Review on invitro amebcyte culture – a lesson learned from past

Limulus Amebocyte Lysate (LAL/TAL) assay is the only standard test approved by the Food and Drug Administration (FDA) to quantify bacterial endotoxin in injectable drugs and implantable medical devices. At present, horseshoe crabs are the sole source of LAL/TAL. Biomedical companies harvest and bl...

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Main Authors:	Hassan , Sheikh, John, Akbar, Arief Ichwan, Solachuddin Jauhari, Kassim, Zaleha, Yunus, Kamaruzzaman
Format:	Article
Language:	English
Published:	Universiti Teknologi Malaysia 2015
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/46532/ http://irep.iium.edu.my/46532/ http://irep.iium.edu.my/46532/1/56.pdf

id	iium-46532
recordtype	eprints
spelling	iium-465322016-05-19T00:26:32Z http://irep.iium.edu.my/46532/ Review on invitro amebcyte culture – a lesson learned from past Hassan , Sheikh John, Akbar Arief Ichwan, Solachuddin Jauhari Kassim, Zaleha Yunus, Kamaruzzaman Q Science (General) Limulus Amebocyte Lysate (LAL/TAL) assay is the only standard test approved by the Food and Drug Administration (FDA) to quantify bacterial endotoxin in injectable drugs and implantable medical devices. At present, horseshoe crabs are the sole source of LAL/TAL. Biomedical companies harvest and bleed horseshoe crabs which lead to ≤30% post bleeding mortality. The continuity of this practices will eventually deplete wild stock and threat horseshoe crab’s population. Though, many alternative biosensors were developed to quantify endotoxins, they are not without limitations and some of them even need LAL/TAL as source detectors. Hence, the LAL/TAL industry in its current form has ethical, ecological, commercial and technical issues that make it unideal standard test. An alternative method of culturing amebocyte in tissue culture medium has been addressed in literature since last 4 decades. This paper will address the issues in amebocyte culture in-vitro based on the published literatures. The paper will also suggest the best source of amebocyte cells (gill, gill flaps, blood), culture mode (monolayer or suspension), culture media (Grace's Insect Medium, Leibovitz’s L-15 Medium, Modified Essential medium, Shields and Sang insect medium, TNMFH Medium, RPMI 1640 medium) and best culture conditions (pH, temperature, osmolarity). This review will also emphasize on key elements in establishing a cell line for the continuous harvesting of amebocyte in-vitro. Universiti Teknologi Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46532/1/56.pdf Hassan , Sheikh and John, Akbar and Arief Ichwan, Solachuddin Jauhari and Kassim, Zaleha and Yunus, Kamaruzzaman (2015) Review on invitro amebcyte culture – a lesson learned from past. Jurnal Teknologi, 77 (25). pp. 171-176. ISSN 2180–3722 (O), 0127–9696 (P) 10.11113/jt.v77.6759
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Hassan , Sheikh John, Akbar Arief Ichwan, Solachuddin Jauhari Kassim, Zaleha Yunus, Kamaruzzaman Review on invitro amebcyte culture – a lesson learned from past
description	Limulus Amebocyte Lysate (LAL/TAL) assay is the only standard test approved by the Food and Drug Administration (FDA) to quantify bacterial endotoxin in injectable drugs and implantable medical devices. At present, horseshoe crabs are the sole source of LAL/TAL. Biomedical companies harvest and bleed horseshoe crabs which lead to ≤30% post bleeding mortality. The continuity of this practices will eventually deplete wild stock and threat horseshoe crab’s population. Though, many alternative biosensors were developed to quantify endotoxins, they are not without limitations and some of them even need LAL/TAL as source detectors. Hence, the LAL/TAL industry in its current form has ethical, ecological, commercial and technical issues that make it unideal standard test. An alternative method of culturing amebocyte in tissue culture medium has been addressed in literature since last 4 decades. This paper will address the issues in amebocyte culture in-vitro based on the published literatures. The paper will also suggest the best source of amebocyte cells (gill, gill flaps, blood), culture mode (monolayer or suspension), culture media (Grace's Insect Medium, Leibovitz’s L-15 Medium, Modified Essential medium, Shields and Sang insect medium, TNMFH Medium, RPMI 1640 medium) and best culture conditions (pH, temperature, osmolarity). This review will also emphasize on key elements in establishing a cell line for the continuous harvesting of amebocyte in-vitro.
format	Article
author	Hassan , Sheikh John, Akbar Arief Ichwan, Solachuddin Jauhari Kassim, Zaleha Yunus, Kamaruzzaman
author_facet	Hassan , Sheikh John, Akbar Arief Ichwan, Solachuddin Jauhari Kassim, Zaleha Yunus, Kamaruzzaman
author_sort	Hassan , Sheikh
title	Review on invitro amebcyte culture – a lesson learned from past
title_short	Review on invitro amebcyte culture – a lesson learned from past
title_full	Review on invitro amebcyte culture – a lesson learned from past
title_fullStr	Review on invitro amebcyte culture – a lesson learned from past
title_full_unstemmed	Review on invitro amebcyte culture – a lesson learned from past
title_sort	review on invitro amebcyte culture – a lesson learned from past
publisher	Universiti Teknologi Malaysia
publishDate	2015
url	http://irep.iium.edu.my/46532/ http://irep.iium.edu.my/46532/ http://irep.iium.edu.my/46532/1/56.pdf
first_indexed	2023-09-18T21:06:15Z
last_indexed	2023-09-18T21:06:15Z
_version_	1777410952691449856

Review on invitro amebcyte culture – a lesson learned from past

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