Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation

The high demand of Stevia rebaudiana, commonly known as Stevia encourages people for commercial-scale cultivation. In vitro clonal propagation is a potential alternative for conventional propagation due to its advantages. However, microbial contamination is a constant problem for in vitro clonal pro...

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Main Authors:	Urbi, Zannat, Zainuddin, Zarina
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Online Access:	http://irep.iium.edu.my/47417/ http://irep.iium.edu.my/47417/ http://irep.iium.edu.my/47417/ http://irep.iium.edu.my/47417/1/6722-18468-1-SM.pdf

id	iium-47417
recordtype	eprints
spelling	iium-474172018-05-23T00:28:42Z http://irep.iium.edu.my/47417/ Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation Urbi, Zannat Zainuddin, Zarina The high demand of Stevia rebaudiana, commonly known as Stevia encourages people for commercial-scale cultivation. In vitro clonal propagation is a potential alternative for conventional propagation due to its advantages. However, microbial contamination is a constant problem for in vitro clonal propagation of field grown plant. Therefore, the aim of this study was to establish an effective surface sterilization protocol for Stevia prior to in vitro propagation. Two disinfecting agents, NaOCl and HgCl2 with different concentrations and various exposure times were tested for surface sterilization of shoot tips and nodal explants. Results show that treatment of explants with NaOCl was more effective compared to HgCl2 and 5% NaOCl for 10 minutes with 0.2% carbendazim (fungicide) pre-treatment showed least contamination and highest survival rate. Fungal contamination was observed to be a main problem in this study and it occurred within 2-3 days of inoculation, leading to the death of the explants. Universiti Teknologi Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47417/1/6722-18468-1-SM.pdf Urbi, Zannat and Zainuddin, Zarina (2015) Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation. Jurnal Teknologi, 77 (24). pp. 141-146. ISSN 2180–3722 (O), 0127–9696 (P) http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6722 http://dx.doi.org/10.11113/jt.v77.6722
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
description	The high demand of Stevia rebaudiana, commonly known as Stevia encourages people for commercial-scale cultivation. In vitro clonal propagation is a potential alternative for conventional propagation due to its advantages. However, microbial contamination is a constant problem for in vitro clonal propagation of field grown plant. Therefore, the aim of this study was to establish an effective surface sterilization protocol for Stevia prior to in vitro propagation. Two disinfecting agents, NaOCl and HgCl2 with different concentrations and various exposure times were tested for surface sterilization of shoot tips and nodal explants. Results show that treatment of explants with NaOCl was more effective compared to HgCl2 and 5% NaOCl for 10 minutes with 0.2% carbendazim (fungicide) pre-treatment showed least contamination and highest survival rate. Fungal contamination was observed to be a main problem in this study and it occurred within 2-3 days of inoculation, leading to the death of the explants.
format	Article
author	Urbi, Zannat Zainuddin, Zarina
spellingShingle	Urbi, Zannat Zainuddin, Zarina Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation
author_facet	Urbi, Zannat Zainuddin, Zarina
author_sort	Urbi, Zannat
title	Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation
title_short	Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation
title_full	Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation
title_fullStr	Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation
title_full_unstemmed	Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation
title_sort	standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/47417/ http://irep.iium.edu.my/47417/ http://irep.iium.edu.my/47417/ http://irep.iium.edu.my/47417/1/6722-18468-1-SM.pdf
first_indexed	2023-09-18T21:07:29Z
last_indexed	2023-09-18T21:07:29Z
_version_	1777411030236790784

Standardization of surface sterilization protocol of field grown stevia rebaudiana prior to in vitro clonal propagation

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