Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro

Background: Leukemia is one of the common cancers worldwide. Although the treatment options available for most type of cancers, but some of them remain incurable and the re-occurrence of disease is also common in many patients. Furthermore, modern therapy also leads to many side effects. All of thes...

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Main Authors:	Mamat, Suhana, Fadzir, Ummu Afifah, Zainudin, Muhammad Fauzan, Rosdi, Athirah, Abdul Wahab, Ridhwan, Hashim, Ridzwan
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/47178/ http://irep.iium.edu.my/47178/10/47178%20ANTILEUKEMOGENESIS%20OF%20NYPA%20FRUTICANS.pdf

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recordtype	eprints
spelling	iium-471782019-04-03T04:00:31Z http://irep.iium.edu.my/47178/ Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro Mamat, Suhana Fadzir, Ummu Afifah Zainudin, Muhammad Fauzan Rosdi, Athirah Abdul Wahab, Ridhwan Hashim, Ridzwan RB Pathology Background: Leukemia is one of the common cancers worldwide. Although the treatment options available for most type of cancers, but some of them remain incurable and the re-occurrence of disease is also common in many patients. Furthermore, modern therapy also leads to many side effects. All of these lead us to study on the possible anticancer activity of local fruit known as Nypa fruticans or locally known as nipah. Objective: This study was aimed to determine the antileukemogenesis effect of Nypa fruticans on leukemic cell lines (CCRF-CEM). Methodology: In this study, matured Nypa fruticans fruits was extracted using soxhlet apparatus. Methanol was used as the solvent for extraction. Different concentration of Nypa fruticans (3.125, 6.25, 12.5,25 & 50 µg/mL) extracts were tested on CCRF-CEM cells (lymphoblastic leukemia cell lines). Trypan blue assay was use to screen for concentration of nipah extracts that affect the viability of the cells after 24 hours of treatment. This was followed by determination of cells proliferation rate by BrdU assay. Results: The trypan blue assay showed prominent reduction in the cell viability when higher concentration of Nypa fruticans used to treat CCRF-CEM cell line. There were significant differences in concentration 12.5, 25 and 50 µg/mL compared to the control group. Furthermore, Brdu assay confirmed the decreasing trend in cell proliferation index following higher concentration of Nypa fruticans treatment on CCRF-CEM cells. Conclusion: This preliminary data suggest that, Nypa fruticans has the anti-leukemogenesis effect on acute lymphoblastic cell line. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/47178/10/47178%20ANTILEUKEMOGENESIS%20OF%20NYPA%20FRUTICANS.pdf Mamat, Suhana and Fadzir, Ummu Afifah and Zainudin, Muhammad Fauzan and Rosdi, Athirah and Abdul Wahab, Ridhwan and Hashim, Ridzwan (2015) Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro. In: 2nd USIM International Conference on Medicine and Health (ICMH 2015), 17th-18th October 2015, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Mamat, Suhana Fadzir, Ummu Afifah Zainudin, Muhammad Fauzan Rosdi, Athirah Abdul Wahab, Ridhwan Hashim, Ridzwan Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro
description	Background: Leukemia is one of the common cancers worldwide. Although the treatment options available for most type of cancers, but some of them remain incurable and the re-occurrence of disease is also common in many patients. Furthermore, modern therapy also leads to many side effects. All of these lead us to study on the possible anticancer activity of local fruit known as Nypa fruticans or locally known as nipah. Objective: This study was aimed to determine the antileukemogenesis effect of Nypa fruticans on leukemic cell lines (CCRF-CEM). Methodology: In this study, matured Nypa fruticans fruits was extracted using soxhlet apparatus. Methanol was used as the solvent for extraction. Different concentration of Nypa fruticans (3.125, 6.25, 12.5,25 & 50 µg/mL) extracts were tested on CCRF-CEM cells (lymphoblastic leukemia cell lines). Trypan blue assay was use to screen for concentration of nipah extracts that affect the viability of the cells after 24 hours of treatment. This was followed by determination of cells proliferation rate by BrdU assay. Results: The trypan blue assay showed prominent reduction in the cell viability when higher concentration of Nypa fruticans used to treat CCRF-CEM cell line. There were significant differences in concentration 12.5, 25 and 50 µg/mL compared to the control group. Furthermore, Brdu assay confirmed the decreasing trend in cell proliferation index following higher concentration of Nypa fruticans treatment on CCRF-CEM cells. Conclusion: This preliminary data suggest that, Nypa fruticans has the anti-leukemogenesis effect on acute lymphoblastic cell line.
format	Conference or Workshop Item
author	Mamat, Suhana Fadzir, Ummu Afifah Zainudin, Muhammad Fauzan Rosdi, Athirah Abdul Wahab, Ridhwan Hashim, Ridzwan
author_facet	Mamat, Suhana Fadzir, Ummu Afifah Zainudin, Muhammad Fauzan Rosdi, Athirah Abdul Wahab, Ridhwan Hashim, Ridzwan
author_sort	Mamat, Suhana
title	Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro
title_short	Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro
title_full	Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro
title_fullStr	Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro
title_full_unstemmed	Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro
title_sort	antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro
publishDate	2015
url	http://irep.iium.edu.my/47178/ http://irep.iium.edu.my/47178/10/47178%20ANTILEUKEMOGENESIS%20OF%20NYPA%20FRUTICANS.pdf
first_indexed	2023-09-18T21:07:09Z
last_indexed	2023-09-18T21:07:09Z
_version_	1777411009667923968

Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro

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