Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro

Sox9 plays an important role as transcription factor for chondrogenesis; the formation of cartilage. This study aimed to identify the potential of the transiently overexpressed Sox9 gene in human chondrocytes differentiation and tissue engineered cartilage (TEC) formation in vitro. Articular cartila...

Full description

Bibliographic Details
Main Authors:	Mohamad Sukri, Norhamiza, Ahmad Radzi, Muhammad Aa’zamuddin, Abdul Rahman, Rozlin, Zulkifly, Ahmad Hafiz, Abdulahi Hashi, Abdurezak, Sha’ban, Munirah
Format:	Article
Language:	English English
Published:	Universiti Teknologi Malaysia 2015
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/47512/ http://irep.iium.edu.my/47512/ http://irep.iium.edu.my/47512/ http://irep.iium.edu.my/47512/1/07_JT_6748-18532-1-PB%2C_SCOPUS_2015.pdf http://irep.iium.edu.my/47512/4/47512_Identifying_the_potential_of_transcription_factor_sox9_SCOPUS.pdf

id	iium-47512
recordtype	eprints
spelling	iium-475122016-07-15T02:31:52Z http://irep.iium.edu.my/47512/ Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Zulkifly, Ahmad Hafiz Abdulahi Hashi, Abdurezak Sha’ban, Munirah R Medicine (General) Sox9 plays an important role as transcription factor for chondrogenesis; the formation of cartilage. This study aimed to identify the potential of the transiently overexpressed Sox9 gene in human chondrocytes differentiation and tissue engineered cartilage (TEC) formation in vitro. Articular cartilage samples were obtained from osteoarthritic patients who underwent joint replacement surgery. The isolated chondrocytes were cultured and transfected with pcDNA3-Sox9 using lipofection technique. The TEC constructs were formed by incorporating the transfected and the non-transfected cells onto poly(lactic-co-glycolic acid) (PLGA) scaffold with or without fibrin. This approach allows a comparison between four groups i.e. (1) transfected chondrocytes seeded on PLGA/fibrin [PFTC], (2) non-transfected chondrocytes on PLGA/fibrin [PFC], (3) transfected chondrocytes on PLGA [PTC] and (4) non-transfected chondrocytes on PLGA [PC]. All TEC constructs were cultured and evaluated at each time point of 1, 2 and 3 weeks in vitro. All TEC constructs were analysed for gross observation, histology, immunohistochemistry, cell proliferation activity, gene expression and sulphated glycosaminoglycan (sGAG) production assay. After 3 weeks, all PFTC and PFC showed higher cell viability, higher sGAG content, better histological features and distribution of extracellular matrix in concert with positive glycosaminoglycan (GAG) accumulation when compared to the PTC and PC. However, at week 3, the PFC and PC exhibited significantly higher sGAG production than PFTC and PTC. Chondrogenic properties of the constructs were evidenced by the expression of cartilage-specific markers; collagen II, collagen XI and aggrecan core protein. In this study, due to the nature of a new cartilage formation, the co-expression of collagen I in all constructs can be an indication of early cartilage development. Based on the outcomes, it is hoped that this study will provide a good ground for future tissue engineering application. Universiti Teknologi Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47512/1/07_JT_6748-18532-1-PB%2C_SCOPUS_2015.pdf application/pdf en http://irep.iium.edu.my/47512/4/47512_Identifying_the_potential_of_transcription_factor_sox9_SCOPUS.pdf Mohamad Sukri, Norhamiza and Ahmad Radzi, Muhammad Aa’zamuddin and Abdul Rahman, Rozlin and Zulkifly, Ahmad Hafiz and Abdulahi Hashi, Abdurezak and Sha’ban, Munirah (2015) Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro. Jurnal Teknologi, 77 (25). pp. 107-113. ISSN 0127–9696 E-ISSN 2180–3722 http://dx.doi.org/10.11113/jt.v77.6748 10.11113/jt.v77.6748
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Zulkifly, Ahmad Hafiz Abdulahi Hashi, Abdurezak Sha’ban, Munirah Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
description	Sox9 plays an important role as transcription factor for chondrogenesis; the formation of cartilage. This study aimed to identify the potential of the transiently overexpressed Sox9 gene in human chondrocytes differentiation and tissue engineered cartilage (TEC) formation in vitro. Articular cartilage samples were obtained from osteoarthritic patients who underwent joint replacement surgery. The isolated chondrocytes were cultured and transfected with pcDNA3-Sox9 using lipofection technique. The TEC constructs were formed by incorporating the transfected and the non-transfected cells onto poly(lactic-co-glycolic acid) (PLGA) scaffold with or without fibrin. This approach allows a comparison between four groups i.e. (1) transfected chondrocytes seeded on PLGA/fibrin [PFTC], (2) non-transfected chondrocytes on PLGA/fibrin [PFC], (3) transfected chondrocytes on PLGA [PTC] and (4) non-transfected chondrocytes on PLGA [PC]. All TEC constructs were cultured and evaluated at each time point of 1, 2 and 3 weeks in vitro. All TEC constructs were analysed for gross observation, histology, immunohistochemistry, cell proliferation activity, gene expression and sulphated glycosaminoglycan (sGAG) production assay. After 3 weeks, all PFTC and PFC showed higher cell viability, higher sGAG content, better histological features and distribution of extracellular matrix in concert with positive glycosaminoglycan (GAG) accumulation when compared to the PTC and PC. However, at week 3, the PFC and PC exhibited significantly higher sGAG production than PFTC and PTC. Chondrogenic properties of the constructs were evidenced by the expression of cartilage-specific markers; collagen II, collagen XI and aggrecan core protein. In this study, due to the nature of a new cartilage formation, the co-expression of collagen I in all constructs can be an indication of early cartilage development. Based on the outcomes, it is hoped that this study will provide a good ground for future tissue engineering application.
format	Article
author	Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Zulkifly, Ahmad Hafiz Abdulahi Hashi, Abdurezak Sha’ban, Munirah
author_facet	Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa’zamuddin Abdul Rahman, Rozlin Zulkifly, Ahmad Hafiz Abdulahi Hashi, Abdurezak Sha’ban, Munirah
author_sort	Mohamad Sukri, Norhamiza
title	Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
title_short	Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
title_full	Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
title_fullStr	Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
title_full_unstemmed	Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
title_sort	identifying the potential of transcription factor sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/47512/ http://irep.iium.edu.my/47512/ http://irep.iium.edu.my/47512/ http://irep.iium.edu.my/47512/1/07_JT_6748-18532-1-PB%2C_SCOPUS_2015.pdf http://irep.iium.edu.my/47512/4/47512_Identifying_the_potential_of_transcription_factor_sox9_SCOPUS.pdf
first_indexed	2023-09-18T21:07:36Z
last_indexed	2023-09-18T21:07:36Z
_version_	1777411037743546368

Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro

Similar Items