Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice

The polyamine biosynthesis pathway plays a significant role in cell growth, both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accord...

Full description

Bibliographic Details
Main Authors:	Abdul Ghani, Radiah, Nik Abdul Malek, Nik Nurasyikin, Md Yusof, Najwa Farhah, Jamil, Elyna Fatini
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Online Access:	http://irep.iium.edu.my/47360/ http://irep.iium.edu.my/47360/ http://irep.iium.edu.my/47360/ http://irep.iium.edu.my/47360/1/6746-18526-1-SM.pdf

id	iium-47360
recordtype	eprints
spelling	iium-473602016-07-15T01:32:33Z http://irep.iium.edu.my/47360/ Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice Abdul Ghani, Radiah Nik Abdul Malek, Nik Nurasyikin Md Yusof, Najwa Farhah Jamil, Elyna Fatini The polyamine biosynthesis pathway plays a significant role in cell growth, both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. The inhibition of growth was determined using Trypan Blue exclusion and the changes in intracellular polyamine in pomegranate treated cells was observed using High Performance Liquid Chromatography (HPLC). It was found that there was inhibition of A549 cell growth and reduced in intracellular polyamine content in pomegranate treated cells. The ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism. Universiti Teknologi Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47360/1/6746-18526-1-SM.pdf Abdul Ghani, Radiah and Nik Abdul Malek, Nik Nurasyikin and Md Yusof, Najwa Farhah and Jamil, Elyna Fatini (2015) Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice. Jurnal Teknologi, 77 (25). pp. 95-99. ISSN 0127–9696 E-ISSN 2180–3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6746 http://dx.doi.org/10.11113/jt.v77.6746
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
description	The polyamine biosynthesis pathway plays a significant role in cell growth, both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. The inhibition of growth was determined using Trypan Blue exclusion and the changes in intracellular polyamine in pomegranate treated cells was observed using High Performance Liquid Chromatography (HPLC). It was found that there was inhibition of A549 cell growth and reduced in intracellular polyamine content in pomegranate treated cells. The ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism.
format	Article
author	Abdul Ghani, Radiah Nik Abdul Malek, Nik Nurasyikin Md Yusof, Najwa Farhah Jamil, Elyna Fatini
spellingShingle	Abdul Ghani, Radiah Nik Abdul Malek, Nik Nurasyikin Md Yusof, Najwa Farhah Jamil, Elyna Fatini Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice
author_facet	Abdul Ghani, Radiah Nik Abdul Malek, Nik Nurasyikin Md Yusof, Najwa Farhah Jamil, Elyna Fatini
author_sort	Abdul Ghani, Radiah
title	Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice
title_short	Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice
title_full	Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice
title_fullStr	Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice
title_full_unstemmed	Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice
title_sort	ornithine decarboxylase gene expression in human lung adenocarcinoma cell (a549) treated with pomegranate juice
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/47360/ http://irep.iium.edu.my/47360/ http://irep.iium.edu.my/47360/ http://irep.iium.edu.my/47360/1/6746-18526-1-SM.pdf
first_indexed	2023-09-18T21:07:24Z
last_indexed	2023-09-18T21:07:24Z
_version_	1777411025366155264

Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice

Similar Items