Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres

Purpose: To evaluate the effect of surfactants on plasmid DNA during preparation and release from polylactic glycolide (PLGA) microspheres. Methods: Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100, cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added dur...

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Main Authors:	Doolaanea, Abd Almonem, Ismail, Ahmad Fahmi Harun, Mansor, Nur‘Izzati, Nor, Nurul Hafizah Mohd, Mohamed, Farahidah
Format:	Article
Language:	English
Published:	Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2015
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/1/Effect_of_Surfactants_on_Plasmid_DNA_Stability_and_Release.pdf

id	iium-46259
recordtype	eprints
spelling	iium-462592016-05-15T19:09:26Z http://irep.iium.edu.my/46259/ Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres Doolaanea, Abd Almonem Ismail, Ahmad Fahmi Harun Mansor, Nur‘Izzati Nor, Nurul Hafizah Mohd Mohamed, Farahidah RS Pharmacy and materia medica Purpose: To evaluate the effect of surfactants on plasmid DNA during preparation and release from polylactic glycolide (PLGA) microspheres. Methods: Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100, cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added during the microsphere preparation and their effect was evaluated. Supercoil index (SCI) was introduced as a harmonised value derived from encapsulation efficiency and supercoil preservation efficiency in order to evaluate the impact of different surfactants on pDNA encapsulation. Results: Polyvinyl alcohol and Span revealed low SCI whereas Tween increased the SCI in a fraction- dependent manner. The Tween blend of hydrophilic-lipophilic balance (HLB) of 16 and Triton X-100 (HLB = 13.5) showed the highest SCI. Span revealed high burst release of pDNA whereas Triton X-100 exhibited low burst release. Following the burst release, diffusion mechanism was found to predominate in DNA release. Conclusion: The microspheres were non-toxic to the neuro-2a cells which suggest they can be potentially used in the gene therapy of neuronal diseases. Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2015-10 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/46259/1/Effect_of_Surfactants_on_Plasmid_DNA_Stability_and_Release.pdf Doolaanea, Abd Almonem and Ismail, Ahmad Fahmi Harun and Mansor, Nur‘Izzati and Nor, Nurul Hafizah Mohd and Mohamed, Farahidah (2015) Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres. Tropical Journal of Pharmaceutical Research, 14 (10). pp. 1769-1778. ISSN 1596-5996 E-ISSN 1596-9827 http://www.ajol.info/index.php/tjpr/article/view/125241 http://dx.doi.org/10.4314/tjpr.v14i10.6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Doolaanea, Abd Almonem Ismail, Ahmad Fahmi Harun Mansor, Nur‘Izzati Nor, Nurul Hafizah Mohd Mohamed, Farahidah Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres
description	Purpose: To evaluate the effect of surfactants on plasmid DNA during preparation and release from polylactic glycolide (PLGA) microspheres. Methods: Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100, cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added during the microsphere preparation and their effect was evaluated. Supercoil index (SCI) was introduced as a harmonised value derived from encapsulation efficiency and supercoil preservation efficiency in order to evaluate the impact of different surfactants on pDNA encapsulation. Results: Polyvinyl alcohol and Span revealed low SCI whereas Tween increased the SCI in a fraction- dependent manner. The Tween blend of hydrophilic-lipophilic balance (HLB) of 16 and Triton X-100 (HLB = 13.5) showed the highest SCI. Span revealed high burst release of pDNA whereas Triton X-100 exhibited low burst release. Following the burst release, diffusion mechanism was found to predominate in DNA release. Conclusion: The microspheres were non-toxic to the neuro-2a cells which suggest they can be potentially used in the gene therapy of neuronal diseases.
format	Article
author	Doolaanea, Abd Almonem Ismail, Ahmad Fahmi Harun Mansor, Nur‘Izzati Nor, Nurul Hafizah Mohd Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Ismail, Ahmad Fahmi Harun Mansor, Nur‘Izzati Nor, Nurul Hafizah Mohd Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres
title_short	Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres
title_full	Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres
title_fullStr	Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres
title_full_unstemmed	Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres
title_sort	effect of surfactants on plasmid dna stability and release from poly (d, l-lactide-co-glycolide) microspheres
publisher	Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
publishDate	2015
url	http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/1/Effect_of_Surfactants_on_Plasmid_DNA_Stability_and_Release.pdf
first_indexed	2023-09-18T21:05:52Z
last_indexed	2023-09-18T21:05:52Z
_version_	1777410928351903744

Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres

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