Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production

Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production

Effective bioconversion of lignocellulosic biomass into soluble sugars for bioethanol production requires use of thermostable and highly active cellulases. Lignocellulose is the most abundant biopolymer on earth and it is a good renewable energy source. Thermostable enzymes have several benefits in...

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Bibliographic Details
Main Authors: Ibrahim Ali , Noorbatcha, Hamzah, Mohd. Salleh
Format: Conference or Workshop Item
Language:English
English
English
English
Published: 2015
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/46336/
http://irep.iium.edu.my/46336/1/Poster_ACB2015_Ibrahim.pdf
http://irep.iium.edu.my/46336/4/Prog_book_BPE06_ACB2015_.pdf
http://irep.iium.edu.my/46336/5/BPE06_Abstract_ACB2015.pdf
http://irep.iium.edu.my/46336/8/ACB2015_prog_book_cover_page.pdf
id iium-46336
recordtype eprints
spelling iium-463362016-05-24T00:06:38Z http://irep.iium.edu.my/46336/ Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production Ibrahim Ali , Noorbatcha Hamzah, Mohd. Salleh TP248.13 Biotechnology Effective bioconversion of lignocellulosic biomass into soluble sugars for bioethanol production requires use of thermostable and highly active cellulases. Lignocellulose is the most abundant biopolymer on earth and it is a good renewable energy source. Thermostable enzymes have several benefits in various industrial applications. This research intends to predict computationally, and develop experimentally, a thermostable mutant endoglucanase that can operate at higher temperature as required by industrial processes. Our preliminary computational data based on rational design approach suggest that a quintuple mutant (T224E/G229A/S230F/S231E/N321R) of an endoglucanase from a pathogenic fungus, Fusarium oxysporum, can potentially remain active at higher temperature (80oC) compared to its current operating temperature (<60oC). In our effort to experimentally produce this quintuple mutant we have successfully produced few mutants via site directed mutagenesis. We are reporting here the characterization results of these mutants as compared to the wild type. Keywords: Thermostable enzymes, mutant endoglucanases, Fusarium oxysporum 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/46336/1/Poster_ACB2015_Ibrahim.pdf application/pdf en http://irep.iium.edu.my/46336/4/Prog_book_BPE06_ACB2015_.pdf application/pdf en http://irep.iium.edu.my/46336/5/BPE06_Abstract_ACB2015.pdf application/pdf en http://irep.iium.edu.my/46336/8/ACB2015_prog_book_cover_page.pdf Ibrahim Ali , Noorbatcha and Hamzah, Mohd. Salleh (2015) Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production. In: ASIAN Congress on Biotechnology 2015, 15th-19th Nov. 2015, Kuala Lumpur.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Ibrahim Ali , Noorbatcha
Hamzah, Mohd. Salleh
Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production
description Effective bioconversion of lignocellulosic biomass into soluble sugars for bioethanol production requires use of thermostable and highly active cellulases. Lignocellulose is the most abundant biopolymer on earth and it is a good renewable energy source. Thermostable enzymes have several benefits in various industrial applications. This research intends to predict computationally, and develop experimentally, a thermostable mutant endoglucanase that can operate at higher temperature as required by industrial processes. Our preliminary computational data based on rational design approach suggest that a quintuple mutant (T224E/G229A/S230F/S231E/N321R) of an endoglucanase from a pathogenic fungus, Fusarium oxysporum, can potentially remain active at higher temperature (80oC) compared to its current operating temperature (<60oC). In our effort to experimentally produce this quintuple mutant we have successfully produced few mutants via site directed mutagenesis. We are reporting here the characterization results of these mutants as compared to the wild type. Keywords: Thermostable enzymes, mutant endoglucanases, Fusarium oxysporum
format Conference or Workshop Item
author Ibrahim Ali , Noorbatcha
Hamzah, Mohd. Salleh
author_facet Ibrahim Ali , Noorbatcha
Hamzah, Mohd. Salleh
author_sort Ibrahim Ali , Noorbatcha
title Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production
title_short Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production
title_full Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production
title_fullStr Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production
title_full_unstemmed Characterization of a potential thermostable endoglucanase I from fusarium oxysporum & possible applications in bioethanol production
title_sort characterization of a potential thermostable endoglucanase i from fusarium oxysporum & possible applications in bioethanol production
publishDate 2015
url http://irep.iium.edu.my/46336/
http://irep.iium.edu.my/46336/1/Poster_ACB2015_Ibrahim.pdf
http://irep.iium.edu.my/46336/4/Prog_book_BPE06_ACB2015_.pdf
http://irep.iium.edu.my/46336/5/BPE06_Abstract_ACB2015.pdf
http://irep.iium.edu.my/46336/8/ACB2015_prog_book_cover_page.pdf
first_indexed 2023-09-18T21:05:58Z
last_indexed 2023-09-18T21:05:58Z
_version_ 1777410935029235712

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