Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat

Combination therapy is an effective strategy in management of many health problems. There is some evidence which support a pharmaco-mechanistic way to antiepileptic drugs (AEDs) combination. The effectiveness of combination therapy as a treatment strategy for epilepsy and diabetic neuropathy is unde...

Full description

Bibliographic Details
Main Authors:	Abdullah, Sinan Mohammed, Abdul Razak, Tariq, Al-Ani, Imad Matloub Dally, Abdualkader, Abdualrahman Mohammed
Format:	Conference or Workshop Item
Language:	English English
Published:	2014
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/47666/ http://irep.iium.edu.my/47666/ http://irep.iium.edu.my/47666/1/IRIIE_2014_sinan_339.pdf http://irep.iium.edu.my/47666/4/47666.pdf

id	iium-47666
recordtype	eprints
spelling	iium-476662016-03-23T07:18:49Z http://irep.iium.edu.my/47666/ Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally Abdualkader, Abdualrahman Mohammed RM Therapeutics. Pharmacology Combination therapy is an effective strategy in management of many health problems. There is some evidence which support a pharmaco-mechanistic way to antiepileptic drugs (AEDs) combination. The effectiveness of combination therapy as a treatment strategy for epilepsy and diabetic neuropathy is undergoing reassessment. This is a result of the increasing gratitude that all seizures and neuropathy pain cannot be managed by monotherapy in a significant percentage of patients, and of the development of a range of new AEDs some of these medications are well tolerated and less prone to complex pharmacokinetic drug interactions than their older generations. A combination of two antiepileptic drugs can be used when there is absence of benefit of one or two different monotherapy regimens. The main objective of this research is to investigate the unwanted effects of using high therapeutic doses in case of combination between two medications. Thirty five adult female Sprague–Dawley rats weighing 200–225 g are used in this experiment. All animals were housed in groups of 2-3 in a temperature (22±1 ºc), relative-humidity (60–70%) controlled room on a 12:12-h light/dark cycle and allowed free access to water and normal diet for rodents. Animal care complied with that stipulated by the local ethics committee. The rats were randomly divided into 5 groups of 7 animals each as follows: group 1= GBP (250mg/kg), group 2= CBZ (80 mg/kg), group 3=GBP+CBZ (250+80 mg/kg), group 4=GBP+CBZ (125+40 mg/kg), group 5= control (distilled water) (0.6 ml/day). Gabapentin (Neurontin, Pfizer, USA) was suspended in distilled water and administered 3 times daily. Carbamazepine liquid (Novartis, Switzerland) administered one times daily. Drugs were administered to rats by oral gavage method for one month. Animals from control and treated groups were sacrificed; dissected and small pieces of the liver and kidney were quickly removed, then fixed in 10% formalin. Following fixation, specimens were dehydrated, embedded, and then sectioned to 4 microns thickness. For histological examinations, sections were stained with Ehrlich Haematoxylin and Eosin. Blood samples are collected from all groups for biochemicals analysis. In conclusion, treatment with maximum therapeutic dose of GBP, CBZ and their combination may cause serious adverse effects. In spite of there is no pharmacokinetic interaction between these medications but there are many histopathological and biochemical abnormalities found in this study. Finally, combination therapies need to examine thoroughly regarding efficacy and unwanted effects to avoid any serious complications. 2014 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/47666/1/IRIIE_2014_sinan_339.pdf application/pdf en http://irep.iium.edu.my/47666/4/47666.pdf Abdullah, Sinan Mohammed and Abdul Razak, Tariq and Al-Ani, Imad Matloub Dally and Abdualkader, Abdualrahman Mohammed (2014) Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat. In: International Research, Invention and Innovation Exhibition 2014 (IRIIE2014), 11th -13th June 2014, Cultural Activity Center (CAC), Internatioanal Islamic University Malaysia. (Unpublished) http://www.iium.edu.my/irie/14/index.php/result/8-irie2014/36-results-for-has
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally Abdualkader, Abdualrahman Mohammed Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat
description	Combination therapy is an effective strategy in management of many health problems. There is some evidence which support a pharmaco-mechanistic way to antiepileptic drugs (AEDs) combination. The effectiveness of combination therapy as a treatment strategy for epilepsy and diabetic neuropathy is undergoing reassessment. This is a result of the increasing gratitude that all seizures and neuropathy pain cannot be managed by monotherapy in a significant percentage of patients, and of the development of a range of new AEDs some of these medications are well tolerated and less prone to complex pharmacokinetic drug interactions than their older generations. A combination of two antiepileptic drugs can be used when there is absence of benefit of one or two different monotherapy regimens. The main objective of this research is to investigate the unwanted effects of using high therapeutic doses in case of combination between two medications. Thirty five adult female Sprague–Dawley rats weighing 200–225 g are used in this experiment. All animals were housed in groups of 2-3 in a temperature (22±1 ºc), relative-humidity (60–70%) controlled room on a 12:12-h light/dark cycle and allowed free access to water and normal diet for rodents. Animal care complied with that stipulated by the local ethics committee. The rats were randomly divided into 5 groups of 7 animals each as follows: group 1= GBP (250mg/kg), group 2= CBZ (80 mg/kg), group 3=GBP+CBZ (250+80 mg/kg), group 4=GBP+CBZ (125+40 mg/kg), group 5= control (distilled water) (0.6 ml/day). Gabapentin (Neurontin, Pfizer, USA) was suspended in distilled water and administered 3 times daily. Carbamazepine liquid (Novartis, Switzerland) administered one times daily. Drugs were administered to rats by oral gavage method for one month. Animals from control and treated groups were sacrificed; dissected and small pieces of the liver and kidney were quickly removed, then fixed in 10% formalin. Following fixation, specimens were dehydrated, embedded, and then sectioned to 4 microns thickness. For histological examinations, sections were stained with Ehrlich Haematoxylin and Eosin. Blood samples are collected from all groups for biochemicals analysis. In conclusion, treatment with maximum therapeutic dose of GBP, CBZ and their combination may cause serious adverse effects. In spite of there is no pharmacokinetic interaction between these medications but there are many histopathological and biochemical abnormalities found in this study. Finally, combination therapies need to examine thoroughly regarding efficacy and unwanted effects to avoid any serious complications.
format	Conference or Workshop Item
author	Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally Abdualkader, Abdualrahman Mohammed
author_facet	Abdullah, Sinan Mohammed Abdul Razak, Tariq Al-Ani, Imad Matloub Dally Abdualkader, Abdualrahman Mohammed
author_sort	Abdullah, Sinan Mohammed
title	Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat
title_short	Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat
title_full	Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat
title_fullStr	Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat
title_full_unstemmed	Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat
title_sort	toxicological profiles of carbamazepine, gabapentin and their combination at high therapeutic doses in rat
publishDate	2014
url	http://irep.iium.edu.my/47666/ http://irep.iium.edu.my/47666/ http://irep.iium.edu.my/47666/1/IRIIE_2014_sinan_339.pdf http://irep.iium.edu.my/47666/4/47666.pdf
first_indexed	2023-09-18T21:07:48Z
last_indexed	2023-09-18T21:07:48Z
_version_	1777411050602233856

Toxicological profiles of Carbamazepine, Gabapentin and their combination at high therapeutic doses in rat

Similar Items