Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis

Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis

Palm kernel cake (PKC) was utilized as the main media for cellulase enzyme production using solid-state bioconversion (SSB). The fermentation was carried out for 7 days using Trichoderma reesei with 0.05% Tween-80 and moisture content of 62.0% (w/w). The enzyme was extracted with citrate buffer (pH...

Full description

Bibliographic Details
Main Authors: Elgharbawy, Amal Ahmed, Alam, Md. Zahangir, Jamal, Parveen, Moniruzzaman, Md.
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
TP155 Chemical engineering
Online Access:http://irep.iium.edu.my/47895/
http://irep.iium.edu.my/47895/
http://irep.iium.edu.my/47895/1/47895.pdf
id iium-47895
recordtype eprints
spelling iium-478952016-02-02T03:13:22Z http://irep.iium.edu.my/47895/ Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis Elgharbawy, Amal Ahmed Alam, Md. Zahangir Jamal, Parveen Moniruzzaman, Md. TP155 Chemical engineering Palm kernel cake (PKC) was utilized as the main media for cellulase enzyme production using solid-state bioconversion (SSB). The fermentation was carried out for 7 days using Trichoderma reesei with 0.05% Tween-80 and moisture content of 62.0% (w/w). The enzyme was extracted with citrate buffer (pH 4.8) at 150 rpm agitation speed. The enzyme extract was centrifuged and then purified by ultrafiltration membrane. The activity of the cellulase was determined by carboxymethyl cellulose (CMC) assay at 72.45 Unit/ml (603.75 U/ g of dry PKC). The PKC-cellulase (PKC-Cel) was tested in several ionic liquids (ILs) for its compatibility and stability as a combined system and the residual activity was calculated by taking the IL-free enzyme as the control (100%). The ionic liquids were: choline acetate (CoAc), 1-ethyl-3-methylimidazolium acetate (EMIMAc), dimethylimidazolium dimethylphosphate 90.45±0.44% of its activity in the concentration of 10.0, 20.0 and 40.0% of the (IL/citrate buffer) solution, where at 60.0 and 80.0%, 76.66±2.46 activity was retained. At a concentration of 100%, 63.16±0.04 of the initial activity was maintained for a duration of 6 hours. On the other hand, at 100% of EMIMAc, PKC-Cel could only sustain 4.75±0.2315% of the initial activity. In contrast, 95.62±0.68% was maintained in EM- DEP in concentration varied at 10, 20 and 40% of the (IL/buffer) whereas 36.03±0.46% remained in the pure IL. In DM-DMP, PKC-Cel was stable with no loss of its activity at 10.0, 20.0 and 40% for the first 3 hours, retaining only 8.17±0.28% of its activity after 6 hours. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47895/1/47895.pdf Elgharbawy, Amal Ahmed and Alam, Md. Zahangir and Jamal, Parveen and Moniruzzaman, Md. (2015) Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis. In: Asian Congress on Biotechnology 2015 (ACB 2015), 15th-19th Nov 2015, Istana Hotel, Kuala Lumpur. (Unpublished) http://www.acb2015.my/web/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP155 Chemical engineering
spellingShingle TP155 Chemical engineering
Elgharbawy, Amal Ahmed
Alam, Md. Zahangir
Jamal, Parveen
Moniruzzaman, Md.
Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis
description Palm kernel cake (PKC) was utilized as the main media for cellulase enzyme production using solid-state bioconversion (SSB). The fermentation was carried out for 7 days using Trichoderma reesei with 0.05% Tween-80 and moisture content of 62.0% (w/w). The enzyme was extracted with citrate buffer (pH 4.8) at 150 rpm agitation speed. The enzyme extract was centrifuged and then purified by ultrafiltration membrane. The activity of the cellulase was determined by carboxymethyl cellulose (CMC) assay at 72.45 Unit/ml (603.75 U/ g of dry PKC). The PKC-cellulase (PKC-Cel) was tested in several ionic liquids (ILs) for its compatibility and stability as a combined system and the residual activity was calculated by taking the IL-free enzyme as the control (100%). The ionic liquids were: choline acetate (CoAc), 1-ethyl-3-methylimidazolium acetate (EMIMAc), dimethylimidazolium dimethylphosphate 90.45±0.44% of its activity in the concentration of 10.0, 20.0 and 40.0% of the (IL/citrate buffer) solution, where at 60.0 and 80.0%, 76.66±2.46 activity was retained. At a concentration of 100%, 63.16±0.04 of the initial activity was maintained for a duration of 6 hours. On the other hand, at 100% of EMIMAc, PKC-Cel could only sustain 4.75±0.2315% of the initial activity. In contrast, 95.62±0.68% was maintained in EM- DEP in concentration varied at 10, 20 and 40% of the (IL/buffer) whereas 36.03±0.46% remained in the pure IL. In DM-DMP, PKC-Cel was stable with no loss of its activity at 10.0, 20.0 and 40% for the first 3 hours, retaining only 8.17±0.28% of its activity after 6 hours.
format Conference or Workshop Item
author Elgharbawy, Amal Ahmed
Alam, Md. Zahangir
Jamal, Parveen
Moniruzzaman, Md.
author_facet Elgharbawy, Amal Ahmed
Alam, Md. Zahangir
Jamal, Parveen
Moniruzzaman, Md.
author_sort Elgharbawy, Amal Ahmed
title Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis
title_short Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis
title_full Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis
title_fullStr Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis
title_full_unstemmed Ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis
title_sort ionic liquid-tolerant cellulase from palm kernel cake for efficient lignocellulose hydrolysis
publishDate 2015
url http://irep.iium.edu.my/47895/
http://irep.iium.edu.my/47895/
http://irep.iium.edu.my/47895/1/47895.pdf
first_indexed 2023-09-18T21:08:05Z
last_indexed 2023-09-18T21:08:05Z
_version_ 1777411068269690880

Similar Items