Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy

Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological prope...

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Main Authors: Doolaanea, Abd Almonem, Mansor, Nur 'Izzati, Mohd Nor, Nurul Hafizah, Mohamed, Farahidah
Format: Conference or Workshop Item
Language:English
English
Published: UKM Kuala Lumpur Campus 2015
Subjects:
Online Access:http://irep.iium.edu.my/46387/
http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf
http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf
id iium-46387
recordtype eprints
spelling iium-463872016-05-27T02:43:09Z http://irep.iium.edu.my/46387/ Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah RS Pharmacy and materia medica Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological properties. CTAB was found to adsorb on the surface of F1 with easy desorption. This led to negative zeta potential, high cytotoxicity and low cell uptake/transfection. F2 exhibited negative zeta potential, low cytotoxicity, and higher cell uptake/transfection. F3 and F4 showed positive zeta potential, low cytotoxicity and high cell uptake/transfection. Due to its stronger interaction with PLGA that may stabilise the plasmid DNA, F4 exhibited higher transfection efficacy. This study highlights the significant differences between CTAB and chitosan in modifying nanoparticles for gene therapy. UKM Kuala Lumpur Campus 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf application/pdf en http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2015) Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy. In: Controlled Released & Delivery Symposium 2015 (CRDDS2015), 15th-16th Aug. 2015, Faculty of Pharmacy, Universiti Kebangsaan Malaysia. (Unpublished)
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Doolaanea, Abd Almonem
Mansor, Nur 'Izzati
Mohd Nor, Nurul Hafizah
Mohamed, Farahidah
Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
description Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological properties. CTAB was found to adsorb on the surface of F1 with easy desorption. This led to negative zeta potential, high cytotoxicity and low cell uptake/transfection. F2 exhibited negative zeta potential, low cytotoxicity, and higher cell uptake/transfection. F3 and F4 showed positive zeta potential, low cytotoxicity and high cell uptake/transfection. Due to its stronger interaction with PLGA that may stabilise the plasmid DNA, F4 exhibited higher transfection efficacy. This study highlights the significant differences between CTAB and chitosan in modifying nanoparticles for gene therapy.
format Conference or Workshop Item
author Doolaanea, Abd Almonem
Mansor, Nur 'Izzati
Mohd Nor, Nurul Hafizah
Mohamed, Farahidah
author_facet Doolaanea, Abd Almonem
Mansor, Nur 'Izzati
Mohd Nor, Nurul Hafizah
Mohamed, Farahidah
author_sort Doolaanea, Abd Almonem
title Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_short Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_full Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_fullStr Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_full_unstemmed Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_sort differences between ctab and chitosan in modifying plga nanoparticles for gene therapy
publisher UKM Kuala Lumpur Campus
publishDate 2015
url http://irep.iium.edu.my/46387/
http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf
http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf
first_indexed 2023-09-18T21:06:02Z
last_indexed 2023-09-18T21:06:02Z
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