Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication

Microfibrillated cellulose (MFC) can be used as rheology modifiers in foods, paints, cosmetics and pharmaceutical products as well as fillers in composite materials. It can be isolated from different lignocellulosic materials using different technologies. In this study, MFC was produced from the sub...

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Main Authors:	Jimat, Dzun Noraini, Avicenna, Avicenna
Format:	Conference or Workshop Item
Language:	English English English
Published:	2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/48061/ http://irep.iium.edu.my/48061/ http://irep.iium.edu.my/48061/2/ACB_Slide_Avicenna.pdf http://irep.iium.edu.my/48061/3/BTJ-Avicenna-IIUM_2_paper.pdf http://irep.iium.edu.my/48061/9/48061.pdf

id	iium-48061
recordtype	eprints
spelling	iium-480612016-02-03T03:23:44Z http://irep.iium.edu.my/48061/ Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication Jimat, Dzun Noraini Avicenna, Avicenna TP248.13 Biotechnology Microfibrillated cellulose (MFC) can be used as rheology modifiers in foods, paints, cosmetics and pharmaceutical products as well as fillers in composite materials. It can be isolated from different lignocellulosic materials using different technologies. In this study, MFC was produced from the substrate of cocoa pod husk (CPH) in two distinct processes. Substrate was subjected to fungal-pretreatment at the first stage through a solid state fermentation by Aspergillus niger to promote enzymatic splitting of cellulose. The obtained fungal pretreated substrate was then mechanically processed by the application of high-intensity ultrasonication. The objective of the research was to define the potential and the optimum process of the fungal pretreatment stage through a response surface design (RSM) with substrate raw particle size (RPS) and inoculums size (IS) as independent variables. Statistical result of RSM showed that the model follows a quadratic response with IS as significant term. The 3D model graph exhibit an inverted peak response surface with maximum particle size of MFC at 2,761 nm at RPH of 850 micron and 5.0 % IS. Results from SEM images showed that the structure and morphology of the resulted MFC differed over the various level of IS with approximated diameter of MFC at 30 – 50 micron. Fungal-pretreatment also proven to remove amorphous structure of hemicelluloses and lignin giving a loosen structure that appeared to be at the size of about 50 µm to certain hundreds micrometer. FTIR results indicated a prominent change of spectra over the stages with a notable peak at the range of 3600 cm-1 and 2600 cm-1 appeared as a vibration of the OH-stretching and CH-stretching. The approximated peak of 1730 cm-1 from the raw substrate indicates the presence of hemicellulose, where at the later samples of the fungal pretreated substrate, the spectra showed a lower intensity, and mostly disappeared after the process of ultrasonication. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/48061/2/ACB_Slide_Avicenna.pdf application/pdf en http://irep.iium.edu.my/48061/3/BTJ-Avicenna-IIUM_2_paper.pdf application/pdf en http://irep.iium.edu.my/48061/9/48061.pdf Jimat, Dzun Noraini and Avicenna, Avicenna (2015) Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication. In: Asian Congress on Biotechnology 2015 (ACB 2015), 15th-19th Nov 2015, Istana Hotel, Kuala Lumpur. (Unpublished) http://www.acb2015.my/web/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Jimat, Dzun Noraini Avicenna, Avicenna Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication
description	Microfibrillated cellulose (MFC) can be used as rheology modifiers in foods, paints, cosmetics and pharmaceutical products as well as fillers in composite materials. It can be isolated from different lignocellulosic materials using different technologies. In this study, MFC was produced from the substrate of cocoa pod husk (CPH) in two distinct processes. Substrate was subjected to fungal-pretreatment at the first stage through a solid state fermentation by Aspergillus niger to promote enzymatic splitting of cellulose. The obtained fungal pretreated substrate was then mechanically processed by the application of high-intensity ultrasonication. The objective of the research was to define the potential and the optimum process of the fungal pretreatment stage through a response surface design (RSM) with substrate raw particle size (RPS) and inoculums size (IS) as independent variables. Statistical result of RSM showed that the model follows a quadratic response with IS as significant term. The 3D model graph exhibit an inverted peak response surface with maximum particle size of MFC at 2,761 nm at RPH of 850 micron and 5.0 % IS. Results from SEM images showed that the structure and morphology of the resulted MFC differed over the various level of IS with approximated diameter of MFC at 30 – 50 micron. Fungal-pretreatment also proven to remove amorphous structure of hemicelluloses and lignin giving a loosen structure that appeared to be at the size of about 50 µm to certain hundreds micrometer. FTIR results indicated a prominent change of spectra over the stages with a notable peak at the range of 3600 cm-1 and 2600 cm-1 appeared as a vibration of the OH-stretching and CH-stretching. The approximated peak of 1730 cm-1 from the raw substrate indicates the presence of hemicellulose, where at the later samples of the fungal pretreated substrate, the spectra showed a lower intensity, and mostly disappeared after the process of ultrasonication.
format	Conference or Workshop Item
author	Jimat, Dzun Noraini Avicenna, Avicenna
author_facet	Jimat, Dzun Noraini Avicenna, Avicenna
author_sort	Jimat, Dzun Noraini
title	Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication
title_short	Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication
title_full	Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication
title_fullStr	Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication
title_full_unstemmed	Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication
title_sort	process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication
publishDate	2015
url	http://irep.iium.edu.my/48061/ http://irep.iium.edu.my/48061/ http://irep.iium.edu.my/48061/2/ACB_Slide_Avicenna.pdf http://irep.iium.edu.my/48061/3/BTJ-Avicenna-IIUM_2_paper.pdf http://irep.iium.edu.my/48061/9/48061.pdf
first_indexed	2023-09-18T21:08:17Z
last_indexed	2023-09-18T21:08:17Z
_version_	1777411080941731840

Process improvement on the production of microfibrillated cellulose using fungal pretreatment and ultrasonication

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