Electrochemical characterization of graphene nanocomposites for development of robust biosensor

In this study, reduced graphene oxide (rGO) conjugated with gold nanoparticles (AuNPs) in cellulose-nanofiber (CN) matrices, denoted as rGO-AuNP/CN nanocomposite, was characterized via electrochemical methods. The nanocomposite functions as a transducer layer for a flexible and environmentally frien...

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Main Authors:	Arris, Farrah Aida, Ahmad, Siti Khadijah, Wan Salim, Wan Wardatul Amani
Format:	Conference or Workshop Item
Language:	English English English English
Published:	2015
Subjects:	QD Chemistry TP250 Industrial electrochemistry
Online Access:	http://irep.iium.edu.my/47227/ http://irep.iium.edu.my/47227/1/ACB2015_Abstract_Farrah_Aida_Arris_Habibah_Farhana_Dr_Wan_Wardatul_Amani_Wan_Salim_final.pdf http://irep.iium.edu.my/47227/4/ACB_Poster_2015_FAA_WWAWS_Ver_2.pdf http://irep.iium.edu.my/47227/5/ACB_2015_Farrah_Mai.pdf http://irep.iium.edu.my/47227/6/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim_Farrah.pdf

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spelling	iium-472272018-06-12T06:52:45Z http://irep.iium.edu.my/47227/ Electrochemical characterization of graphene nanocomposites for development of robust biosensor Arris, Farrah Aida Ahmad, Siti Khadijah Wan Salim, Wan Wardatul Amani QD Chemistry TP250 Industrial electrochemistry In this study, reduced graphene oxide (rGO) conjugated with gold nanoparticles (AuNPs) in cellulose-nanofiber (CN) matrices, denoted as rGO-AuNP/CN nanocomposite, was characterized via electrochemical methods. The nanocomposite functions as a transducer layer for a flexible and environmentally friendly flexible glucose sensor. The rGO-AuNPs/CN nanocomposite transducer layer was fabricated via an easy two-step physical and subsequent electrochemical deposition method. Surface morphology of the transducer layer was investigated via scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) was used to identify molecule fingerprints of the nanocomposites. Cyclic voltammetry was used to characterize redox capability of the transducer layer. Glucose oxidase (GOx) was subsequently immobilized on the optimized transducer layer for glucose sensing. The best electroactive surface area was determined according to Randles-Sevcik method, where surface current density of 17.3  0.5 μA mM-1 cm-2 was achieved. The optimized rGO-AuNP/CN-TMOS-GOx exhibits amperometric sensitivity of 1350  15.5 nA mM-1, selectivity of 8.1% and 9.9% over 1mM glucose solution for ascorbic acid and acetaminophen respectively, with a detection limit as low as 2.9 μM. The glucose sensor also shows a good linear response range of 0.0032 to 15 mM and good stability with only a 25% loss of enzyme activity after 30 days. All experiments were performed at ambient temperature in the range of 29 to 32oC. 2015-11-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47227/1/ACB2015_Abstract_Farrah_Aida_Arris_Habibah_Farhana_Dr_Wan_Wardatul_Amani_Wan_Salim_final.pdf application/pdf en http://irep.iium.edu.my/47227/4/ACB_Poster_2015_FAA_WWAWS_Ver_2.pdf application/pdf en http://irep.iium.edu.my/47227/5/ACB_2015_Farrah_Mai.pdf application/pdf en http://irep.iium.edu.my/47227/6/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim_Farrah.pdf Arris, Farrah Aida and Ahmad, Siti Khadijah and Wan Salim, Wan Wardatul Amani (2015) Electrochemical characterization of graphene nanocomposites for development of robust biosensor. In: 12th Asian Congress on Biotechnology (ACB 2015) , 15-19 November 2015, Hotel Istana, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English English
topic	QD Chemistry TP250 Industrial electrochemistry
spellingShingle	QD Chemistry TP250 Industrial electrochemistry Arris, Farrah Aida Ahmad, Siti Khadijah Wan Salim, Wan Wardatul Amani Electrochemical characterization of graphene nanocomposites for development of robust biosensor
description	In this study, reduced graphene oxide (rGO) conjugated with gold nanoparticles (AuNPs) in cellulose-nanofiber (CN) matrices, denoted as rGO-AuNP/CN nanocomposite, was characterized via electrochemical methods. The nanocomposite functions as a transducer layer for a flexible and environmentally friendly flexible glucose sensor. The rGO-AuNPs/CN nanocomposite transducer layer was fabricated via an easy two-step physical and subsequent electrochemical deposition method. Surface morphology of the transducer layer was investigated via scanning electron microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) was used to identify molecule fingerprints of the nanocomposites. Cyclic voltammetry was used to characterize redox capability of the transducer layer. Glucose oxidase (GOx) was subsequently immobilized on the optimized transducer layer for glucose sensing. The best electroactive surface area was determined according to Randles-Sevcik method, where surface current density of 17.3  0.5 μA mM-1 cm-2 was achieved. The optimized rGO-AuNP/CN-TMOS-GOx exhibits amperometric sensitivity of 1350  15.5 nA mM-1, selectivity of 8.1% and 9.9% over 1mM glucose solution for ascorbic acid and acetaminophen respectively, with a detection limit as low as 2.9 μM. The glucose sensor also shows a good linear response range of 0.0032 to 15 mM and good stability with only a 25% loss of enzyme activity after 30 days. All experiments were performed at ambient temperature in the range of 29 to 32oC.
format	Conference or Workshop Item
author	Arris, Farrah Aida Ahmad, Siti Khadijah Wan Salim, Wan Wardatul Amani
author_facet	Arris, Farrah Aida Ahmad, Siti Khadijah Wan Salim, Wan Wardatul Amani
author_sort	Arris, Farrah Aida
title	Electrochemical characterization of graphene nanocomposites for development of robust biosensor
title_short	Electrochemical characterization of graphene nanocomposites for development of robust biosensor
title_full	Electrochemical characterization of graphene nanocomposites for development of robust biosensor
title_fullStr	Electrochemical characterization of graphene nanocomposites for development of robust biosensor
title_full_unstemmed	Electrochemical characterization of graphene nanocomposites for development of robust biosensor
title_sort	electrochemical characterization of graphene nanocomposites for development of robust biosensor
publishDate	2015
url	http://irep.iium.edu.my/47227/ http://irep.iium.edu.my/47227/1/ACB2015_Abstract_Farrah_Aida_Arris_Habibah_Farhana_Dr_Wan_Wardatul_Amani_Wan_Salim_final.pdf http://irep.iium.edu.my/47227/4/ACB_Poster_2015_FAA_WWAWS_Ver_2.pdf http://irep.iium.edu.my/47227/5/ACB_2015_Farrah_Mai.pdf http://irep.iium.edu.my/47227/6/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim_Farrah.pdf
first_indexed	2023-09-18T21:07:13Z
last_indexed	2023-09-18T21:07:13Z
_version_	1777411013665095680

Electrochemical characterization of graphene nanocomposites for development of robust biosensor

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