DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene

DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene

This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or white threads fis...

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Main Authors: K., Kamarudin, Mohamed Rehan, Maryam, Ramly, Nur Zazarina, Mohamed Rehan, Aisyah
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/47379/
http://irep.iium.edu.my/47379/
http://irep.iium.edu.my/47379/1/43579.pdf
id iium-47379
recordtype eprints
spelling iium-473792016-05-24T05:26:32Z http://irep.iium.edu.my/47379/ DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene K., Kamarudin Mohamed Rehan, Maryam Ramly, Nur Zazarina Mohamed Rehan, Aisyah Q Science (General) This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or white threads fish from Pangkor Island, Perak Darul Ridzuan, Malaysia applying partial DNA sequencing of non-protein-coding 16S mitochondrial ribosomal RNA gene. Pangkor Island in the north region of Peninsular Malaysia is well known for its traditional production industry of air gamat (body fluid extracts) and minyak gamat (lipid extracts). The phylogenetic analyses using Neighbour-Joining method and Maximum Likelihood method supported the morphospecies status of H. leucospilota and S. horrens. Despite the fact that more specimens and different molecular approaches are required to produce better conclusive results, the current findings gave a better insight of the importance of morphological approach and 16S mitochondrial rRNA gene sequencing in identification of the two Malaysian sea cucumber species from Pangkor Island. Furthermore, the gene barcodes can be used as the commercial species standard or reference samples in determining the species identities of processed sea cucumbers from Malaysian markets. 2015-10-13 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47379/1/43579.pdf K., Kamarudin and Mohamed Rehan, Maryam and Ramly, Nur Zazarina and Mohamed Rehan, Aisyah (2015) DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene. In: Kolokium Siswazah Sains dan Teknologi (KoSiST'15), 13th Oct. 2015, Akademi Kepimpinan Pengajian Tinggi (AKEPT), Bandar Enstek, Negeri Sembilan. http://pcfst.usim.edu.my/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
spellingShingle Q Science (General)
K., Kamarudin
Mohamed Rehan, Maryam
Ramly, Nur Zazarina
Mohamed Rehan, Aisyah
DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
description This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or white threads fish from Pangkor Island, Perak Darul Ridzuan, Malaysia applying partial DNA sequencing of non-protein-coding 16S mitochondrial ribosomal RNA gene. Pangkor Island in the north region of Peninsular Malaysia is well known for its traditional production industry of air gamat (body fluid extracts) and minyak gamat (lipid extracts). The phylogenetic analyses using Neighbour-Joining method and Maximum Likelihood method supported the morphospecies status of H. leucospilota and S. horrens. Despite the fact that more specimens and different molecular approaches are required to produce better conclusive results, the current findings gave a better insight of the importance of morphological approach and 16S mitochondrial rRNA gene sequencing in identification of the two Malaysian sea cucumber species from Pangkor Island. Furthermore, the gene barcodes can be used as the commercial species standard or reference samples in determining the species identities of processed sea cucumbers from Malaysian markets.
format Conference or Workshop Item
author K., Kamarudin
Mohamed Rehan, Maryam
Ramly, Nur Zazarina
Mohamed Rehan, Aisyah
author_facet K., Kamarudin
Mohamed Rehan, Maryam
Ramly, Nur Zazarina
Mohamed Rehan, Aisyah
author_sort K., Kamarudin
title DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
title_short DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
title_full DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
title_fullStr DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
title_full_unstemmed DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
title_sort dna barcoding of gamat and timun laut species from pangkor island using 16s mitochondrial ribosomal rna gene
publishDate 2015
url http://irep.iium.edu.my/47379/
http://irep.iium.edu.my/47379/
http://irep.iium.edu.my/47379/1/43579.pdf
first_indexed 2023-09-18T21:07:25Z
last_indexed 2023-09-18T21:07:25Z
_version_ 1777411026499665920

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