A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture

This study aimed at establishing callus cultures as an alternative research tool to study the regulatory mechanisms of the carotenoid biosynthesis in plant through a controlled environment. Previous studies established that selasih or O. basilicum is rich with carotenoids such as neoxanthin, lutein...

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Main Authors:	Othman, Rashidi, Mohd Zaifuddin, Fatimah Azzahra, Mohd Hassan, Norazian
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/47247/ http://irep.iium.edu.my/47247/ http://irep.iium.edu.my/47247/1/PAPER4.pdf

id	iium-47247
recordtype	eprints
spelling	iium-472472018-05-21T01:14:13Z http://irep.iium.edu.my/47247/ A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology This study aimed at establishing callus cultures as an alternative research tool to study the regulatory mechanisms of the carotenoid biosynthesis in plant through a controlled environment. Previous studies established that selasih or O. basilicum is rich with carotenoids such as neoxanthin, lutein, violaxanthin, α-carotene and ß-carotene. Interestingly other researcher found selasih was rich with high amount of zeaxanthin. Therefore the question remain in this study is what are the key factors regulate carotenoid accumulation mechanism and how far the extent of environmental and genetic influences on plant carotenoid biosynthesis. In this study carotenoid biogenesis will be investigated in selasih callus, pumpkin and Geroda cultures as a potential model system for rapid initiation, extraction and analysis of carotenoids by providing stringent control of genetic, developmental and environmental factors. The value of this experimental system for investigating variables controlling carotenoid accumulation is then tested by assessing the effects of environmental variables, such as drought stress, light intensity and water stress on carotenoid accumulation. Reversed phase high-performance liquid chromatography (HPLC) analysis established that light, salicylic acid and PEG influenced the presence of lutein and β-carotene. Although these factors had an effect on the carotenoid content and profile, the most influential factor appeared to be plant species selection. This study is expected to provide new knowledge on the key factors involved in regulatory mechanisms of individual carotenoid biosynthesis in a particular biology system of plant. On top of that, future exploration and manipulation of the genetic make-up is made possible to enhance or enrich certain individual carotenoid in food crops without altering the genes or genetic modification. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47247/1/PAPER4.pdf Othman, Rashidi and Mohd Zaifuddin, Fatimah Azzahra and Mohd Hassan, Norazian (2015) A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture. In: Asian Congress on Biotechnology 2015 (ACB 2015), 15th-19th Nov 2015, Istana Hotel, Kuala Lumpur. (Unpublished) http://www.acb2015.my/web/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology
spellingShingle	RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture
description	This study aimed at establishing callus cultures as an alternative research tool to study the regulatory mechanisms of the carotenoid biosynthesis in plant through a controlled environment. Previous studies established that selasih or O. basilicum is rich with carotenoids such as neoxanthin, lutein, violaxanthin, α-carotene and ß-carotene. Interestingly other researcher found selasih was rich with high amount of zeaxanthin. Therefore the question remain in this study is what are the key factors regulate carotenoid accumulation mechanism and how far the extent of environmental and genetic influences on plant carotenoid biosynthesis. In this study carotenoid biogenesis will be investigated in selasih callus, pumpkin and Geroda cultures as a potential model system for rapid initiation, extraction and analysis of carotenoids by providing stringent control of genetic, developmental and environmental factors. The value of this experimental system for investigating variables controlling carotenoid accumulation is then tested by assessing the effects of environmental variables, such as drought stress, light intensity and water stress on carotenoid accumulation. Reversed phase high-performance liquid chromatography (HPLC) analysis established that light, salicylic acid and PEG influenced the presence of lutein and β-carotene. Although these factors had an effect on the carotenoid content and profile, the most influential factor appeared to be plant species selection. This study is expected to provide new knowledge on the key factors involved in regulatory mechanisms of individual carotenoid biosynthesis in a particular biology system of plant. On top of that, future exploration and manipulation of the genetic make-up is made possible to enhance or enrich certain individual carotenoid in food crops without altering the genes or genetic modification.
format	Conference or Workshop Item
author	Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian
author_facet	Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian
author_sort	Othman, Rashidi
title	A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture
title_short	A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture
title_full	A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture
title_fullStr	A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture
title_full_unstemmed	A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture
title_sort	high-throughput model system for investigating carotenoid biogenesis in plant via callus culture
publishDate	2015
url	http://irep.iium.edu.my/47247/ http://irep.iium.edu.my/47247/ http://irep.iium.edu.my/47247/1/PAPER4.pdf
first_indexed	2023-09-18T21:07:15Z
last_indexed	2023-09-18T21:07:15Z
_version_	1777411015697235968

A high-throughput model system for investigating carotenoid biogenesis in plant via callus culture

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