Protein enrichment of fruit peels as animal feed supplement by mixed phanerochaete chrysosporium and Schizophyllum commune
Optimization of basidiomycete mixed cultures for production of animal feed supplement in a solid state fermentation (SSF) remained elusive. Process conditions associated with improved protein enrichment of fruit peels by two white rot fungi were optimized using response surface methodology (RSM)....
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47565/ http://irep.iium.edu.my/47565/ http://irep.iium.edu.my/47565/1/acb_abs_1s.pdf |
| Summary: | Optimization of basidiomycete mixed cultures for production of animal feed
supplement in a solid state fermentation (SSF) remained elusive. Process conditions
associated with improved protein enrichment of fruit peels by two white rot fungi were
optimized using response surface methodology (RSM). Through one-factor-at-a-time
(OFAT) method, six day fermentation period and temperature of 32°C were optimum
for elevated protein synthesis and enzyme activities. Face-Centered Central Composite
Design (FCCCD) was used to determine levels and effects of process parameters: pH,
inoculum size and moisture content on the protein enrichment of fruit peels. A highly
significant quadratic model obtained from FCCCD described optimization path. Results
of the statistical analysis showed a significant (P < 0.05) linear effect for pH and
inoculum size; while pH and moisture content significantly (P < 0.01) interact. The
optimum process combination was found to be 70.2% (v/w) of moisture content, a pH
of 5.4 and 6.1% (v/w) inoculum size. A maximum protein content of 198.77 mg/g was
obtained during 6-day of solid-state fermentation. Amino acid concentration of the
optimized product suited supplement for broiler feed. |
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