Investigation of in vivo antipyretic activity of thottea dependens leaves

Introduction: Leaves of Thottea dependens have been used as folk medicine in Malaysia for the treatment of several conditions involving pain and inflammation with accompanying pyrexia. However, there is no scientific evidence for its effectiveness to treat fever. Hence, the purpose of this study w...

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Main Authors:	Ahmed, Qamar Uddin, I, Radhiah, Mat So'ad, Siti Zaiton
Format:	Article
Language:	English English English
Published:	IIUM Press 2015
Subjects:	RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/47471/ http://irep.iium.edu.my/47471/ http://irep.iium.edu.my/47471/1/Investigation_of_In_Vivo_Antipyretic_Activity_of.pdf http://irep.iium.edu.my/47471/4/47471_Investigation_of_in_vivo_antipyretic_activity_of_thottea_SCOPUS.pdf http://irep.iium.edu.my/47471/7/47471_Investigation_of_in_vivo_antipyretic_activity_of_thottea_dependens_leaves_latest_copy.pdf

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spelling	iium-474712016-08-16T00:54:58Z http://irep.iium.edu.my/47471/ Investigation of in vivo antipyretic activity of thottea dependens leaves Ahmed, Qamar Uddin I, Radhiah Mat So'ad, Siti Zaiton RM300 Drugs and their action Introduction: Leaves of Thottea dependens have been used as folk medicine in Malaysia for the treatment of several conditions involving pain and inflammation with accompanying pyrexia. However, there is no scientific evidence for its effectiveness to treat fever. Hence, the purpose of this study was to evaluate the anti-pyretic activity of methanol (MeOH) and aqueous (Aq) extracts of T. dependens leaves in albino mice (ICR strain). Methods: Thirty-six albino mice of both sexes, randomized into six groups (n=6) were used. Group I served as the control and received only the vehicle, normal saline. Group II received paracetamol 150 mg/kg body weight (b.w.) orally, groups III and IV received 100 and 200 mg/kg b.w. of the MeOH extract while groups V and VI received 100 and 200 mg/kg b.w. of the Aq extract orally. Pyrexia was induced by a subcutaneous injection of 10 mL/kg b.w. of 15% (w/v) yeast suspended in 0.5% (w/v) methylcellulose solution. LD50 of both extracts were determined according to the method of Organisation for Economic Co-operation and Development guidelines. Phytochemical screening was carried out qualitatively for the presence of different class of biologically active compounds. Results: LD50 values for both extracts were found to be greater than 1000 mg/kg b.w. indicating the wide margin of safety of T. dependens leaves. The MeOH extract at 200 mg/kg b.w. showed statistically significant (P<0.01) dose dependent reduction of brewer’s yeast induced pyrexia in albino mice. Flavonoids, saponins and phenolic compounds were found to be major biologically active phytoconstituents in MeOH extract. Conclusion: The study showed that T. dependens leaves possess significant anti-pyretic activity, thus justifying its ethnomedicinal use in Malaysia to treat fever. KEYWORDS: Thottea dependens, Brewer’s yeast induced pyrexia, albino mice IIUM Press 2015-12-30 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47471/1/Investigation_of_In_Vivo_Antipyretic_Activity_of.pdf application/pdf en http://irep.iium.edu.my/47471/4/47471_Investigation_of_in_vivo_antipyretic_activity_of_thottea_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/47471/7/47471_Investigation_of_in_vivo_antipyretic_activity_of_thottea_dependens_leaves_latest_copy.pdf Ahmed, Qamar Uddin and I, Radhiah and Mat So'ad, Siti Zaiton (2015) Investigation of in vivo antipyretic activity of thottea dependens leaves. The International Medical Journal Malaysia, 14 (2). pp. 11-16. ISSN 1823-4631 http://iiumedic.net/imjm/v1/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RM300 Drugs and their action
spellingShingle	RM300 Drugs and their action Ahmed, Qamar Uddin I, Radhiah Mat So'ad, Siti Zaiton Investigation of in vivo antipyretic activity of thottea dependens leaves
description	Introduction: Leaves of Thottea dependens have been used as folk medicine in Malaysia for the treatment of several conditions involving pain and inflammation with accompanying pyrexia. However, there is no scientific evidence for its effectiveness to treat fever. Hence, the purpose of this study was to evaluate the anti-pyretic activity of methanol (MeOH) and aqueous (Aq) extracts of T. dependens leaves in albino mice (ICR strain). Methods: Thirty-six albino mice of both sexes, randomized into six groups (n=6) were used. Group I served as the control and received only the vehicle, normal saline. Group II received paracetamol 150 mg/kg body weight (b.w.) orally, groups III and IV received 100 and 200 mg/kg b.w. of the MeOH extract while groups V and VI received 100 and 200 mg/kg b.w. of the Aq extract orally. Pyrexia was induced by a subcutaneous injection of 10 mL/kg b.w. of 15% (w/v) yeast suspended in 0.5% (w/v) methylcellulose solution. LD50 of both extracts were determined according to the method of Organisation for Economic Co-operation and Development guidelines. Phytochemical screening was carried out qualitatively for the presence of different class of biologically active compounds. Results: LD50 values for both extracts were found to be greater than 1000 mg/kg b.w. indicating the wide margin of safety of T. dependens leaves. The MeOH extract at 200 mg/kg b.w. showed statistically significant (P<0.01) dose dependent reduction of brewer’s yeast induced pyrexia in albino mice. Flavonoids, saponins and phenolic compounds were found to be major biologically active phytoconstituents in MeOH extract. Conclusion: The study showed that T. dependens leaves possess significant anti-pyretic activity, thus justifying its ethnomedicinal use in Malaysia to treat fever. KEYWORDS: Thottea dependens, Brewer’s yeast induced pyrexia, albino mice
format	Article
author	Ahmed, Qamar Uddin I, Radhiah Mat So'ad, Siti Zaiton
author_facet	Ahmed, Qamar Uddin I, Radhiah Mat So'ad, Siti Zaiton
author_sort	Ahmed, Qamar Uddin
title	Investigation of in vivo antipyretic activity of thottea dependens leaves
title_short	Investigation of in vivo antipyretic activity of thottea dependens leaves
title_full	Investigation of in vivo antipyretic activity of thottea dependens leaves
title_fullStr	Investigation of in vivo antipyretic activity of thottea dependens leaves
title_full_unstemmed	Investigation of in vivo antipyretic activity of thottea dependens leaves
title_sort	investigation of in vivo antipyretic activity of thottea dependens leaves
publisher	IIUM Press
publishDate	2015
url	http://irep.iium.edu.my/47471/ http://irep.iium.edu.my/47471/ http://irep.iium.edu.my/47471/1/Investigation_of_In_Vivo_Antipyretic_Activity_of.pdf http://irep.iium.edu.my/47471/4/47471_Investigation_of_in_vivo_antipyretic_activity_of_thottea_SCOPUS.pdf http://irep.iium.edu.my/47471/7/47471_Investigation_of_in_vivo_antipyretic_activity_of_thottea_dependens_leaves_latest_copy.pdf
first_indexed	2023-09-18T21:07:33Z
last_indexed	2023-09-18T21:07:33Z
_version_	1777411034428997632

Investigation of in vivo antipyretic activity of thottea dependens leaves

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