Antibacterial activity from Malaysian mango kernel

Mango (Mangifera indica L.) is a fruit belonging to the genus Mangifera and family Anacardiaceae, consisting of numerous species of tropical fruiting trees in the flowering plant. Mango has been reported to have high antibacterial activity against gram positive bacteria, aids the development of the...

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Main Authors:	Al-Shwyeh, Hussah Abdullah, Mirghani, Mohamed Elwathig Saeed, Jamal, Parveen
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/4615/ http://irep.iium.edu.my/4615/ http://irep.iium.edu.my/4615/1/Full_Paper_for_ICBIOE2011_Hussah.pdf

id	iium-4615
recordtype	eprints
spelling	iium-46152011-10-05T06:40:00Z http://irep.iium.edu.my/4615/ Antibacterial activity from Malaysian mango kernel Al-Shwyeh, Hussah Abdullah Mirghani, Mohamed Elwathig Saeed Jamal, Parveen QR Microbiology Mango (Mangifera indica L.) is a fruit belonging to the genus Mangifera and family Anacardiaceae, consisting of numerous species of tropical fruiting trees in the flowering plant. Mango has been reported to have high antibacterial activity against gram positive bacteria, aids the development of the placenta and fetus, and helps the metabolic activities of teeth, the retina and skin, while preventing anemia. In addition, it also helps to tighten the capillary vessels. However, the significant increases of mango consumption in domestic activity lead to the accumulation of waste especially its kernel. This study attempts to screen three varieties of Mango kernels; waterlily, lemak and shakran extracted using four different extraction solvent; ethanol, methanol, acetone and distilled water to examine the potential of Mango kernel as natural antibacterial against four bacterial strains; Staphylococcus aureus, Bacillus subtillis, Escherichia coli and Pseudomonas aeruginosa. Disc diffusion assay was employed to determine the antibacterial activity. Later, the optimization of process conditions for extraction of antibacterial activity (having widest inhibition zone) was conducted in shake flasks based on the experimental design suggested by Central Composite Design (CCD) under Response Surface Methodology (RSM) from Design Expert v.6.0.8. by manipulating reaction temperature (ºC), reaction time (hour) and agitation speed (rpm). It was found out that waterlily has the best antibacterial activity utilizing ethanol as the extraction solvent. Optimization of this sample was run and the maximum antibacterial activity (16.80 mm) was reached at 37ºC, 24 hours and 200 rpm. Identification of the active compound using GC-MS recognized phenol, 2, 4-bis (1, 1-dimethylethyl) as the possible compound accountable for antibacterial activity. This finding would probably become an alternative source of new and natural antibacterial agents. 2011-05-17 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/4615/1/Full_Paper_for_ICBIOE2011_Hussah.pdf Al-Shwyeh, Hussah Abdullah and Mirghani, Mohamed Elwathig Saeed and Jamal, Parveen (2011) Antibacterial activity from Malaysian mango kernel. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. Http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Al-Shwyeh, Hussah Abdullah Mirghani, Mohamed Elwathig Saeed Jamal, Parveen Antibacterial activity from Malaysian mango kernel
description	Mango (Mangifera indica L.) is a fruit belonging to the genus Mangifera and family Anacardiaceae, consisting of numerous species of tropical fruiting trees in the flowering plant. Mango has been reported to have high antibacterial activity against gram positive bacteria, aids the development of the placenta and fetus, and helps the metabolic activities of teeth, the retina and skin, while preventing anemia. In addition, it also helps to tighten the capillary vessels. However, the significant increases of mango consumption in domestic activity lead to the accumulation of waste especially its kernel. This study attempts to screen three varieties of Mango kernels; waterlily, lemak and shakran extracted using four different extraction solvent; ethanol, methanol, acetone and distilled water to examine the potential of Mango kernel as natural antibacterial against four bacterial strains; Staphylococcus aureus, Bacillus subtillis, Escherichia coli and Pseudomonas aeruginosa. Disc diffusion assay was employed to determine the antibacterial activity. Later, the optimization of process conditions for extraction of antibacterial activity (having widest inhibition zone) was conducted in shake flasks based on the experimental design suggested by Central Composite Design (CCD) under Response Surface Methodology (RSM) from Design Expert v.6.0.8. by manipulating reaction temperature (ºC), reaction time (hour) and agitation speed (rpm). It was found out that waterlily has the best antibacterial activity utilizing ethanol as the extraction solvent. Optimization of this sample was run and the maximum antibacterial activity (16.80 mm) was reached at 37ºC, 24 hours and 200 rpm. Identification of the active compound using GC-MS recognized phenol, 2, 4-bis (1, 1-dimethylethyl) as the possible compound accountable for antibacterial activity. This finding would probably become an alternative source of new and natural antibacterial agents.
format	Conference or Workshop Item
author	Al-Shwyeh, Hussah Abdullah Mirghani, Mohamed Elwathig Saeed Jamal, Parveen
author_facet	Al-Shwyeh, Hussah Abdullah Mirghani, Mohamed Elwathig Saeed Jamal, Parveen
author_sort	Al-Shwyeh, Hussah Abdullah
title	Antibacterial activity from Malaysian mango kernel
title_short	Antibacterial activity from Malaysian mango kernel
title_full	Antibacterial activity from Malaysian mango kernel
title_fullStr	Antibacterial activity from Malaysian mango kernel
title_full_unstemmed	Antibacterial activity from Malaysian mango kernel
title_sort	antibacterial activity from malaysian mango kernel
publishDate	2011
url	http://irep.iium.edu.my/4615/ http://irep.iium.edu.my/4615/ http://irep.iium.edu.my/4615/1/Full_Paper_for_ICBIOE2011_Hussah.pdf
first_indexed	2023-09-18T20:12:54Z
last_indexed	2023-09-18T20:12:54Z
_version_	1777407596205965312

Antibacterial activity from Malaysian mango kernel

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