The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing

Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. This study investigates the correlation between cell attachment and impedance when cells are attached to two different substrates, polyaniline...

Full description

Bibliographic Details
Main Authors:	Ibrahim, Irmanisha, Nordin, Anis Nurashikin, Hashim, Yumi Zuhanis Has-Yun
Format:	Article
Language:	English English
Published:	Journal of Pure and Applied Microbiology 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/47157/ http://irep.iium.edu.my/47157/ http://irep.iium.edu.my/47157/1/Paper_-_Ibrahim_et_al_2014_%28JPAM%29_.pdf http://irep.iium.edu.my/47157/3/47157_The%20study%20of%20cell%20attachment%20and%20spreading_SCOPUS.pdf

id	iium-47157
recordtype	eprints
spelling	iium-471572018-01-09T08:02:40Z http://irep.iium.edu.my/47157/ The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing Ibrahim, Irmanisha Nordin, Anis Nurashikin Hashim, Yumi Zuhanis Has-Yun Q Science (General) Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. This study investigates the correlation between cell attachment and impedance when cells are attached to two different substrates, polyaniline and gelatin, respectively. Colon cancer cell lines, HCT-116 were used as model cell line. The impedance measurements were measured every 8 hours for 104 hours at frequency of 40Hz to 10MHz using impedance analyzer. Polyaniline produced a graph that is in agreement with typical growth curve for mammalian cell culture at lag, and early log phase. However, gelatin graph showed a different trend. This may be due to the high conductivity of the polyaniline which gave better cell attachment and spreading for the HCT-116 cells. The efficiency of the biosensor was measured by cytotoxicity test using 2.5 μg/ml 5-FU and the changes on impedance were analyzed. In conclusion, the cell attachment correlates with impedance depending on the substrate used to culture the cells. To this end, ECIS is proven as an alternative tool to measure cell behavior with an added advantage of ability to monitor the progress in real-time and showed a great potential in drug testing application. Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/47157/1/Paper_-_Ibrahim_et_al_2014_%28JPAM%29_.pdf application/pdf en http://irep.iium.edu.my/47157/3/47157_The%20study%20of%20cell%20attachment%20and%20spreading_SCOPUS.pdf Ibrahim, Irmanisha and Nordin, Anis Nurashikin and Hashim, Yumi Zuhanis Has-Yun (2014) The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing. Journal of Pure and Applied Microbiology, 8 (sp.ed). pp. 827-831. ISSN 0973-7510 http://www.microbiologyjournal.org/jmabsread.php?snoid=1888&month=&year=
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Ibrahim, Irmanisha Nordin, Anis Nurashikin Hashim, Yumi Zuhanis Has-Yun The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing
description	Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. This study investigates the correlation between cell attachment and impedance when cells are attached to two different substrates, polyaniline and gelatin, respectively. Colon cancer cell lines, HCT-116 were used as model cell line. The impedance measurements were measured every 8 hours for 104 hours at frequency of 40Hz to 10MHz using impedance analyzer. Polyaniline produced a graph that is in agreement with typical growth curve for mammalian cell culture at lag, and early log phase. However, gelatin graph showed a different trend. This may be due to the high conductivity of the polyaniline which gave better cell attachment and spreading for the HCT-116 cells. The efficiency of the biosensor was measured by cytotoxicity test using 2.5 μg/ml 5-FU and the changes on impedance were analyzed. In conclusion, the cell attachment correlates with impedance depending on the substrate used to culture the cells. To this end, ECIS is proven as an alternative tool to measure cell behavior with an added advantage of ability to monitor the progress in real-time and showed a great potential in drug testing application.
format	Article
author	Ibrahim, Irmanisha Nordin, Anis Nurashikin Hashim, Yumi Zuhanis Has-Yun
author_facet	Ibrahim, Irmanisha Nordin, Anis Nurashikin Hashim, Yumi Zuhanis Has-Yun
author_sort	Ibrahim, Irmanisha
title	The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing
title_short	The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing
title_full	The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing
title_fullStr	The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing
title_full_unstemmed	The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing
title_sort	study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing
publisher	Journal of Pure and Applied Microbiology
publishDate	2014
url	http://irep.iium.edu.my/47157/ http://irep.iium.edu.my/47157/ http://irep.iium.edu.my/47157/1/Paper_-_Ibrahim_et_al_2014_%28JPAM%29_.pdf http://irep.iium.edu.my/47157/3/47157_The%20study%20of%20cell%20attachment%20and%20spreading_SCOPUS.pdf
first_indexed	2023-09-18T21:07:07Z
last_indexed	2023-09-18T21:07:07Z
_version_	1777411007782584320

The study of cell attachment and spreading on polyaniline and gelatin using electric cell-substrate impedance sensing

Similar Items