Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy

Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological prope...

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Main Authors:	Doolaanea, Abd Almonem, Mansor, Nur 'Izzati, Mohd Nor, Nurul Hafizah, Mohamed, Farahidah
Format:	Conference or Workshop Item
Language:	English English
Published:	UKM Kuala Lumpur Campus 2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/46387/ http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf

id	iium-46387
recordtype	eprints
spelling	iium-463872016-05-27T02:43:09Z http://irep.iium.edu.my/46387/ Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah RS Pharmacy and materia medica Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological properties. CTAB was found to adsorb on the surface of F1 with easy desorption. This led to negative zeta potential, high cytotoxicity and low cell uptake/transfection. F2 exhibited negative zeta potential, low cytotoxicity, and higher cell uptake/transfection. F3 and F4 showed positive zeta potential, low cytotoxicity and high cell uptake/transfection. Due to its stronger interaction with PLGA that may stabilise the plasmid DNA, F4 exhibited higher transfection efficacy. This study highlights the significant differences between CTAB and chitosan in modifying nanoparticles for gene therapy. UKM Kuala Lumpur Campus 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf application/pdf en http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2015) Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy. In: Controlled Released & Delivery Symposium 2015 (CRDDS2015), 15th-16th Aug. 2015, Faculty of Pharmacy, Universiti Kebangsaan Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
description	Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological properties. CTAB was found to adsorb on the surface of F1 with easy desorption. This led to negative zeta potential, high cytotoxicity and low cell uptake/transfection. F2 exhibited negative zeta potential, low cytotoxicity, and higher cell uptake/transfection. F3 and F4 showed positive zeta potential, low cytotoxicity and high cell uptake/transfection. Due to its stronger interaction with PLGA that may stabilise the plasmid DNA, F4 exhibited higher transfection efficacy. This study highlights the significant differences between CTAB and chitosan in modifying nanoparticles for gene therapy.
format	Conference or Workshop Item
author	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_short	Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_full	Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_fullStr	Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_full_unstemmed	Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
title_sort	differences between ctab and chitosan in modifying plga nanoparticles for gene therapy
publisher	UKM Kuala Lumpur Campus
publishDate	2015
url	http://irep.iium.edu.my/46387/ http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf
first_indexed	2023-09-18T21:06:02Z
last_indexed	2023-09-18T21:06:02Z
_version_	1777410939138605056

Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy

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