Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis

ARID3A and ARID3B are transcriptional targets of p53. Recently, it has been reported that ARID3A plays a critical role in the transcriptional activation of pro-arrest p21 in response to DNA damage. However, the role of ARID3B in the p53 regulatory pathway remains poorly understood. Here we show th...

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Main Authors:	Pratama, Endrawan, Tian, Xiaohui, Lestari, Widya, Iseki, Sachiko, Arief Ichwan, Solachuddin Jauhari, Ikeda, Masa-Aki
Format:	Article
Language:	English
Published:	Elsevier 2015
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/45991/ http://irep.iium.edu.my/45991/ http://irep.iium.edu.my/45991/ http://irep.iium.edu.my/45991/1/Endrawan_BBRC_at_PUBMED.pdf

id	iium-45991
recordtype	eprints
spelling	iium-459912016-05-12T03:38:58Z http://irep.iium.edu.my/45991/ Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis Pratama, Endrawan Tian, Xiaohui Lestari, Widya Iseki, Sachiko Arief Ichwan, Solachuddin Jauhari Ikeda, Masa-Aki RK Dentistry ARID3A and ARID3B are transcriptional targets of p53. Recently, it has been reported that ARID3A plays a critical role in the transcriptional activation of pro-arrest p21 in response to DNA damage. However, the role of ARID3B in the p53 regulatory pathway remains poorly understood. Here we show that ARID3A and ARID3B specifically bind to putative ARID3-binding sites in p53 target genes in vitro and in vivo. ARID3B and, to a lesser extent, ARID3A silencing blocked transcriptional activation of pro-apoptotic p53 target genes, such as PUMA, PIG3, and p53. Furthermore, ectopic ARID3B, to a lesser extent, ARID3A expression activated the pro-apoptotic gene expression, and only ARID3B induced apoptosis. Finally, ARID3B but not ARID3A silencing blocked apoptosis induction following DNA damage. These results indicated that, although ARID3B and ARID3A share overlapping functions, ARID3B play a key role in the expression of pro-apoptotic p53-target genes and apoptosis. Elsevier 2015-12-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/45991/1/Endrawan_BBRC_at_PUBMED.pdf Pratama, Endrawan and Tian, Xiaohui and Lestari, Widya and Iseki, Sachiko and Arief Ichwan, Solachuddin Jauhari and Ikeda, Masa-Aki (2015) Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis. Biochemical and Biophysical Research Communications, 468 (1-2). pp. 248-254. ISSN 0006-291X http://www.sciencedirect.com/science/article/pii/S0006291X15308202 10.1016/j.bbrc.2015.10.121
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Pratama, Endrawan Tian, Xiaohui Lestari, Widya Iseki, Sachiko Arief Ichwan, Solachuddin Jauhari Ikeda, Masa-Aki Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis
description	ARID3A and ARID3B are transcriptional targets of p53. Recently, it has been reported that ARID3A plays a critical role in the transcriptional activation of pro-arrest p21 in response to DNA damage. However, the role of ARID3B in the p53 regulatory pathway remains poorly understood. Here we show that ARID3A and ARID3B specifically bind to putative ARID3-binding sites in p53 target genes in vitro and in vivo. ARID3B and, to a lesser extent, ARID3A silencing blocked transcriptional activation of pro-apoptotic p53 target genes, such as PUMA, PIG3, and p53. Furthermore, ectopic ARID3B, to a lesser extent, ARID3A expression activated the pro-apoptotic gene expression, and only ARID3B induced apoptosis. Finally, ARID3B but not ARID3A silencing blocked apoptosis induction following DNA damage. These results indicated that, although ARID3B and ARID3A share overlapping functions, ARID3B play a key role in the expression of pro-apoptotic p53-target genes and apoptosis.
format	Article
author	Pratama, Endrawan Tian, Xiaohui Lestari, Widya Iseki, Sachiko Arief Ichwan, Solachuddin Jauhari Ikeda, Masa-Aki
author_facet	Pratama, Endrawan Tian, Xiaohui Lestari, Widya Iseki, Sachiko Arief Ichwan, Solachuddin Jauhari Ikeda, Masa-Aki
author_sort	Pratama, Endrawan
title	Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis
title_short	Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis
title_full	Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis
title_fullStr	Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis
title_full_unstemmed	Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis
title_sort	critical role of arid3b in the expression of pro-apoptotic p53-target genes and apoptosis
publisher	Elsevier
publishDate	2015
url	http://irep.iium.edu.my/45991/ http://irep.iium.edu.my/45991/ http://irep.iium.edu.my/45991/ http://irep.iium.edu.my/45991/1/Endrawan_BBRC_at_PUBMED.pdf
first_indexed	2023-09-18T21:05:27Z
last_indexed	2023-09-18T21:05:27Z
_version_	1777410902344073216

Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis

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