Low-cost microcontact printing for direct enzyme patterning on paper

In this study, an easy, low-cost, and straightforward method to pattern and directly control the amount of enzyme deposited on paper using microcontact printing was successfully developed without the need for a cleanroom facility. Full characterization was done to evaluate the effectiveness of this...

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Main Authors:	Ahmad, Siti Khadijah, Arris, Farrah Aida, Wan Salim, Wan Wardatul Amani
Format:	Conference or Workshop Item
Language:	English English English English
Published:	2015
Subjects:	T175 Industrial research. Research and development
Online Access:	http://irep.iium.edu.my/47228/ http://irep.iium.edu.my/47228/4/ACB_Low_cost_microcontact_pritning_SKA_WWAWS_ver_3.pdf http://irep.iium.edu.my/47228/1/ACB2015_SitiKhadijah_Farrah_Aida_Arris_Dr_Wan_W_Amani_Final.pdf http://irep.iium.edu.my/47228/5/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim_Siti_Khadijah.pdf http://irep.iium.edu.my/47228/6/ACB_2015_Farrah_Mai.pdf

id	iium-47228
recordtype	eprints
spelling	iium-472282016-05-23T23:50:42Z http://irep.iium.edu.my/47228/ Low-cost microcontact printing for direct enzyme patterning on paper Ahmad, Siti Khadijah Arris, Farrah Aida Wan Salim, Wan Wardatul Amani T175 Industrial research. Research and development In this study, an easy, low-cost, and straightforward method to pattern and directly control the amount of enzyme deposited on paper using microcontact printing was successfully developed without the need for a cleanroom facility. Full characterization was done to evaluate the effectiveness of this approach, from the fabrication of a master stamp with controlled width spacing of 20, 50, and 100 μm using a vertical micromilling method to the stamping of glucose oxidase (GOx) onto papers with different porosity. To prove the concept of direct enzyme patterning, GOx is patterned on paper impregnated with anthocyanin, a water-soluble vacuolar pigment. Anthocyanin contains glucose covalently attached to the flavonoid backbone and is able to give a colorimetric change from purple to red to indicate successful deposition of GOx owing to the product of gluconic acid. Stamping process parameters were varied in terms of force (8, 20, 40, and 50 g) and time (5, 30, and 60 s) to transfer GOx from soft stamp to porous paper. Results show that the paper with the highest porosity stamped using a 200 g load for 5 s, gives the best result in terms of patterns and successful transfer of enzyme onto the paper. This study suggests that the fabrication of a master stamp using inexpensive micromilling methods can produce a soft stamper that can pattern a bioactive enzyme. 2015-12-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47228/4/ACB_Low_cost_microcontact_pritning_SKA_WWAWS_ver_3.pdf application/pdf en http://irep.iium.edu.my/47228/1/ACB2015_SitiKhadijah_Farrah_Aida_Arris_Dr_Wan_W_Amani_Final.pdf application/pdf en http://irep.iium.edu.my/47228/5/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim_Siti_Khadijah.pdf application/pdf en http://irep.iium.edu.my/47228/6/ACB_2015_Farrah_Mai.pdf Ahmad, Siti Khadijah and Arris, Farrah Aida and Wan Salim, Wan Wardatul Amani (2015) Low-cost microcontact printing for direct enzyme patterning on paper. In: 12th Asian Congress on Biotechnology (ACB 2015) , 15th-19th November 2015, Istana Hotel, Kuala Lumpur, Malaysia..
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English English
topic	T175 Industrial research. Research and development
spellingShingle	T175 Industrial research. Research and development Ahmad, Siti Khadijah Arris, Farrah Aida Wan Salim, Wan Wardatul Amani Low-cost microcontact printing for direct enzyme patterning on paper
description	In this study, an easy, low-cost, and straightforward method to pattern and directly control the amount of enzyme deposited on paper using microcontact printing was successfully developed without the need for a cleanroom facility. Full characterization was done to evaluate the effectiveness of this approach, from the fabrication of a master stamp with controlled width spacing of 20, 50, and 100 μm using a vertical micromilling method to the stamping of glucose oxidase (GOx) onto papers with different porosity. To prove the concept of direct enzyme patterning, GOx is patterned on paper impregnated with anthocyanin, a water-soluble vacuolar pigment. Anthocyanin contains glucose covalently attached to the flavonoid backbone and is able to give a colorimetric change from purple to red to indicate successful deposition of GOx owing to the product of gluconic acid. Stamping process parameters were varied in terms of force (8, 20, 40, and 50 g) and time (5, 30, and 60 s) to transfer GOx from soft stamp to porous paper. Results show that the paper with the highest porosity stamped using a 200 g load for 5 s, gives the best result in terms of patterns and successful transfer of enzyme onto the paper. This study suggests that the fabrication of a master stamp using inexpensive micromilling methods can produce a soft stamper that can pattern a bioactive enzyme.
format	Conference or Workshop Item
author	Ahmad, Siti Khadijah Arris, Farrah Aida Wan Salim, Wan Wardatul Amani
author_facet	Ahmad, Siti Khadijah Arris, Farrah Aida Wan Salim, Wan Wardatul Amani
author_sort	Ahmad, Siti Khadijah
title	Low-cost microcontact printing for direct enzyme patterning on paper
title_short	Low-cost microcontact printing for direct enzyme patterning on paper
title_full	Low-cost microcontact printing for direct enzyme patterning on paper
title_fullStr	Low-cost microcontact printing for direct enzyme patterning on paper
title_full_unstemmed	Low-cost microcontact printing for direct enzyme patterning on paper
title_sort	low-cost microcontact printing for direct enzyme patterning on paper
publishDate	2015
url	http://irep.iium.edu.my/47228/ http://irep.iium.edu.my/47228/4/ACB_Low_cost_microcontact_pritning_SKA_WWAWS_ver_3.pdf http://irep.iium.edu.my/47228/1/ACB2015_SitiKhadijah_Farrah_Aida_Arris_Dr_Wan_W_Amani_Final.pdf http://irep.iium.edu.my/47228/5/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim_Siti_Khadijah.pdf http://irep.iium.edu.my/47228/6/ACB_2015_Farrah_Mai.pdf
first_indexed	2023-09-18T21:07:13Z
last_indexed	2023-09-18T21:07:13Z
_version_	1777411013815042048

Low-cost microcontact printing for direct enzyme patterning on paper

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