Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea

This study aimed to identify the properties as well as the application of Candida cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solve...

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Main Authors:	Elgharbawy, Amal A.M., Alam, Md. Zahangir, Mohd. Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English English
Published:	2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/47894/ http://irep.iium.edu.my/47894/ http://irep.iium.edu.my/47894/1/CHARACTERIZATION_OF_LOW-ID28.pdf http://irep.iium.edu.my/47894/4/47894.pdf

id	iium-47894
recordtype	eprints
spelling	iium-478942019-04-17T02:08:53Z http://irep.iium.edu.my/47894/ Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea Elgharbawy, Amal A.M. Alam, Md. Zahangir Mohd. Salleh, Hamzah TP248.13 Biotechnology This study aimed to identify the properties as well as the application of Candida cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solvents and metal ions solutions. The enzyme assays were conducted using colorimetric method and the activity was expressed as residual activity. It was revealed that the optimal temperature and pH were 37°C and 8.0, respectively. The enzyme was stable at temperature ranges of 25-45°C and pH 7.0-8.0 respectively. Zn2+, Mg2+ and Ca2+ stimulated the enzyme while Fe3+ inhibited its activity. The results showed that 1.0% of EDTA enhanced the lipase activity while incubation in SDS and Tween-80 resulted in the reduction of its activity. Moreover, the produced lipase exhibited some levels of stability at low concentrations of organic solvents (methanol, ethanol, 2-propanol, acetone, hexane and toluene). The application of the PKC-lipase showed promising results in hydrolysis whereas 50.48% and 45.98% of canola oil and olive oil were converted to free fatty acids (FFA), respectively. The knowledge obtained from this study can give an insight on understanding the PKC-lipase properties in order to be used in industrial and biotechnological applications, particularly in biodiesel production. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47894/1/CHARACTERIZATION_OF_LOW-ID28.pdf application/pdf en http://irep.iium.edu.my/47894/4/47894.pdf Elgharbawy, Amal A.M. and Alam, Md. Zahangir and Mohd. Salleh, Hamzah (2015) Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea. In: International Postgraduate Conference on Engineering Research (IPCER) 2015 , 27th-28th Oct. 2015, Gombak Campus, IIUM. (Unpublished) http://www.iium.edu.my/ipcer/15/index.php/paper-format/2-uncategorised
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Elgharbawy, Amal A.M. Alam, Md. Zahangir Mohd. Salleh, Hamzah Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea
description	This study aimed to identify the properties as well as the application of Candida cylindracea lipase produced from palm kernel cake (PKC) by solid-state fermentation (SSF). Characteristics of PKC-lipase have been investigated which include: thermal stability, pH stability, stability in organic solvents and metal ions solutions. The enzyme assays were conducted using colorimetric method and the activity was expressed as residual activity. It was revealed that the optimal temperature and pH were 37°C and 8.0, respectively. The enzyme was stable at temperature ranges of 25-45°C and pH 7.0-8.0 respectively. Zn2+, Mg2+ and Ca2+ stimulated the enzyme while Fe3+ inhibited its activity. The results showed that 1.0% of EDTA enhanced the lipase activity while incubation in SDS and Tween-80 resulted in the reduction of its activity. Moreover, the produced lipase exhibited some levels of stability at low concentrations of organic solvents (methanol, ethanol, 2-propanol, acetone, hexane and toluene). The application of the PKC-lipase showed promising results in hydrolysis whereas 50.48% and 45.98% of canola oil and olive oil were converted to free fatty acids (FFA), respectively. The knowledge obtained from this study can give an insight on understanding the PKC-lipase properties in order to be used in industrial and biotechnological applications, particularly in biodiesel production.
format	Conference or Workshop Item
author	Elgharbawy, Amal A.M. Alam, Md. Zahangir Mohd. Salleh, Hamzah
author_facet	Elgharbawy, Amal A.M. Alam, Md. Zahangir Mohd. Salleh, Hamzah
author_sort	Elgharbawy, Amal A.M.
title	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea
title_short	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea
title_full	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea
title_fullStr	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea
title_full_unstemmed	Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea
title_sort	characterization of low-cost lipase by solid-state fermentation of palm kernel cake using candida cylindracea
publishDate	2015
url	http://irep.iium.edu.my/47894/ http://irep.iium.edu.my/47894/ http://irep.iium.edu.my/47894/1/CHARACTERIZATION_OF_LOW-ID28.pdf http://irep.iium.edu.my/47894/4/47894.pdf
first_indexed	2023-09-18T21:08:05Z
last_indexed	2023-09-18T21:08:05Z
_version_	1777411068129181696

Characterization of low-cost lipase by solid-state fermentation of palm kernel cake using Candida Cylindracea

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