Relationship between metabolites composition and biological activities of phyllanthus niruri extracts prepared by different drying methods and solvents extraction
The study investigated the changes in the metabolite, antioxidant and α-glucosidase inhibitory activities of Phyllanthus niruri after three drying treatments: air, freeze and oven dryings. Water extracts and extracts obtained using different solvent ratios of ethanol and methanol (50, 70, 80 an...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/46214/ http://irep.iium.edu.my/46214/ http://irep.iium.edu.my/46214/ http://irep.iium.edu.my/46214/1/mediani_et_al_2015.pdf |
| Summary: | The study investigated the changes in the metabolite,
antioxidant and α-glucosidase inhibitory activities of
Phyllanthus niruri after three drying treatments: air, freeze
and oven dryings. Water extracts and extracts obtained
using different solvent ratios of ethanol and methanol (50,
70, 80 and 100 %) were compared. The relationships
among the antioxidant, α-glucosidase inhibitory activity
and metabolite levels of the extracts were evaluated using
partial least-square analysis (PLS). The solvent selectivity
was assessed based on the phytochemical constituents present
in the extract and their concentrations quantitatively
analyzed using high performance liquid chromatography.
The freeze-dried P. niruri samples that were extracted with
the mixture of ethanol or methanol with low ratio of water
showed higher biological activity values compared with the other extracts. The PLS results for the ethanolic with different
ratio and water extracts demonstrated that phenolic
acids (chlorogenic acid and ellagic acid) and flavonoids
were highly linked to strong α-glucosidase inhibitory and
antioxidant activities. |
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