Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair

Articular cartilage has poor repair capacity due to its avascular and aneural properties and has relatively few cells. This study investigated the ability of autologous implantation approach using three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on...

Full description

Bibliographic Details
Main Authors:	Abdul Rahman, Rozlin, Mohamad Sukri, Norhamiza, Md Nazir, Noorhidayah, Ahmad Radzi, Muhammad Aa'zamuddin, Zulkifly, Ahmad Hafiz, Che Ahmad, Aminudin, Abdul Rahman, Suzanah, Sha'ban, Munirah
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Online Access:	http://irep.iium.edu.my/47426/ http://irep.iium.edu.my/47426/ http://irep.iium.edu.my/47426/ http://irep.iium.edu.my/47426/1/6743-18520-1-SM.pdf

id	iium-47426
recordtype	eprints
spelling	iium-474262016-07-15T02:09:29Z http://irep.iium.edu.my/47426/ Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Sha'ban, Munirah Articular cartilage has poor repair capacity due to its avascular and aneural properties and has relatively few cells. This study investigated the ability of autologous implantation approach using three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. The engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and more intense expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair. Universiti Teknologi Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47426/1/6743-18520-1-SM.pdf Abdul Rahman, Rozlin and Mohamad Sukri, Norhamiza and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Abdul Rahman, Suzanah and Sha'ban, Munirah (2015) Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair. Jurnal Teknologi, 77 (25). pp. 77-82. ISSN 0127–9696 E-ISSN 2180–3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6743 http://dx.doi.org/10.11113/jt.v77.6743
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
description	Articular cartilage has poor repair capacity due to its avascular and aneural properties and has relatively few cells. This study investigated the ability of autologous implantation approach using three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. The engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and more intense expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair.
format	Article
author	Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Sha'ban, Munirah
spellingShingle	Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Sha'ban, Munirah Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
author_facet	Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Md Nazir, Noorhidayah Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Sha'ban, Munirah
author_sort	Abdul Rahman, Rozlin
title	Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
title_short	Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
title_full	Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
title_fullStr	Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
title_full_unstemmed	Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
title_sort	evaluation of three dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/47426/ http://irep.iium.edu.my/47426/ http://irep.iium.edu.my/47426/ http://irep.iium.edu.my/47426/1/6743-18520-1-SM.pdf
first_indexed	2023-09-18T21:07:29Z
last_indexed	2023-09-18T21:07:29Z
_version_	1777411030934093824

Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair

Similar Items