Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes

Introduction: Obesity is the major health problem both in developing and developed countries and Malaysia is no exception. Due to obesity, various adverse health conditions such as diabetes mellitus (DM), metabolic syndrome (MS) and cardiovascular diseases (CVDs) are frequently inborn. The prevalenc...

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Main Authors:	Sassi, Assia, Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/47393/ http://irep.iium.edu.my/47393/ http://irep.iium.edu.my/47393/1/Document1.pdf

id	iium-47393
recordtype	eprints
spelling	iium-473932018-05-21T06:52:07Z http://irep.iium.edu.my/47393/ Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher RS Pharmacy and materia medica Introduction: Obesity is the major health problem both in developing and developed countries and Malaysia is no exception. Due to obesity, various adverse health conditions such as diabetes mellitus (DM), metabolic syndrome (MS) and cardiovascular diseases (CVDs) are frequently inborn. The prevalence’s of the listed diseases are increasing at alarmingly higher rates in the world including Malaysia. The treatment cost is increasing exponentially therefore; prevention would be the better option to fight against these diseases. Materials and Methods: Three common vegetables namely Momordica Charantia (also known as Bitter gourd, Karela or Peria Katak), LagenariaSiceraria (known as Bottle Gourd, Calabash) and TrichosanthesCucumerina (known as Snake gourd or LabuUlar) of the Cucurbitaceae family were investigated for their water and ethanolic extracts (extracted from whole vegetable, peels and seeds) effect on 3T3-L1 adipocytes were assessed on cell differentiation, adipogenesis, adipolysis (using appropriate kits) and on glucose uptakes (assessed by radioisotopes). Results: The cells tolerated a concentration up to 0.063 mg/ml of extracts. The cell upon treatment demonstrated reduced lipid accumulation in the cells. The water and ethanol extracts showed reduction in lipid accumulation which is evident from reduced adipogenesis in the treated cells. Bitter gourd and Snake gourd seeds and Bottle Gourd peels & seeds of the ethanolic extract showed highest inhibition/reduction of adipogenesis. Lipolysis was enhanced when the adipocytes were treated with ethanolic extracts of the whole and peel from the three sources of vegetables. Furthermore, there was improved glucose uptake in the extract treated cells. Conclusion: The results of this study suggest that these vegetable can be useful for the prevention of obesity and also may provide better regulation over glucose metabolism. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/47393/1/Document1.pdf Sassi, Assia and Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher (2015) Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes. In: MASO 2015 Scientific Conference on Obesity, 28th-29th Oct. 2015, Seri Pacific Hotel, Kuala Lumpur. (Unpublished) http://www.maso.org.my/download/MASO2015scentificprogramme.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes
description	Introduction: Obesity is the major health problem both in developing and developed countries and Malaysia is no exception. Due to obesity, various adverse health conditions such as diabetes mellitus (DM), metabolic syndrome (MS) and cardiovascular diseases (CVDs) are frequently inborn. The prevalence’s of the listed diseases are increasing at alarmingly higher rates in the world including Malaysia. The treatment cost is increasing exponentially therefore; prevention would be the better option to fight against these diseases. Materials and Methods: Three common vegetables namely Momordica Charantia (also known as Bitter gourd, Karela or Peria Katak), LagenariaSiceraria (known as Bottle Gourd, Calabash) and TrichosanthesCucumerina (known as Snake gourd or LabuUlar) of the Cucurbitaceae family were investigated for their water and ethanolic extracts (extracted from whole vegetable, peels and seeds) effect on 3T3-L1 adipocytes were assessed on cell differentiation, adipogenesis, adipolysis (using appropriate kits) and on glucose uptakes (assessed by radioisotopes). Results: The cells tolerated a concentration up to 0.063 mg/ml of extracts. The cell upon treatment demonstrated reduced lipid accumulation in the cells. The water and ethanol extracts showed reduction in lipid accumulation which is evident from reduced adipogenesis in the treated cells. Bitter gourd and Snake gourd seeds and Bottle Gourd peels & seeds of the ethanolic extract showed highest inhibition/reduction of adipogenesis. Lipolysis was enhanced when the adipocytes were treated with ethanolic extracts of the whole and peel from the three sources of vegetables. Furthermore, there was improved glucose uptake in the extract treated cells. Conclusion: The results of this study suggest that these vegetable can be useful for the prevention of obesity and also may provide better regulation over glucose metabolism.
format	Conference or Workshop Item
author	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_facet	Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher
author_sort	Sassi, Assia
title	Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes
title_short	Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes
title_full	Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes
title_fullStr	Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes
title_full_unstemmed	Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes
title_sort	effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes
publishDate	2015
url	http://irep.iium.edu.my/47393/ http://irep.iium.edu.my/47393/ http://irep.iium.edu.my/47393/1/Document1.pdf
first_indexed	2023-09-18T21:07:27Z
last_indexed	2023-09-18T21:07:27Z
_version_	1777411028080918528

Effect of common vegetables extracts on lipid and glucose in 3t3-l1 adipocytes

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