Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration

Devoid of scientific proofs, the use of commercially available natural product-based eye toners for therapeutic purposes incites controversies surrounding it. This study aims to evaluate the effect of selected commercially available eye toners on in vitro cellular model using rabbits’ corneal kerato...

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Main Authors:	Mohamad Sukri, Norhamiza, Razali, Rabiatul Adawiyah, Arifin, Nurfadzillah, Harun, Nurul Athirah, Mohd Azid @ Nazid, Ruwaida, Suboh, Ruzira, Mohamad Sofian, Nurul Damia, Abdul Rahman, Suzanah, Abdul Rahim, Muhd Afzam Shah, Sha'ban, Munirah
Format:	Article
Language:	English
Published:	Tissue Engineering and Regenerative Medicine Society of Malaysia 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/47518/ http://irep.iium.edu.my/47518/ http://irep.iium.edu.my/47518/1/15_REGENERATIVE_RESEARCH_2015.pdf

id	iium-47518
recordtype	eprints
spelling	iium-475182016-07-15T02:53:32Z http://irep.iium.edu.my/47518/ Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration Mohamad Sukri, Norhamiza Razali, Rabiatul Adawiyah Arifin, Nurfadzillah Harun, Nurul Athirah Mohd Azid @ Nazid, Ruwaida Suboh, Ruzira Mohamad Sofian, Nurul Damia Abdul Rahman, Suzanah Abdul Rahim, Muhd Afzam Shah Sha'ban, Munirah Q Science (General) Devoid of scientific proofs, the use of commercially available natural product-based eye toners for therapeutic purposes incites controversies surrounding it. This study aims to evaluate the effect of selected commercially available eye toners on in vitro cellular model using rabbits’ corneal keratocytes. The primary cells were cultivated in culture media supplemented with or without eye toners as control. Growth kinetic profile, cells proliferation assay, scratch assay and immunocytochemistry analysis were performed in comparing both culture media. There were no significant differences between control and the commercially available eye toners in the growth kinetics and cell proliferation. Microscopic observation of the scratch assay suggested the eye toners did not promote cells migration as compare to control. However, in the immunohistochemistry study the eye toners are able to sustain the expression of Connexin-43 and Lumican. This preliminary study indicates that the selected eye toners do not promote the proliferation and migration of corneal keratocytes. They are able to maintain the expression of Connexin-43 and Lumican which might have beneficial effect in corneal transparency. Studies with individual element of the eye toners need to be done. Tissue Engineering and Regenerative Medicine Society of Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47518/1/15_REGENERATIVE_RESEARCH_2015.pdf Mohamad Sukri, Norhamiza and Razali, Rabiatul Adawiyah and Arifin, Nurfadzillah and Harun, Nurul Athirah and Mohd Azid @ Nazid, Ruwaida and Suboh, Ruzira and Mohamad Sofian, Nurul Damia and Abdul Rahman, Suzanah and Abdul Rahim, Muhd Afzam Shah and Sha'ban, Munirah (2015) Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration. Regenerative Research, 4 (1). pp. 13-22. E-ISSN 2232-0822 http://regres.tesma.org.my/pdf/RR-050515-006.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohamad Sukri, Norhamiza Razali, Rabiatul Adawiyah Arifin, Nurfadzillah Harun, Nurul Athirah Mohd Azid @ Nazid, Ruwaida Suboh, Ruzira Mohamad Sofian, Nurul Damia Abdul Rahman, Suzanah Abdul Rahim, Muhd Afzam Shah Sha'ban, Munirah Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration
description	Devoid of scientific proofs, the use of commercially available natural product-based eye toners for therapeutic purposes incites controversies surrounding it. This study aims to evaluate the effect of selected commercially available eye toners on in vitro cellular model using rabbits’ corneal keratocytes. The primary cells were cultivated in culture media supplemented with or without eye toners as control. Growth kinetic profile, cells proliferation assay, scratch assay and immunocytochemistry analysis were performed in comparing both culture media. There were no significant differences between control and the commercially available eye toners in the growth kinetics and cell proliferation. Microscopic observation of the scratch assay suggested the eye toners did not promote cells migration as compare to control. However, in the immunohistochemistry study the eye toners are able to sustain the expression of Connexin-43 and Lumican. This preliminary study indicates that the selected eye toners do not promote the proliferation and migration of corneal keratocytes. They are able to maintain the expression of Connexin-43 and Lumican which might have beneficial effect in corneal transparency. Studies with individual element of the eye toners need to be done.
format	Article
author	Mohamad Sukri, Norhamiza Razali, Rabiatul Adawiyah Arifin, Nurfadzillah Harun, Nurul Athirah Mohd Azid @ Nazid, Ruwaida Suboh, Ruzira Mohamad Sofian, Nurul Damia Abdul Rahman, Suzanah Abdul Rahim, Muhd Afzam Shah Sha'ban, Munirah
author_facet	Mohamad Sukri, Norhamiza Razali, Rabiatul Adawiyah Arifin, Nurfadzillah Harun, Nurul Athirah Mohd Azid @ Nazid, Ruwaida Suboh, Ruzira Mohamad Sofian, Nurul Damia Abdul Rahman, Suzanah Abdul Rahim, Muhd Afzam Shah Sha'ban, Munirah
author_sort	Mohamad Sukri, Norhamiza
title	Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration
title_short	Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration
title_full	Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration
title_fullStr	Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration
title_full_unstemmed	Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration
title_sort	preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration
publisher	Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate	2015
url	http://irep.iium.edu.my/47518/ http://irep.iium.edu.my/47518/ http://irep.iium.edu.my/47518/1/15_REGENERATIVE_RESEARCH_2015.pdf
first_indexed	2023-09-18T21:07:36Z
last_indexed	2023-09-18T21:07:36Z
_version_	1777411038309777408

Preliminary study on the effect of commercially available eye toners on rabbit corneal keratocytes proliferation and migration

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