Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres
Purpose: To evaluate the effect of surfactants on plasmid DNA during preparation and release from polylactic glycolide (PLGA) microspheres. Methods: Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100, cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added dur...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/ http://irep.iium.edu.my/46259/1/Effect_of_Surfactants_on_Plasmid_DNA_Stability_and_Release.pdf |
| Summary: | Purpose: To evaluate the effect of surfactants on plasmid DNA during preparation and release from
polylactic glycolide (PLGA) microspheres.
Methods: Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100,
cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added during the microsphere
preparation and their effect was evaluated. Supercoil index (SCI) was introduced as a harmonised value
derived from encapsulation efficiency and supercoil preservation efficiency in order to evaluate the
impact of different surfactants on pDNA encapsulation.
Results: Polyvinyl alcohol and Span revealed low SCI whereas Tween increased the SCI in a fraction-
dependent manner. The Tween blend of hydrophilic-lipophilic balance (HLB) of 16 and Triton X-100
(HLB = 13.5) showed the highest SCI. Span revealed high burst release of pDNA whereas Triton X-100
exhibited low burst release. Following the burst release, diffusion mechanism was found to predominate
in DNA release.
Conclusion: The microspheres were non-toxic to the neuro-2a cells which suggest they can be
potentially used in the gene therapy of neuronal diseases. |
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