Review on invitro amebcyte culture – a lesson learned from past

Limulus Amebocyte Lysate (LAL/TAL) assay is the only standard test approved by the Food and Drug Administration (FDA) to quantify bacterial endotoxin in injectable drugs and implantable medical devices. At present, horseshoe crabs are the sole source of LAL/TAL. Biomedical companies harvest and bl...

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Main Authors: Hassan , Sheikh, John, Akbar, Arief Ichwan, Solachuddin Jauhari, Kassim, Zaleha, Yunus, Kamaruzzaman
Format: Article
Language:English
Published: Universiti Teknologi Malaysia 2015
Subjects:
Online Access:http://irep.iium.edu.my/46532/
http://irep.iium.edu.my/46532/
http://irep.iium.edu.my/46532/1/56.pdf
id iium-46532
recordtype eprints
spelling iium-465322016-05-19T00:26:32Z http://irep.iium.edu.my/46532/ Review on invitro amebcyte culture – a lesson learned from past Hassan , Sheikh John, Akbar Arief Ichwan, Solachuddin Jauhari Kassim, Zaleha Yunus, Kamaruzzaman Q Science (General) Limulus Amebocyte Lysate (LAL/TAL) assay is the only standard test approved by the Food and Drug Administration (FDA) to quantify bacterial endotoxin in injectable drugs and implantable medical devices. At present, horseshoe crabs are the sole source of LAL/TAL. Biomedical companies harvest and bleed horseshoe crabs which lead to ≤30% post bleeding mortality. The continuity of this practices will eventually deplete wild stock and threat horseshoe crab’s population. Though, many alternative biosensors were developed to quantify endotoxins, they are not without limitations and some of them even need LAL/TAL as source detectors. Hence, the LAL/TAL industry in its current form has ethical, ecological, commercial and technical issues that make it unideal standard test. An alternative method of culturing amebocyte in tissue culture medium has been addressed in literature since last 4 decades. This paper will address the issues in amebocyte culture in-vitro based on the published literatures. The paper will also suggest the best source of amebocyte cells (gill, gill flaps, blood), culture mode (monolayer or suspension), culture media (Grace's Insect Medium, Leibovitz’s L-15 Medium, Modified Essential medium, Shields and Sang insect medium, TNMFH Medium, RPMI 1640 medium) and best culture conditions (pH, temperature, osmolarity). This review will also emphasize on key elements in establishing a cell line for the continuous harvesting of amebocyte in-vitro. Universiti Teknologi Malaysia 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46532/1/56.pdf Hassan , Sheikh and John, Akbar and Arief Ichwan, Solachuddin Jauhari and Kassim, Zaleha and Yunus, Kamaruzzaman (2015) Review on invitro amebcyte culture – a lesson learned from past. Jurnal Teknologi, 77 (25). pp. 171-176. ISSN 2180–3722 (O), 0127–9696 (P) 10.11113/jt.v77.6759
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic Q Science (General)
spellingShingle Q Science (General)
Hassan , Sheikh
John, Akbar
Arief Ichwan, Solachuddin Jauhari
Kassim, Zaleha
Yunus, Kamaruzzaman
Review on invitro amebcyte culture – a lesson learned from past
description Limulus Amebocyte Lysate (LAL/TAL) assay is the only standard test approved by the Food and Drug Administration (FDA) to quantify bacterial endotoxin in injectable drugs and implantable medical devices. At present, horseshoe crabs are the sole source of LAL/TAL. Biomedical companies harvest and bleed horseshoe crabs which lead to ≤30% post bleeding mortality. The continuity of this practices will eventually deplete wild stock and threat horseshoe crab’s population. Though, many alternative biosensors were developed to quantify endotoxins, they are not without limitations and some of them even need LAL/TAL as source detectors. Hence, the LAL/TAL industry in its current form has ethical, ecological, commercial and technical issues that make it unideal standard test. An alternative method of culturing amebocyte in tissue culture medium has been addressed in literature since last 4 decades. This paper will address the issues in amebocyte culture in-vitro based on the published literatures. The paper will also suggest the best source of amebocyte cells (gill, gill flaps, blood), culture mode (monolayer or suspension), culture media (Grace's Insect Medium, Leibovitz’s L-15 Medium, Modified Essential medium, Shields and Sang insect medium, TNMFH Medium, RPMI 1640 medium) and best culture conditions (pH, temperature, osmolarity). This review will also emphasize on key elements in establishing a cell line for the continuous harvesting of amebocyte in-vitro.
format Article
author Hassan , Sheikh
John, Akbar
Arief Ichwan, Solachuddin Jauhari
Kassim, Zaleha
Yunus, Kamaruzzaman
author_facet Hassan , Sheikh
John, Akbar
Arief Ichwan, Solachuddin Jauhari
Kassim, Zaleha
Yunus, Kamaruzzaman
author_sort Hassan , Sheikh
title Review on invitro amebcyte culture – a lesson learned from past
title_short Review on invitro amebcyte culture – a lesson learned from past
title_full Review on invitro amebcyte culture – a lesson learned from past
title_fullStr Review on invitro amebcyte culture – a lesson learned from past
title_full_unstemmed Review on invitro amebcyte culture – a lesson learned from past
title_sort review on invitro amebcyte culture – a lesson learned from past
publisher Universiti Teknologi Malaysia
publishDate 2015
url http://irep.iium.edu.my/46532/
http://irep.iium.edu.my/46532/
http://irep.iium.edu.my/46532/1/56.pdf
first_indexed 2023-09-18T21:06:15Z
last_indexed 2023-09-18T21:06:15Z
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