Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan

Aims: Accurate diagnosis and proper treatments of osteomyelitis are often difficult and ineffective due to several reasons such as less sensitive sample collected and the formation of biofilm following prosthetic use. Thus, our goal of this study is to identify suitable sample for laboratory diagn...

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Main Authors:	Yaacob, Khairul Ikhwan, Mohd Yusof, Nazri, Mohamed, Farahidah, Mohd Shafri, Mohd Affendi
Format:	Article
Language:	English
Published:	Malaysian Society for Microbiology 2015
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/48689/ http://irep.iium.edu.my/48689/ http://irep.iium.edu.my/48689/1/1-Vol_11-4-MJM692-14_%28FINAL%29.pdf

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spelling	iium-486892016-07-17T08:57:40Z http://irep.iium.edu.my/48689/ Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi R Medicine (General) Aims: Accurate diagnosis and proper treatments of osteomyelitis are often difficult and ineffective due to several reasons such as less sensitive sample collected and the formation of biofilm following prosthetic use. Thus, our goal of this study is to identify suitable sample for laboratory diagnosis and also microbial species that cause osteomyelitic infection and discriminate between biofilm and non-biofilm producing strains in patients at Hospital Tengku Ampuan Afzan, Kuantan. Methodology and results: Samples of bone, prosthetic material, tissue and swab were collected from patient with suspected osteomyelitis at the hospital. Bacteria were isolated from sample using methods such as homogenization, direct transfer, and sonication. Then, species identification was done by colony characterization, biochemical test and the API identification system. Once species identified, tissue culture plate method was performed to discriminate the biofilm-producing strain from the non-biofilm-producing strain. The total number of 57 samples were collected from 17 cases of suspected osteomyelitis with 34 samples were found positive bacterial growth. Prosthetic samples produced highest positive growth with 81.3%, following by bone samples with 66.7% while swab and tissue samples with 46.2% and 43.8% respectively. We found that 14 from total 16 pathogens identified were biofilm producing-strains. Conclusion, significance, and impact of study: Prosthetic and bone samples produced higher bacterial growth, in contrast to other type of samples. Sonication method improves bacterial detection. Biofilm producing-bacteria were also the most common isolated strains from osteomyelitic infection. These have underscored the need to revise current clinical and laboratory practice as proper identification biofilm bacteria may influences management an outcome. Malaysian Society for Microbiology 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/48689/1/1-Vol_11-4-MJM692-14_%28FINAL%29.pdf Yaacob, Khairul Ikhwan and Mohd Yusof, Nazri and Mohamed, Farahidah and Mohd Shafri, Mohd Affendi (2015) Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan. Malaysian Journal of Microbiology, 11 (4). pp. 317-323. ISSN 1823-8262 E-ISSN 2231-7538 http://mjm.usm.my/uploads/issues/526/1-Vol%2011-4-MJM692-14%20(FINAL).pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan
description	Aims: Accurate diagnosis and proper treatments of osteomyelitis are often difficult and ineffective due to several reasons such as less sensitive sample collected and the formation of biofilm following prosthetic use. Thus, our goal of this study is to identify suitable sample for laboratory diagnosis and also microbial species that cause osteomyelitic infection and discriminate between biofilm and non-biofilm producing strains in patients at Hospital Tengku Ampuan Afzan, Kuantan. Methodology and results: Samples of bone, prosthetic material, tissue and swab were collected from patient with suspected osteomyelitis at the hospital. Bacteria were isolated from sample using methods such as homogenization, direct transfer, and sonication. Then, species identification was done by colony characterization, biochemical test and the API identification system. Once species identified, tissue culture plate method was performed to discriminate the biofilm-producing strain from the non-biofilm-producing strain. The total number of 57 samples were collected from 17 cases of suspected osteomyelitis with 34 samples were found positive bacterial growth. Prosthetic samples produced highest positive growth with 81.3%, following by bone samples with 66.7% while swab and tissue samples with 46.2% and 43.8% respectively. We found that 14 from total 16 pathogens identified were biofilm producing-strains. Conclusion, significance, and impact of study: Prosthetic and bone samples produced higher bacterial growth, in contrast to other type of samples. Sonication method improves bacterial detection. Biofilm producing-bacteria were also the most common isolated strains from osteomyelitic infection. These have underscored the need to revise current clinical and laboratory practice as proper identification biofilm bacteria may influences management an outcome.
format	Article
author	Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi
author_facet	Yaacob, Khairul Ikhwan Mohd Yusof, Nazri Mohamed, Farahidah Mohd Shafri, Mohd Affendi
author_sort	Yaacob, Khairul Ikhwan
title	Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan
title_short	Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan
title_full	Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan
title_fullStr	Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan
title_full_unstemmed	Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan
title_sort	isolation of the biofilm producing bacteria from osteomyelitis patients at hospital tengku ampuan afzan (htaa), kuantan
publisher	Malaysian Society for Microbiology
publishDate	2015
url	http://irep.iium.edu.my/48689/ http://irep.iium.edu.my/48689/ http://irep.iium.edu.my/48689/1/1-Vol_11-4-MJM692-14_%28FINAL%29.pdf
first_indexed	2023-09-18T21:09:01Z
last_indexed	2023-09-18T21:09:01Z
_version_	1777411126683762688

Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan

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