Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)

Cross-linked enzyme aggregates (CLEA) technology has been gaining more attention recently due to their advantages in industrial application. Various CLEA- enzymes has been successfully prepared with diverse advantages. Among the benefits of CLEA are; more stability, easy to prepare, cheap and reus...

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Main Authors:	Easa, Muhammad Noor, Yusof, Faridah
Format:	Article
Language:	English
Published:	Asian Research Publishing Network (ARPN) 2015
Subjects:	Q Science (General) T Technology (General) TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/48227/ http://irep.iium.edu.my/48227/ http://irep.iium.edu.my/48227/1/P_72.pdf

id	iium-48227
recordtype	eprints
spelling	iium-482272017-10-31T00:50:31Z http://irep.iium.edu.my/48227/ Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio) Easa, Muhammad Noor Yusof, Faridah Q Science (General) T Technology (General) TP248.13 Biotechnology Cross-linked enzyme aggregates (CLEA) technology has been gaining more attention recently due to their advantages in industrial application. Various CLEA- enzymes has been successfully prepared with diverse advantages. Among the benefits of CLEA are; more stability, easy to prepare, cheap and reusable as compared to the free enzymes. This particular study has attempted to optimize the production of CLEA-amylase, which sourced from supermeal worm protein extract. One Factor at a Time (OFAT) and Response Surface Methodology (RSM) had been used to achieve the goal. Screening of several additives had been conducted to enhance the recovery activity of CLEA- amylase. The final preparation of CLEAamylase was done by applying acetone as precipitant, glutaraldehyde as cross-linking agent and BSA as proteic feeder. Under Face Centered Central Composite Design (FCCCD), the interactions between those three influential parameters were observed. From the 20 runs, the optimum activity was recorded at Run 11 (26.65%), under the condition of using 60% acetone, 90mM glutaraldehyde and 2.5 mg/ml bovine serume albumin (BSA) as additive. The model equation was considered valid after been tested through validation test. Acetone and glutaraldehyde concentration had shown the most dominant factor from this study, while BSA concentration had insignificant effects. Asian Research Publishing Network (ARPN) 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/48227/1/P_72.pdf Easa, Muhammad Noor and Yusof, Faridah (2015) Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio). ARPN Journal of Engineering and Applied Sciences, 10 (21). pp. 9710-9716. ISSN 1819-6608 http://www.arpnjournals.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) T Technology (General) TP248.13 Biotechnology
spellingShingle	Q Science (General) T Technology (General) TP248.13 Biotechnology Easa, Muhammad Noor Yusof, Faridah Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)
description	Cross-linked enzyme aggregates (CLEA) technology has been gaining more attention recently due to their advantages in industrial application. Various CLEA- enzymes has been successfully prepared with diverse advantages. Among the benefits of CLEA are; more stability, easy to prepare, cheap and reusable as compared to the free enzymes. This particular study has attempted to optimize the production of CLEA-amylase, which sourced from supermeal worm protein extract. One Factor at a Time (OFAT) and Response Surface Methodology (RSM) had been used to achieve the goal. Screening of several additives had been conducted to enhance the recovery activity of CLEA- amylase. The final preparation of CLEAamylase was done by applying acetone as precipitant, glutaraldehyde as cross-linking agent and BSA as proteic feeder. Under Face Centered Central Composite Design (FCCCD), the interactions between those three influential parameters were observed. From the 20 runs, the optimum activity was recorded at Run 11 (26.65%), under the condition of using 60% acetone, 90mM glutaraldehyde and 2.5 mg/ml bovine serume albumin (BSA) as additive. The model equation was considered valid after been tested through validation test. Acetone and glutaraldehyde concentration had shown the most dominant factor from this study, while BSA concentration had insignificant effects.
format	Article
author	Easa, Muhammad Noor Yusof, Faridah
author_facet	Easa, Muhammad Noor Yusof, Faridah
author_sort	Easa, Muhammad Noor
title	Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)
title_short	Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)
title_full	Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)
title_fullStr	Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)
title_full_unstemmed	Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)
title_sort	optimizing the preparation of cross-linked enzyme aggregates (clea)- amylase from supermeal worm (zophobas morio)
publisher	Asian Research Publishing Network (ARPN)
publishDate	2015
url	http://irep.iium.edu.my/48227/ http://irep.iium.edu.my/48227/ http://irep.iium.edu.my/48227/1/P_72.pdf
first_indexed	2023-09-18T21:08:27Z
last_indexed	2023-09-18T21:08:27Z
_version_	1777411091528155136

Optimizing the preparation of Cross-Linked Enzyme Aggregates (CLEA)- amylase from supermeal worm (Zophobas morio)

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