Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods

Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods

Gelatin, derived from bovine and porcine sources, has been used in many foods and pharmaceutical products. To ensure the compliance of food products with halal regulations, the reliable analytical methods are very much required. In this study, polymerase chain reaction (PCR) assay using species-spec...

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Main Authors: Abdullah Amqizal, Hassan Ibrahem, Al-Kahtani, Hassan Abdullah M., Ismail, Elsayed Ali, Hayat, Khizar, Jaswir, Irwandi
Format: Article
Language:English
English
Published: Elsevier 2017
Subjects:
TP368 Food processing and manufacture
Online Access:http://irep.iium.edu.my/46727/
http://irep.iium.edu.my/46727/
http://irep.iium.edu.my/46727/
http://irep.iium.edu.my/46727/1/46727-Identification%20and%20verification%20of%20porcine%20DNA%20in%20commercial.pdf
http://irep.iium.edu.my/46727/2/46727-Identification%20and%20verification%20of%20porcine%20DNA%20in%20commercial_SCOPUS.pdf
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spelling iium-467272017-07-06T08:24:03Z http://irep.iium.edu.my/46727/ Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods Abdullah Amqizal, Hassan Ibrahem Al-Kahtani, Hassan Abdullah M. Ismail, Elsayed Ali Hayat, Khizar Jaswir, Irwandi TP368 Food processing and manufacture Gelatin, derived from bovine and porcine sources, has been used in many foods and pharmaceutical products. To ensure the compliance of food products with halal regulations, the reliable analytical methods are very much required. In this study, polymerase chain reaction (PCR) assay using species-specific primers was performed to evaluate the halal authenticity of commercial pure gelatin and gelatin-containing processed food products. Based on the specificity and cross-reactivity results of the seven species-specific primers by conventional PCR, the porcine species primer No. 2 was selected and it was able to detect species DNA in 12 out of 36 processed foods. The cloning, sequencing, and blasting at NCBI confirmed the presence of pork DNA in 5 out of 12 porcine DNA positive food samples. The maximum identity (homology) with pork sequence available in NCBI Gene Bank for the five samples ranged from 87% to 97% and the Query Cover ranged from 94% to 100%. The real-time PCR assay detected more positive samples (27 positive amplifications) compared to 12 positive samples with conventional PCR using porcine specific primer No. 2. PCR using species specific primers is a very useful and effective technique for halal authenticity of gelatin and gelatin-containing food products. Elsevier 2017-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46727/1/46727-Identification%20and%20verification%20of%20porcine%20DNA%20in%20commercial.pdf application/pdf en http://irep.iium.edu.my/46727/2/46727-Identification%20and%20verification%20of%20porcine%20DNA%20in%20commercial_SCOPUS.pdf Abdullah Amqizal, Hassan Ibrahem and Al-Kahtani, Hassan Abdullah M. and Ismail, Elsayed Ali and Hayat, Khizar and Jaswir, Irwandi (2017) Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods. Food Control, 78. pp. 297-303. E-ISSN 0956-7135 http://www.sciencedirect.com/science/article/pii/S0956713517300701 10.1016/j.foodcont.2017.02.024
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP368 Food processing and manufacture
spellingShingle TP368 Food processing and manufacture
Abdullah Amqizal, Hassan Ibrahem
Al-Kahtani, Hassan Abdullah M.
Ismail, Elsayed Ali
Hayat, Khizar
Jaswir, Irwandi
Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods
description Gelatin, derived from bovine and porcine sources, has been used in many foods and pharmaceutical products. To ensure the compliance of food products with halal regulations, the reliable analytical methods are very much required. In this study, polymerase chain reaction (PCR) assay using species-specific primers was performed to evaluate the halal authenticity of commercial pure gelatin and gelatin-containing processed food products. Based on the specificity and cross-reactivity results of the seven species-specific primers by conventional PCR, the porcine species primer No. 2 was selected and it was able to detect species DNA in 12 out of 36 processed foods. The cloning, sequencing, and blasting at NCBI confirmed the presence of pork DNA in 5 out of 12 porcine DNA positive food samples. The maximum identity (homology) with pork sequence available in NCBI Gene Bank for the five samples ranged from 87% to 97% and the Query Cover ranged from 94% to 100%. The real-time PCR assay detected more positive samples (27 positive amplifications) compared to 12 positive samples with conventional PCR using porcine specific primer No. 2. PCR using species specific primers is a very useful and effective technique for halal authenticity of gelatin and gelatin-containing food products.
format Article
author Abdullah Amqizal, Hassan Ibrahem
Al-Kahtani, Hassan Abdullah M.
Ismail, Elsayed Ali
Hayat, Khizar
Jaswir, Irwandi
author_facet Abdullah Amqizal, Hassan Ibrahem
Al-Kahtani, Hassan Abdullah M.
Ismail, Elsayed Ali
Hayat, Khizar
Jaswir, Irwandi
author_sort Abdullah Amqizal, Hassan Ibrahem
title Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods
title_short Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods
title_full Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods
title_fullStr Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods
title_full_unstemmed Identification and verification of porcine DNA in commercial gelatin and gelatin containing processed foods
title_sort identification and verification of porcine dna in commercial gelatin and gelatin containing processed foods
publisher Elsevier
publishDate 2017
url http://irep.iium.edu.my/46727/
http://irep.iium.edu.my/46727/
http://irep.iium.edu.my/46727/
http://irep.iium.edu.my/46727/1/46727-Identification%20and%20verification%20of%20porcine%20DNA%20in%20commercial.pdf
http://irep.iium.edu.my/46727/2/46727-Identification%20and%20verification%20of%20porcine%20DNA%20in%20commercial_SCOPUS.pdf
first_indexed 2023-09-18T21:06:31Z
last_indexed 2023-09-18T21:06:31Z
_version_ 1777410969382682624

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