Shahdan, Intan Azura, Mohd Yusof, Nazri, Mohamed, Farahidah
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/48734/ http://irep.iium.edu.my/48734/ http://irep.iium.edu.my/48734/1/48734.pdf

id	iium-48734
recordtype	eprints
spelling	iium-487342016-02-18T01:33:14Z http://irep.iium.edu.my/48734/ The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection Mohd Shafri, Mohd Affendi Yaacob, Khairul Ikhwan Yusof, Fathin Athirah Shahdan, Intan Azura Mohd Yusof, Nazri Mohamed, Farahidah R Medicine (General) Widely-used osteomyelitis antibiotic, Gentamicin, was fused with the oil of Black Seed, to form an emulsion and tested for its antimicrobial and antibiofilm properties on an osteomyelitis clinical isolate of S.aureus and a control S.aureus ATCC 29213. The Broth Microdilution Method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Briefly, serial dilution of GBSO emulsions was performed on 96 well microtitre plate with each well containing 100 μL bacterial suspension at 108 cfu/ml and incubated for 24 hours at 37°C. The OD reading of each well was taken as the MIC. Content of the well that showed no sign of growth was inoculated on Tryptic Soy Agar (TSA) and incubated overnight for MBC value determination. Next, the effect of the GBSO emulsion on biofilm thickness and viability of bacteria within biofilm was assessed using confocal laser scanning microscope (CLSM). Biofilm of S.aureus, formed on coverslips within 24 hours, were first exposed to GBSO emulsions for 24 hours. After that, all treated biofilm were stained with Live/Dead Baclight stain, viewed with CLSM and images were analyzed using Image J software. In summary, the GBSO emulsion have significantly low MIC and MBC value compared to Gentamicin, and significantly low MBC compared to the Black Seed oil. CLSM analysis showed significant reduction in the thickness of biofilm and highest surface percentage of non-viable (dead) bacteria compared to Gentamicin and N.sativa alone on both strains of S.aureus (clinical isolate and control) after treatment with the GBSO emulsion. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/48734/1/48734.pdf Mohd Shafri, Mohd Affendi and Yaacob, Khairul Ikhwan and Yusof, Fathin Athirah and Shahdan, Intan Azura and Mohd Yusof, Nazri and Mohamed, Farahidah (2015) The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection. In: Eurobiofilms 2015: Fourth European Congress on Microbial Biofilms, 23rd-26th June 2015, Brno, Czech Republic. http://eurobiofilms2015.ics.dk/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mohd Shafri, Mohd Affendi Yaacob, Khairul Ikhwan Yusof, Fathin Athirah Shahdan, Intan Azura Mohd Yusof, Nazri Mohamed, Farahidah The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection
description	Widely-used osteomyelitis antibiotic, Gentamicin, was fused with the oil of Black Seed, to form an emulsion and tested for its antimicrobial and antibiofilm properties on an osteomyelitis clinical isolate of S.aureus and a control S.aureus ATCC 29213. The Broth Microdilution Method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Briefly, serial dilution of GBSO emulsions was performed on 96 well microtitre plate with each well containing 100 μL bacterial suspension at 108 cfu/ml and incubated for 24 hours at 37°C. The OD reading of each well was taken as the MIC. Content of the well that showed no sign of growth was inoculated on Tryptic Soy Agar (TSA) and incubated overnight for MBC value determination. Next, the effect of the GBSO emulsion on biofilm thickness and viability of bacteria within biofilm was assessed using confocal laser scanning microscope (CLSM). Biofilm of S.aureus, formed on coverslips within 24 hours, were first exposed to GBSO emulsions for 24 hours. After that, all treated biofilm were stained with Live/Dead Baclight stain, viewed with CLSM and images were analyzed using Image J software. In summary, the GBSO emulsion have significantly low MIC and MBC value compared to Gentamicin, and significantly low MBC compared to the Black Seed oil. CLSM analysis showed significant reduction in the thickness of biofilm and highest surface percentage of non-viable (dead) bacteria compared to Gentamicin and N.sativa alone on both strains of S.aureus (clinical isolate and control) after treatment with the GBSO emulsion.
format	Conference or Workshop Item
author	Mohd Shafri, Mohd Affendi Yaacob, Khairul Ikhwan Yusof, Fathin Athirah Shahdan, Intan Azura Mohd Yusof, Nazri Mohamed, Farahidah
author_facet	Mohd Shafri, Mohd Affendi Yaacob, Khairul Ikhwan Yusof, Fathin Athirah Shahdan, Intan Azura Mohd Yusof, Nazri Mohamed, Farahidah
author_sort	Mohd Shafri, Mohd Affendi
title	The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection
title_short	The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection
title_full	The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection
title_fullStr	The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection
title_full_unstemmed	The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection
title_sort	potential of novel gentamicin-black seed oil (gbso) fusion emulsion in treating biofilm infection
publishDate	2015
url	http://irep.iium.edu.my/48734/ http://irep.iium.edu.my/48734/ http://irep.iium.edu.my/48734/1/48734.pdf
first_indexed	2023-09-18T21:09:04Z
last_indexed	2023-09-18T21:09:04Z
_version_	1777411130589708288

The potential of novel gentamicin-black seed oil (GBSO) fusion emulsion in treating biofilm infection

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