Electrochemical sensor centrifuge platform for single-cell study

SporeSat – a lab-on-a-chip (LOC) centrifuge platform designed for integration as the payload of a small (5.5 kg), free-flying satellite – has been developed to determine the gravitational thresholds for calcium-ion channel activation of a single-cell spore from the fern Ceratopteris richardii. This...

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Main Author:	Wan Salim, Wan Wardatul Amani
Format:	Conference or Workshop Item
Language:	English English English English
Published:	2015
Subjects:	S Agriculture (General) TP248.13 Biotechnology TP250 Industrial electrochemistry
Online Access:	http://irep.iium.edu.my/47224/ http://irep.iium.edu.my/47224/1/ACB2015_Abstract_Dr_Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/4/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/5/ACB_2015_Dr_Amani.pdf http://irep.iium.edu.my/47224/6/ACB_2015_presentation.pdf

id	iium-47224
recordtype	eprints
spelling	iium-472242016-05-20T06:29:36Z http://irep.iium.edu.my/47224/ Electrochemical sensor centrifuge platform for single-cell study Wan Salim, Wan Wardatul Amani S Agriculture (General) TP248.13 Biotechnology TP250 Industrial electrochemistry SporeSat – a lab-on-a-chip (LOC) centrifuge platform designed for integration as the payload of a small (5.5 kg), free-flying satellite – has been developed to determine the gravitational thresholds for calcium-ion channel activation of a single-cell spore from the fern Ceratopteris richardii. This fern is an important model system for gravity-directed plant-cell development during variable-gravity conditions attainable only in space flight. Calcium-ion channel activity is measured by photolithographically defined calcium ion–selective electrodes (ISEs) at opposite ends of each spore. Artificial gravity is created by rotating a disk-like platform that contains the spores in wells along with the calcium ISEs. Ground experiments reveal a maximum calcium concentration ratio at 2.2xg, between micro-ion-selective electrodes near the “top” and “bottom” ends of the spore, indicating an increasing calcium concentration at one “end” of the fern spore with respect to the other. Confocal micrographs of rhizoid formation confirm the light-induced germination. SporeSat is a spaceflight experiment that will take ~ 4 days; data will be telemetered to Earth over ~ 100 days. 2015-11-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/47224/1/ACB2015_Abstract_Dr_Wan_Wardatul_Amani_Wan_Salim.pdf application/pdf en http://irep.iium.edu.my/47224/4/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim.pdf application/pdf en http://irep.iium.edu.my/47224/5/ACB_2015_Dr_Amani.pdf application/pdf en http://irep.iium.edu.my/47224/6/ACB_2015_presentation.pdf Wan Salim, Wan Wardatul Amani (2015) Electrochemical sensor centrifuge platform for single-cell study. In: 12th Asian Congress on Biotechnology (ACB 2015) , 15-19 November 2015, Hotel Istana, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English English
topic	S Agriculture (General) TP248.13 Biotechnology TP250 Industrial electrochemistry
spellingShingle	S Agriculture (General) TP248.13 Biotechnology TP250 Industrial electrochemistry Wan Salim, Wan Wardatul Amani Electrochemical sensor centrifuge platform for single-cell study
description	SporeSat – a lab-on-a-chip (LOC) centrifuge platform designed for integration as the payload of a small (5.5 kg), free-flying satellite – has been developed to determine the gravitational thresholds for calcium-ion channel activation of a single-cell spore from the fern Ceratopteris richardii. This fern is an important model system for gravity-directed plant-cell development during variable-gravity conditions attainable only in space flight. Calcium-ion channel activity is measured by photolithographically defined calcium ion–selective electrodes (ISEs) at opposite ends of each spore. Artificial gravity is created by rotating a disk-like platform that contains the spores in wells along with the calcium ISEs. Ground experiments reveal a maximum calcium concentration ratio at 2.2xg, between micro-ion-selective electrodes near the “top” and “bottom” ends of the spore, indicating an increasing calcium concentration at one “end” of the fern spore with respect to the other. Confocal micrographs of rhizoid formation confirm the light-induced germination. SporeSat is a spaceflight experiment that will take ~ 4 days; data will be telemetered to Earth over ~ 100 days.
format	Conference or Workshop Item
author	Wan Salim, Wan Wardatul Amani
author_facet	Wan Salim, Wan Wardatul Amani
author_sort	Wan Salim, Wan Wardatul Amani
title	Electrochemical sensor centrifuge platform for single-cell study
title_short	Electrochemical sensor centrifuge platform for single-cell study
title_full	Electrochemical sensor centrifuge platform for single-cell study
title_fullStr	Electrochemical sensor centrifuge platform for single-cell study
title_full_unstemmed	Electrochemical sensor centrifuge platform for single-cell study
title_sort	electrochemical sensor centrifuge platform for single-cell study
publishDate	2015
url	http://irep.iium.edu.my/47224/ http://irep.iium.edu.my/47224/1/ACB2015_Abstract_Dr_Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/4/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/5/ACB_2015_Dr_Amani.pdf http://irep.iium.edu.my/47224/6/ACB_2015_presentation.pdf
first_indexed	2023-09-18T21:07:13Z
last_indexed	2023-09-18T21:07:13Z
_version_	1777411013369397248

Electrochemical sensor centrifuge platform for single-cell study

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